Guanine peptide nucleic acid monomer and synthetic method thereof

Abstract

The invention relates to a guanine peptide nucleic acid monomer. A structural formula of the guanine peptide nucleic acid monomer is as shown in the specification. A preparation method of the guanine peptide nucleic acid monomer comprises the following steps: (1), under a condition that potassium carbonate is taken as alkaline, reacting a compound G0 with benzyl bromoacetate to produce a compound Ga; 2, reacting the compound Ga with triphosgene and reacting the obtained product with diphenyl carbinol to produce Gb; and (3), reacting Gb with cyanosodium ethoxide to produce Gc; and (4), reacting Gc with Fmoc-aminoethyl glycine to produce a target product. According to the synthetic method, the used raw materials are very low in price, and very low in cost, the steps are few, the operation is very simple, the extracting and purifying process is relatively convenient, and the yield and the purity, which respectively reach 98% and over 50%, of the obtained guanine peptide nucleic acid monomer are relatively high. And moreover, the defects in the existing synthetic process are overcome.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and relates to a peptide nucleic acid monomer and a synthesis method thereof, in particular to a guanine peptide nucleic acid monomer and a synthesis method thereof. Background technique [0002] Peptide nucleic acid (PNA) is a chemical substance similar to DNA and RNA, which can be artificially produced for biological research or medical treatment. [0003] The backbone of PNA is composed of repeating N-2-(aminoethyl)-glycine (N-(2-aminoethyl)-glycine) units, which are combined through peptide bonds, and the base and the backbone are separated by subunits. linked by a carbonyl bond. Similar to the polypeptide chain, PNA also has the difference between N-terminal (nitrogen-terminal) and C-terminal (carbon-terminal). [0004] Since PNA does not have a phosphate group like DNA or RNA, there is no electrical repulsion between PNA and DNA, so that the binding strength between the two i...

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Problems solved by technology

[0006] Technical problem to be solved: in the synthetic method of conventional guanine peptide nucleic acid monomer, the process is relatively complicated, the cost of raw materials used in the synthesis is also very high, and the purity and yield of the synthetic guanine peptide nucleic acid monomer are also low , the purity is not more than 95%, and the yield is only 20% to 30%.

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