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A method for extracting ε-polylysine and its salts

A polylysine and extraction method technology, which is applied in the field of extracting ε-polylysine and its salts, can solve the problems of cumbersome extraction process, low content, high cost, etc., and achieve simple extraction and purification process, high purity, and easy instrumentation easy effect

Active Publication Date: 2011-12-14
ZHEJIANG SILVER ELEPHANT BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cumbersome extraction process, low yield, low content, and high cost have always been important factors limiting the industrialization of ε-polylysine. Therefore, the development of a new extraction and purification process has great significance for its industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In the acidification tank, use 30% hydrochloric acid to adjust the pH of the polylysine fermentation broth to 2.0, then heat the acidification solution to 60°C, keep it warm for 10 minutes, cool the acidification solution to 20°C, filter it through a plate frame, and pass through 30% Hydrochloric acid is used to top wash the filter layer and re-filter the plate and frame to obtain a clear filtrate.

[0030] Add 30% sodium hydroxide to the clear filtrate, adjust the pH of the clear filtrate to 9.0 to obtain an alkalized solution. Put the alkalized hydraulic pressure into the 152 resin adsorption column (the ratio of height to diameter is 3:1) for adsorption. During the adsorption process, a certain flow rate should be controlled, and sodium hydroxide should be continuously added to ensure that the pH value of the adsorption process remains at 9 until the resin Adsorption reaches saturation. Wash the saturated resin with purified water until the washing liquid is clear. ...

Embodiment 2

[0033] In the acidification tank, use 30% hydrochloric acid to adjust the pH of the polylysine fermentation broth to 4.0, then heat the acidification solution to 70°C, keep it warm for 20 minutes, cool the acidification solution to 30°C, filter it through a plate frame, and pass through 30% Hydrochloric acid is used to top wash the filter layer and re-filter the plate and frame to obtain a clear filtrate.

[0034] Add 30% sodium hydroxide to the clear filtrate, adjust the pH of the clear filtrate to 10 to obtain an alkalized solution. Put the alkalized hydraulic pressure into the 152 resin adsorption column (the ratio of height to diameter is 3:1) for adsorption. During the adsorption process, a certain flow rate should be controlled, and at the same time, sodium hydroxide should be continuously added to ensure that the pH value of the adsorption process is maintained at 8 until the resin is adsorbed. reached a saturated state. Wash the saturated resin with purified water unt...

Embodiment 3

[0037] In the acidification tank, use 30% hydrochloric acid to adjust the pH of the polylysine fermentation broth to 5.0, then heat the acidification solution to 100°C, keep it warm for 5 minutes, cool the acidification solution to 10°C, filter it through a plate frame, and pass through 30% Hydrochloric acid is used to top wash the filter layer and re-filter the plate and frame to obtain a clear filtrate.

[0038] Add 30% sodium hydroxide to the clear filtrate to adjust the pH of the clear filtrate to 7.2 to obtain an alkalized solution. Put the alkalized hydraulic pressure into the 152 resin adsorption column (the ratio of height to diameter is 2:1) for adsorption. During the adsorption process, a certain flow rate should be controlled, and at the same time, sodium hydroxide should be added continuously to ensure that the pH value of the adsorption process remains at 7.2 until the resin Adsorption reaches saturation. Wash the saturated resin with purified water until the was...

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Abstract

The invention provides a method for extracting ε-polylysine and its salt from fermentation broth. In the acidification tank, the fermentation broth is acidified and then filtered to obtain a clear filtrate, and then the clear filtrate is alkalized to make an alkalized solution Then press it into the resin adsorption column for adsorption, first wash the saturated resin with purified water, and then analyze it with hydrochloric acid to obtain the analysis solution, decolorize the analysis solution with activated carbon, filter to obtain the decolorization solution, and put the decolorization fluid into the membrane filter device for circulation and concentration. The concentrated solution is obtained, and the concentrated solution is sprayed or freeze-dried under reduced pressure to obtain the pure product of ε-polylysine. The raw materials and instruments used in the invention are easy to obtain and low in cost; the extraction and purification process is simple and feasible; and the final product has high yield and high purity. The prepared ε-polylysine can be used as preservative, biopolymer material, microcapsule technology material, drug carrier, food therapy agent, biochip and polymer water-absorbing material, etc.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a method for extracting ε-polylysine (ε-poly-L-lysine, ε-PL) and a salt thereof from a fermentation broth. Background technique [0002] Natural preservatives are new products advocated, developed and sought at home and abroad in recent years. The development of strong antibacterial, safe and non-toxic natural preservatives has become a research hotspot for scientific and technological workers in various countries. Natural preservatives are not only harmless to human health, but also have certain nutritional value, which is the direction of future development. [0003] ε-polylysine is a homopolyamino acid in which L-lysine residues are linked by amide bonds formed by α-carboxyl and ε-amino groups, so it is called ε-polylysine. ε-polylysine has broad-spectrum antibacterial properties, and has good antibacterial effects on Gram-positive bacteria, Gram-negative bac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G69/10
Inventor 周斌陈艺强周桂飞李增亮
Owner ZHEJIANG SILVER ELEPHANT BIO ENG
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