The invention provides a method for preparing hulless oat
blood sugar reducing polypeptide with hulless oat albumen
powder. The method comprises the steps that degreased hulless oat albumen
powder is added into pure water at the material to
liquid ratio of 1:(100-500), the pH value is regulated to be 1.0-3.0 with 1M
hydrochloric acid,
pepsin is added according to E / S of 1:(50-100), enzymolysis is carried out for 1-4 h at 37 DEG C, and primary enzymatic
hydrolysate is obtained; the pH value of the primary enzymatic
hydrolysate is regulated with 1M NaoH to be 7.0-8.0, then
trypsin, alpha-
chymotrypsin and
elastase are added according to E / S of 1:(50-100), enzymolysis is carried out for 1-4 h at 37 DEG C, and secondary enzymatic
hydrolysate is obtained; the secondary enzymatic hydrolysate is heated to 95 DEG C for
enzyme denaturalixation for 15 min, spraying
drying is carried out, and polypeptide containing
alpha glucosidase inhibiting activity is obtained. The process is simple, the production cost is low, and the product is safe and free of toxic or side effects. The hulless oat
blood sugar reducing polypeptide prepared through the method can be applied in preparing
medicine or health-care products for preventing and treating diabetes.