The present invention discloses a laying hen heat stress resistant feed additive. The laying hen heat stress resistant feed additive is made from the following components in parts by weight: 1-3 parts of radix astragali, 0.5-1 part of radix ophiopogonis, 1-3 parts of raw licorices, 0.5-1 part of dandelion, 3-5 parts of fructus forsythiae, 0.1-0.3 part of fructus trichosanthis, 3-5 parts of honeysuckles, 3-5 parts of radix codonopsis, 1-3 parts of rhizoma atractylodis, 3-5 parts of lotus leaves, 0.5-1 part of radix isatidis, 0.3-0.5 part of rosemary, 20-30 parts of potassium chloride, 10-15 parts of sodium hydrogen carbonate, 15-20 parts of bentonite and 5-10 parts of an adaptation promoting agent. The laying hen heat stress resistant feed additive is scientific in ratio, safe and sound, free of drug resistance and residues, and can enhance the adaptability of the laying hens to high temperature, strengthen immune function, coordinate thermal equilibrium and significantly alleviate heat stress reaction. The present invention also discloses a preparation method of the laying hen heat stress resistant feed additive. The preparation method is good in mixing homogeneity, simple in technology and easy to implement, and can applied in a large-scale industrial production.