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Active polysaccharide extracted and purified from canned Agaricus bisporus processing wastewater and its extraction and purification process and application

A technology of active polysaccharide and Agaricus bisporus, which is applied to the digestive system, anti-tumor drugs, drug combinations, etc., can solve the problem of wasting beneficial components, and achieve the effects of reducing emissions, simple and easy to implement extraction and purification processes, and reducing pollution levels.

Active Publication Date: 2018-02-23
MINNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Agaricus bisporus is the most widely cultivated edible fungus with the highest output and largest consumption in the world. Its trade is mainly in canned food. During the processing of canned mushrooms, a large amount of them are produced by cleaning, precooking, filling, and liquid injection. Industrial wastewater, which often contains a large amount of soluble substances in the fruiting bodies of Agaricus bisporus, but many canning factories focus on the immediate interests, do not do any treatment on the pre-cooked liquid of Agaricus bisporus, and directly discharge it into the environment. The coordinated development of the environment, ecology, and economy has caused great pressure, and wasted the beneficial ingredients hidden in the wastewater. Therefore, it is necessary to design a method that can extract the beneficial substances from the wastewater produced during the processing of canned Agaricus bisporus. Utilize these beneficial substances to prevent the waste of resources and benefit mankind

Method used

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  • Active polysaccharide extracted and purified from canned Agaricus bisporus processing wastewater and its extraction and purification process and application
  • Active polysaccharide extracted and purified from canned Agaricus bisporus processing wastewater and its extraction and purification process and application
  • Active polysaccharide extracted and purified from canned Agaricus bisporus processing wastewater and its extraction and purification process and application

Examples

Experimental program
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Embodiment 1

[0052] An extraction and purification process for active polysaccharide Abnp1001, active polysaccharide Abnp1002, Abap1001, Abap1002, which comprises the following process steps:

[0053] (1) Material pretreatment: collect the industrial wastewater generated during the processing of canned mushrooms, and then concentrate the industrial wastewater by rotary evaporation until the refractive index of the solution reaches 35% to obtain the mushroom pre-boiled concentrate; measure the mushroom per unit volume Pre-boil the concentrated liquid, add an equal volume of deionized water to the mushroom pre-boiled concentrated liquid for mixing, and then completely dissolve the crystals precipitated in the mushroom pre-boiled concentrated liquid in a water bath at 75°C. After dissolving, centrifuge and centrifuge The speed of the machine is controlled between 11500rpm, the centrifugation temperature is 25°C, and the centrifugation time is 10min. After centrifugation, the supernatant is tak...

Embodiment 2

[0069] An extraction and purification process for active polysaccharide Abnp1001, active polysaccharide Abnp1002, Abap1001, Abap1002, which comprises the following process steps:

[0070] (1) Material pretreatment: collect the industrial wastewater generated during the processing of canned mushrooms, and then concentrate the industrial wastewater by rotary evaporation until the refractive index of the solution reaches 40% to obtain the mushroom pre-boiled concentrate; measure the mushroom per unit volume Pre-boil the concentrated liquid, add an equal volume of deionized water to the mushroom pre-boiled concentrated liquid for mixing, and then completely dissolve the crystals precipitated in the mushroom pre-boiled concentrated liquid in a water bath at 70°C. After dissolving, centrifuge and centrifuge The speed of the machine is controlled at 12000rpm, the centrifugation temperature is 20°C, and the centrifugation time is 15min. After centrifugation, the supernatant is taken, a...

Embodiment 3

[0086] An extraction and purification process for active polysaccharide Abnp1001, active polysaccharide Abnp1002, Abap1001, Abap1002, which comprises the following process steps:

[0087] (1) Material pretreatment: collect the industrial wastewater generated during the processing of canned mushrooms, and then concentrate the industrial wastewater by rotary evaporation until the refractive index of the solution reaches 55% to obtain the mushroom pre-boiled concentrate; measure the mushroom per unit volume Pre-boil the concentrated liquid, add an equal volume of deionized water to the mushroom pre-boiled concentrated liquid for mixing, and then completely dissolve the crystals precipitated in the mushroom pre-boiled concentrated liquid in a 65°C water bath, and then centrifuge in a centrifuge after dissolving. The speed of the machine is controlled at 12500rpm, the centrifugation temperature is 15°C, and the centrifugation time is 20min. After centrifugation, the supernatant is t...

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Abstract

The present invention relates to the active polysaccharide extracted and purified from Agaricus bisporus canned wastewater and its extraction and purification process and application. The active polysaccharide Abap1001 extracted and purified from Agaricus bisporus processed wastewater is composed of arabinose and xylose as monosaccharides , mannose and glucose, the active polysaccharide Abap1002 extracted and purified from the canned processing wastewater of Agaricus bisporus, its monosaccharide composition is arabinose, xylose, mannose and glucose, an extraction and purification process of active polysaccharide, which includes the following Process steps: material pretreatment, ion exchange column chromatography, Sephadex G-200 gel column chromatography, the active polysaccharide Abap1001 and the active polysaccharide Abap1002 of the present invention have good biological activity, and the purification process of the present invention is simple and easy.

Description

technical field [0001] The invention relates to an active polysaccharide extracted and purified from canned processing wastewater of Agaricus bisporus, an extraction and purification process and application thereof. Background technique [0002] Agaricus bisporus is flat in nature and sweet in taste, can nourish the spleen and nourish qi, moisten dryness and resolve phlegm, has nourishing effect, digests food, clears the mind, calms liver yang, and is mainly used for indigestion and high blood pressure. Agaricus bisporus is thick in meat and delicious in taste. It is low in heat energy and has a high health care effect. It is praised as the health food of the 21st century by the Food and Agriculture Organization of the United Nations. The fruiting bodies of Agaricus bisporus are rich in amino acids, mineral elements, polysaccharides, nucleotides, crude protein, crude fat, and crude fiber. , ash and its superoxide dismutase (SOD). [0003] Agaricus bisporus can reduce total ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P1/16A61P35/00
Inventor 潘裕添黄家福
Owner MINNAN NORMAL UNIV
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