30results about How to "Simple extraction and purification process" patented technology

The invention relates to an extraction technology of natural pigment, in particular to a method for extracting purple sweet potato anthocyanin. The method comprises the following steps of: grinding purple sweet potatoes into powder and adding cellulase to carry out enzymolysis on the purple sweet potato powder; adding citric acid to substances subjected to the enzymolysis to carry out extraction;filtering the extract obtained in the extraction step through macroporous absorbing resin, eluting the macroporous absorbing resin which adsorbs purple sweet potato anthocyanin by using eluent and condensing the purple sweet potato anthocyanin subjected to the eluting; and carrying out spray drying on the condensed purple sweet potato anthocyanin pigment obtained in the purification step to obtain final products and detecting the quality of the final products. The purple sweet potato anthocyanin extracted by the method has the advantages of excellent quality, less impurity content, stable property and long shelf life, the number of recycling times of the macroporous absorbing resin is increased, and the production efficiency is improved.

The invention relates to a method for extracting omega-6 polyunsaturated fatty acid from plant seeds, eliminating impurities from the plant seeds, properly preprocessing and then roughly crushing, making microwave vibration to break their walls and rapidly drying and finely crushing to make them into powder of a certain size, making supercritical CO2 fluid extraction on the made powder, selectively extracting, separating and purifying omega-6 polyunsaturated fatty acid in the raw material on proper conditions. The invention fully uses the raw material, and does not produce waste residues and waste steam and pollute the environment. The products have high physiological activity and excellent taste.

The invention relates to purified active polysaccharides extracted from agaricus bisporus can processing waste water and an extracting and purifying technology and application thereof. Monosaccharide components of the purified active polysaccharide Abnp1001 extracted from the agaricus bisporus can processing waste water include arabinose, xylose, mannose, glucose and galactose, and monosaccharide components of the purified active polysaccharide Abnp1002 extracted from the agaricus bisporus can processing waste water include arabinose, xylose, mannose and glucose. The extracting and purifying technology of the active polysaccharides includes the steps of material pretreatment, ion exchange column chromatography and Sephadex G-200 gel column chromatography. The purified and extracted active polysaccharides Abnp1001 and Abnp1002 have good biological activity, and the processing technology is simple.

The invention discloses a process for preparing gardenia iridoid glycosides from the leaves of gardenia plants. The specific process steps are as follows: (1) add beating agent to fresh gardenia leaves, or add extractant to dried gardenia leaf powder to make gardenia leaf slurry; (2) wash gardenia leaf slurry Stir and wash with the agent for more than one time, then filter or centrifuge, combine and retain the supernatant or filtrate to obtain the active ingredient extract of gardenia iridoid glycosides; (3) Concentrate the extract and elute it on a chromatographic column to obtain Gardenia iridoid glycoside compound, off-white or white powder, of which geniposide is 20-99.9%. The method of the invention can prepare a large amount of gardenia iridoid glycoside compounds mainly composed of geniposide at low cost. The present invention utilizes gardenia leaves to produce gardenia iridoid glycoside active ingredients, which can greatly reduce the production cost compared with the traditional production method using gardenia fruit as raw material, and the raw material cost is only 1 / 50 of the traditional cost.

The invention provides a method for extracting ε-polylysine and its salt from fermentation broth. In the acidification tank, the fermentation broth is acidified and then filtered to obtain a clear filtrate, and then the clear filtrate is alkalized to make an alkalized solution Then press it into the resin adsorption column for adsorption, first wash the saturated resin with purified water, and then analyze it with hydrochloric acid to obtain the analysis solution, decolorize the analysis solution with activated carbon, filter to obtain the decolorization solution, and put the decolorization fluid into the membrane filter device for circulation and concentration. The concentrated solution is obtained, and the concentrated solution is sprayed or freeze-dried under reduced pressure to obtain the pure product of ε-polylysine. The raw materials and instruments used in the invention are easy to obtain and low in cost; the extraction and purification process is simple and feasible; and the final product has high yield and high purity. The prepared ε-polylysine can be used as preservative, biopolymer material, microcapsule technology material, drug carrier, food therapy agent, biochip and polymer water-absorbing material, etc.

The invention relates to a preparation method and technology of alliin reference substance, which comprises the following steps of: first extracting alliin from fresh garlic; then preparing the alliinreference substance by using two methods: the first method is to prepare the alliin reference substance by ethanol recrystallization; and the second method is to prepare the alliin reference substance by acetone recrystallization; and finally determining the alliin physical constant, analyzing four spectrum, and determining the purity; if all the indicators thereof are eligible, the product complies with the 'alliin reference substance' required by the 'plant extract reference substance' regulated by the State Food and Drug Administration.

The invention provides malania oleifera lectin and a method for preparing the same. The method comprises the following steps: grinding malania oleifera, stirring and socking the ground malania oleifera into cold water, grading and precipitating by using ammonium sulfate, ultrafiltering to remove salt, concentrating, carrying out ion exchange chromatography, purifying, ultrafiltering to remove salt, concentrating, freezing and drying to obtain the refined malania oleifera lectin. The prepared malania oleifera lectin has the molecular weight of about 60kd, the electrophoresis purity of above 90% and the potency of 1:10<5> or more, namely the HIV infected cell (inhibition concentration 50) IC50 of 1:10<5>, can agglutinate the red blood cells of a rabbit, a pig, a chicken, a duck and a mouse and can be used for preventing HIV infection.