Method for preparing gamma-aminobutyric acid by using continuous circulation of 732 resin to assist immobilized cells

A technology of immobilizing cells and aminobutyric acid, applied in the field of green biomanufacturing, can solve the problem of high cost of biosynthesis of GABA

Active Publication Date: 2017-11-14
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

[0009] The object of the present invention is to provide a kind of 732 cationic exchange resin columns and immobilized Enterococcus faecium cell column coupling to form the cycle compound transformation reaction system, through the promoting action of 732 cationic exchange resins to the activity of Enterococcus faecium GAD, finally realize the improvement The purpose of GABA production is to solve the problem of high cost of biosynthesis of GABA, and to solve the problem of continuous production of immobilized Enterococcus faecium cell columns by using two 732 cation exchange resin columns alternately

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  • Method for preparing gamma-aminobutyric acid by using continuous circulation of 732 resin to assist immobilized cells

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Embodiment 1

[0052] A method for preparing gamma-aminobutyric acid by using 732 resin continuous circulation to assist immobilized cells according to the present invention mainly includes the following steps:

[0053] ①Refer to the cell immobilization method of Jiao Yang, Wang Jianmin and Yang Shengyuan in the abstract (Journal of Nuclear Agriculture, 2009, 23(6):1026-1031), and prepare immobilized Enterococcus faecium GDMCC 60203 by calcium alginate cell embedding method cells, and then the immobilized Enterococcus faecium GDMCC 60203 cells were packed into a column with 0.2mol / L acetic acid-sodium acetate buffer solution at pH 4.2 to prepare an immobilized Enterococcus faecium GDMCC 60203 cell reaction column;

[0054] ②The 732 cation exchange resin was regenerated or pretreated according to the instructions of the resin, and then packed with 0.2mol / L acetic acid-sodium acetate buffer solution with pH 4.2 to prepare the 732 cation exchange resin assisted reaction column, 732 cation exchan...

Embodiment 2

[0066]A method for preparing gamma-aminobutyric acid by using 732 resin continuous circulation to assist immobilized cells according to the present invention mainly includes the following steps:

[0067] ① Referring to the cell immobilization method of Shen Yu et al. in the abstract (Fine Chemical Industry, 2008, 25(5):459-462), the immobilized Enterococcus faecium GDMCC 60203 cells were prepared by embedding with carrageenan and gelatin, and then the fixed Enterococcus faecium GDMCC60203 cells were packed with 0.2mol / L acetic acid-sodium acetate buffer solution with pH 4.2 to prepare the immobilized Enterococcus faecium GDMCC 60203 cell reaction column;

[0068] ②The 732 cation exchange resin was regenerated or pretreated according to the instructions of the resin, and then packed with 0.2mol / L acetic acid-sodium acetate buffer solution with pH 4.2 to prepare the 732 cation exchange resin assisted reaction column, 732 cation exchange resin assisted reaction column Set up two,...

Embodiment 3

[0080] A method for preparing gamma-aminobutyric acid by using 732 resin continuous circulation to assist immobilized cells according to the present invention mainly includes the following steps:

[0081] ① Referring to Zhao Weirui et al.’s abstract (Journal of Chemical Engineering of Universities, 2015, 29(1):138-144), firstly, an organic solvent with low dielectric constant (0.68) was used to treat Enterococcus faecium GDMCC 60203 cells were permeabilized, and then referring to the cell immobilization method of Jiao Yang et al. Enterococcus GDMCC 60203 cells, and then the immobilized Enterococcus faecium GDMCC 60203 cells were packed into a column with a 0.2mol / L acetic acid-sodium acetate buffer solution at pH 4.2 to prepare an immobilized Enterococcus faecium GDMCC60203 cell reaction column;

[0082] ②The 732 cation exchange resin was regenerated or pretreated according to the instructions of the resin, and then packed with 0.2mol / L acetic acid-sodium acetate buffer soluti...

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Abstract

The invention relates to a method for preparing gamma-aminobutyric acid by using continuous circulation of 732 resin to assist the immobilized cells, and belongs to the technical field of green bio-manufacturing. The method is characterized in that 732 cation exchange resin columns I and II arranged in parallel are respectively and alternatively used as auxiliary reaction columns by valve control to be coupled with immobilized enterococcus faecium cell columns, and are connected with other auxiliary facilities or equipment to form a circular reaction system; high-efficiency catalytic reaction is carried out by circulation of primers between the 732 resin auxiliary reaction columns and the immobilized enterococcus faecium cell columns, so that the yield of gamma-aminobutyric acid is increased; and while in conversion reaction assisted by one 732 resin column, the other 732 resin column is diluted, regenerated and balanced, so that continuous production of the immobilized enterococcus faecium cell columns under assistance of the 732 resin columns is realized. Compared with a single-column reaction method only adopting the immobilized cells, the method provided by the invention has the advantage that under the conditions of same reaction time and equivalent immobilized enterococcus faecium, the GABA yield in single-batch circulation is increased by 178.83%.

Description

technical field [0001] The invention relates to a method for preparing gamma-aminobutyric acid by assisting immobilized cells in continuous circulation with 732 resin, which belongs to the field of green biomanufacturing. Background technique [0002] γ-Aminobutyric acid (γ-Aminobutyric acid, GABA) is a non-protein amino acid with 4 carbon atoms. It is the main inhibitory neurotransmitter in the mammalian central nervous system. Antioxidant, anti-inflammatory, anti-cancer, blood pressure lowering, sedative and sleep improving effects have become the ingredients of pharmaceuticals and health products that have attracted much attention. GABA is also an important raw material for the production of bioplastic polyamide 4 due to the formation of 2-pyrrolidone by lactamation. [0003] Microbes grow and multiply quickly, and their production of GABA is not limited by time and space. Therefore, the production of GABA by microorganisms GAD (Glutamate decarboxylase, GAD, EC4.1.1.15) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12N11/00C12R1/46
CPCC12N11/00C12P13/005
Inventor 杨胜远韦锦
Owner LINGNAN NORMAL UNIV
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