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Method for extracting purple sweet potato anthocyanin

A purple sweet potato anthocyanin and extraction method technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problem of low quality of purple sweet potato anthocyanin pigment and low extraction rate of purple sweet potato anthocyanin pigment , easy to cause pollution and purification equipment and other problems, to achieve the effect of optimizing the extraction and purification process, high quality, and reduced impurity content

Active Publication Date: 2013-09-25
湖北紫鑫生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the direct extraction of fresh potatoes, this method has relatively many impurities, which is easy to cause pollution and damage to the purification equipment, which will have a negative impact on subsequent purification, and the extraction rate of purple sweet potato anthocyanin pigments in the prior art is relatively low. , resulting in a large waste; the quality of the purple sweet potato anthocyanin pigment extracted in the prior art is not high, so the purple sweet potato anthocyanin pigment market has been in a state of high price and low quality

Method used

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  • Method for extracting purple sweet potato anthocyanin
  • Method for extracting purple sweet potato anthocyanin
  • Method for extracting purple sweet potato anthocyanin

Examples

Experimental program
Comparison scheme
Effect test

experiment Embodiment 1

[0029] Experimental Example 1: Set the pH values ​​of the anthocyanin pigment solutions to 4.0, 4.5, 5.0, 5.5, and 6.0 respectively, and other conditions are temperature 25°C, enzyme addition 1.5%, substrate concentration 1:5, and enzymatic hydrolysis time 30min, detect the absorbance of anthocyanin pigment solution after enzymatic hydrolysis, and calculate the color value of anthocyanin pigment solution, draw figure 2 The curve shown. Depend on figure 2 It can be seen that acidic conditions are conducive to the enzymatic hydrolysis of purple sweet potato cell membrane by cellulase, thereby releasing more anthocyanin pigments.

experiment Embodiment 2

[0030] Experimental Example 2: Set the enzymolysis extraction reaction time to 15min, 30min, 45min, 60min, 75min respectively, other conditions are temperature 25°C, enzyme addition 1.5%, substrate concentration 1:5, pH value 4.0, enzymolysis Detect the absorbance of anthocyanin pigment solution after finishing, and calculate the color value of anthocyanin pigment solution, draw image 3 The curve shown. Depend on image 3 It can be seen that the color value decreases after 30 minutes of reaction. This is because the cellulase acts for a long time and destroys the anthocyanin pigment, so the most suitable reaction time for enzymatic hydrolysis is determined to be 30 minutes.

experiment Embodiment 3

[0031] Experimental Example 3: Set the enzymolysis extraction reaction temperature to 25°C, 30°C, 35°C, 40°C, 45°C respectively, other conditions are reaction time 30min, enzyme amount 1.5%, substrate concentration 1:5, pH Value 4.0, detect the absorbance of anthocyanin pigment solution after enzymolysis, and calculate the color value of anthocyanin pigment solution, draw Figure 4 The curve shown. Depend on Figure 4 It can be seen that the color value is the lowest when the temperature is 30°C, indicating that the cellulose destroys anthocyanin pigments at this temperature. After that, the extraction rate increases with the increase of temperature, but the increase is slow after 40°C. In order to ensure a high extraction rate and make the present invention more energy-saving, 40° C. is the optimum temperature.

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Abstract

The invention relates to an extraction technology of natural pigment, in particular to a method for extracting purple sweet potato anthocyanin. The method comprises the following steps of: grinding purple sweet potatoes into powder and adding cellulase to carry out enzymolysis on the purple sweet potato powder; adding citric acid to substances subjected to the enzymolysis to carry out extraction;filtering the extract obtained in the extraction step through macroporous absorbing resin, eluting the macroporous absorbing resin which adsorbs purple sweet potato anthocyanin by using eluent and condensing the purple sweet potato anthocyanin subjected to the eluting; and carrying out spray drying on the condensed purple sweet potato anthocyanin pigment obtained in the purification step to obtain final products and detecting the quality of the final products. The purple sweet potato anthocyanin extracted by the method has the advantages of excellent quality, less impurity content, stable property and long shelf life, the number of recycling times of the macroporous absorbing resin is increased, and the production efficiency is improved.

Description

technical field [0001] The invention relates to a technology for extracting natural pigments, in particular to a method for extracting purple sweet potato anthocyanin pigments. Background technique [0002] As people pay more attention to food safety, the state has stepped up efforts to crack down on the illegal use of synthetic pigments in food and cosmetics, which has given people a new understanding of the irreplaceable superiority of natural pigments. Purple sweet potato anthocyanin is an important natural pigment discovered at the end of the last century and widely used in food, cosmetics and health products in developed countries. Purple sweet potato anthocyanin pigment is a mixed pigment of various anthocyanins, which not only has better light and heat stability than other natural pigments, but also has a strong ability to resist liposome peroxidation and scavenge hydroxyl free radicals in the human body. ability. Natural purple sweet potato anthocyanin has many adv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00
CPCC09B61/00A23L1/2751A23L5/43
Inventor 周树勇
Owner 湖北紫鑫生物科技有限公司
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