The invention provides a meat substitute stuffing dessert and a preparation method thereof. The meat substitute stuffing dessert comprises main materials and auxiliary materials, wherein the main materials comprise edible mushrooms, textured soybean protein, soybean protein isolate, starch, konjaku flour, edible oil and edible salt. The preparation method comprises the following steps: washing the edible mushrooms, soaking and dicing into small blocks; then stirring and blending the components of the edible mushrooms, the textured soybean protein, the soybean protein isolate, the konjaku flour and the like in a chopping mode, then encrusting, steam cooking, quickly freezing and packaging to obtain the meat substitute stuffing dessert. The preparation method of traditional dessert is changed, and the vegetable diet food materials of the edible mushrooms, the konjaku flour and the like are added to the dessert, so that the nutritional value of the dessert is improved, and the vegetable diet characteristic of the meat substitute stuffing dessert can also be used for preventing and curing a plenty of modern diseases.