Mixed feed additive and application thereof
A feed additive and mixed-type technology, which is applied in the field of feed additive preparation, can solve the problems of unreported interaction between γ-aminobutyric acid and Cordyceps strain fermentation products, achieve the best feed additive product and reduce heat stress response. Effect
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Embodiment 1
[0028] 1. Preparation of solid fermented product of Cordyceps guni
[0029] (1) Fermentation strain: Acremonium terricola MKL18 isolated from Cordyceps guni (purchasable from Hefei Maikeluo Biological Engineering Co., Ltd.) was used as the fermentation object.
[0030] (2) Seed cultivation:
[0031] The medium for seed culture is: 600g white granulated sugar, 300g silkworm chrysalis powder, 150g peptone, 200g soybean powder, 100g yeast powder, 10g MgSO 4 , 10g KH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high pressure steam at 121°C for 30min.
[0032] The conditions of seed fermentation are as follows: temperature is 25°C, ventilation rate is 1.00L / (L·min), inoculum size is 5% (mass fraction), liquid volume in 30L airlift fermentation tank is 67%, and fermentation time is 24h.
[0033] (3) Expansion of training:
[0034] The culture medium for expanded cultivation is: 6000g white granulated sugar, 3000g silkworm chrysalis powder, 1500g peptone, 2000g soy...
Embodiment 2
[0050] 1. Pig fattening experiment
[0051] 1.1 Test treatment
[0052] In a pig farm of a large-scale breeding group in Quanjiao, Anhui Province, a 30-day experiment was conducted on fattening pigs in a hot and humid environment (feeding time was 30 days, test time: June 30, 2018-July 30, 2018 ).
[0053] The specific test content is:
[0054] A total of 120 Duroc pigs aged 120 days, with similar weight and quality were selected as the experimental subjects, and were randomly divided into 6 groups, 20 pigs in each group. The feed provided by the following test groups or control groups was used for feeding, and the routine breeding indicators (average daily feed intake, average daily gain, average feed-to-meat ratio) of fattening pigs and the serum stress indicators (cortical Alcohol (COR) and adrenal cortex hormone (ACTH)); Among them, the serum stress index was detected by enzyme-linked immunosorbent assay.
[0055] The specific differences between the test group and the...
Embodiment 3
[0116]This embodiment adopts the same method as in Example 1 to prepare the solid fermented product of Acremonium acremonium; get 40 parts by weight of the solid fermented product of Acremonium acremonium and 50 parts by weight of γ-aminobutyric acid powder and 10 parts by weight of magnesium carbonate powder Mixing is carried out to obtain a feed additive.
[0117] The feed additive was added to the basal ration for feeding mutton sheep, and the addition amount was 0.4‰, and the basal ration containing 0.4‰ of γ-aminobutyric acid powder was used as a control; the results showed that the feed intake, daily The weight gain was higher than that of the control group, and the feed-to-meat ratio was lower than that of the control group.
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