38results about How to "Save experimental materials" patented technology

The invention discloses an oxygen index tester which comprises an oxygen channel, a nitrogen channel, a controller module and a human-machine interface module, wherein the oxygen channel and the nitrogen channel are both connected with a gas mixing cylinder, and then connected with a combustion cylinder through a temperature controller and an oxygen sensor; the oxygen channel and the nitrogen channel are both provided with a flow switch valve and a quality flow controller; a temperature sensor is arranged in the temperature controller; the controller module is connected with a control input end of the temperature controller; the detection values of the temperature sensor in the temperature controller and the oxygen sensor are collected at the same time, and the detection values are output to the human-machine interface module; the human-machine interface module comprises a display part, an input part and a control part, wherein the input part inputs a control quantity related parameter value to the control part, and the control part respectively calculates the control quantity of oxygen and nitrogen according to the related parameter value and then outputs the control quantity to the controller module; and the controller module controls the quality flow controller according to the received control quantity so as to achieve the aim of automatically adjusting and controlling as well as improving the test precision.

The invention relates to a simulation device used for simulating migration of horizontal well annulus rock cuttings in a petroleum pneumatic drill. The simulation device comprises an air feed part, a rock cuttings supplying part, a downhole annulus simulation part, a water supplying part, a separation and recycle part and a data acquisition part. According to the simulation device used for simulating migration of the horizontal well annulus rock cuttings in the petroleum pneumatic drill, air rock cuttings migration experiments can be conducted under a low pressure circumstance, visualized observation of the migration of the air rock cuttings can be conducted, and the rock cuttings migration situations under various factors of working conditions including effluent of water in a stratum can be simulated; meanwhile, through the separation and recycle part of the simulation device, the rock cuttings in the simulation experiments can be repeatedly utilized.

The invention relates to a rapid evaluation method for a low-cycle fatigue performance of ultrathin automobile steel plate. By the method, the low-cycle fatigue lifetime of the material is evaluated according to a fatigue lifetime test result of a thin foil sample of the plate under a symmetric flexural fatigue loading method of cantilever, and the evaluation of strain control fatigue loading andfatigue lifetime of the ultrathin automobile steel plate is achieved by building a relation between the flexural deflection of the thin foil sample and maximum stress and selecting a thickness of thethin foil sample. By the method, the fatigue cycle lifetime of the ultrathin automobile steel plate under a large strain condition can be tested, and the rapid evaluation method is provided for the low-cycle fatigue lifetime test which is difficult to acquire under an axial pull-press fatigue loading condition of the ultrathin automobile steel plate. The method can be further expanded and appliedto low-cycle fatigue performance evaluation of the other ultrathin metal plates of which axial pull-press fatigue loading experiments are difficult to perform.

The invention discloses a method for high-flux screening of host genes essential for plant virus copying, belongs to the technical field of gene engineering and aims to solve the problem that at present, an infective cloning construction method is low in virus infection efficiency and make gene expression difficult to control. The method for high-flux screening of host genes essential for plant virus copying specifically comprises the steps that an inducible promoter is utilized to establish inducible virus infection cloning, transgenic plants are used for constructing a mutant library throughagrobacterium tumefaciens-mediated transformation plants, an inducer is utilized to induce virus infection after mutants germinate, the mutants are screened to obtain mutant plants which cannot be induced by viruses, and the host genes essential for plant virus copying are positioned and cloned by cooperating with a map-based cloning and whole genome sequencing method.

The invention discloses a biaxial drawing-pressing test part with a matrix fiber bundle. The biaxial drawing-pressing test part comprises a long strip-shaped sample and is characterized in that the long strip-shaped sample comprises a fiber cloth deposited matrix, the middle of the long strip-shaped sample is a test part, two ends of the long strip-shaped sample are clamping parts, the test part only contains warp-wise fibers circularly distributed along a long strip-shaped sample width direction, the clamping parts are braided structures, anti-drag rubber parts are fixed to two sides of the test part under compressive load, the length of the anti-drag rubber parts is the same to the length of the test part, and a compressive loading head is fixed to the outer side of each one of the anti-drag rubber parts. The biaxial drawing-pressing test part has the advantages of simple preparation processes, low experiment cost, good test part quality and low compressive instability.

The invention provides a preparation method of a Saccharum arundinaceum root-tip meristematic zone chromosome specimen, comprising: root-tip culture, root-tip sampling and pretreatment, root-tip enzymolysis, chromosome preparation, microscopic examination and other steps; the preparation method is characterized in that a whole-plant culture root system is planted in a bucket, the root tip is treated with 0.002 M of 8-hydroxyquinoline solution at 25-28 DEG C for 3-4 h, and the specimen is prepared by an enzymolysis wall degradation hypotonic method. The preparation method is highly operable, allows high-active meristematic zone root tip to be easily obtained, takes shorter time for root-tip culture, is low in manpower and material resource consumption and high in preparation efficiency; many interphases of meristematic zone cells are obtained; the obtained chromosome has vivid form and good dispersion uniformity, and is suitable particularly for the preparation of Saccharum arundinaceum chromosome specimens; the preparation method is also applicable to the preparation of chromosome specimens of sugarcane and plants in its related genera.

The invention discloses a hepatoma cell line for conditionally-induced knockout overexpressed Chd1l genes and a construction method thereof. The method comprises the steps that firstly, a hepatoma cell line for overexpressed Chd1l and green fluorescent proteins is constructed; secondly, a carrier for conditionally-induced expressed Cas9 is transferred into the hepatoma cell line; finally, a pLVX-mCherry-hU6-Chd1l-SgRNA carrier with red fluorescence and targeted Chd1l cutting activity is constructed and transferred to the cell line, and the hepatoma cell line for conditionally-induced knockoutoverexpressed Chd1l is obtained at last. The cell line has the advantages of being the hepatoma cell line for overexpressed Chd1l before the induction of doxycycline (Dox) and being the hepatoma cellline for expressed knockout Chd1l after induction. The cell line has good contrast, the simultaneous culture of multiple cell lines can be reduced, the scientific research work is simplified, and thecell line is an ideal tool for gene function research.

The invention discloses a pipetting device suitable for continuous sample adding. The pipetting device comprises a shell, a piston assembly, a pushing device, a sample feeding assembly and a countingassembly, wherein the piston assembly, the pushing device and the sample feeding assembly are arranged in the shell; the sample injection assembly comprises a sample tube, a liquid outlet pump and a liquid eduction tube, the liquid eduction tube is in through connection with a pump opening of the liquid outlet pump, the liquid eduction tube vertically extends out of the shell, the liquid outlet pump is connected to a cover body of the sample tube, and the top of the liquid outlet pump is connected with the push rod; the counting assembly comprises a counter and a touch pressing assembly, the counter is fixed to the shell, the touch pressing assembly is connected to the shell, the counter comprises an outer shell and a counting disc sequence composed of two counting discs, a display windowis formed in the outer shell, a counting button is arranged at the top of the outer shell, and a reset knob is arranged on the right side of the outer shell. The pipetting device is mainly designed for the 96-hole trough plate sample adding process, the error rate can be remarkably reduced, and the accuracy of an operation result is guaranteed.

The invention relates to application of bacteriophage in verification of a biological product virus removal process. According to the invention, bacteriophage is used as an indicator virus in verification of a virus removal process, and the indicator virus has the advantages of small size, high titer and simple purification; the detection requirements of biological product verification laws and regulations are met by adding a small amount of bacteriophage; experimental materials are saved; the detection time is short; the process verification speed is accelerated; and the economic cost and thelabor cost are saved.

The invention discloses an experimental method for preparing organic matters from green leaves under light, wherein the experimental method comprises the steps: selecting two pots of Chinese cabbage buds, respectively numbering the two pots of Chinese cabbage buds as a pot A and a pot B, covering the two pots of Chinese cabbage buds with lightproof boxes for dark treatment, taking off the box fromthe pot A, culturing in normal sunlight, and continuously covering the pot B with the box for shading culture; putting the leaves of the two pots of Chinese cabbage buds into two beakers respectively, adding alcohol with the concentration of 95% into each beaker, and then heating in a water bath until the leaves turn into transparent color from green; taking out the transparent Chinese cabbage bud leaves, putting the leaves into a beaker containing clear water, stirring the leaves with a glass rod attached to the leaves for 2-3 times, taking out the leaves, putting the leaves into a clean culture dish for dyeing, and dropwise adding an iodine solution into the leaves for dyeing; observing the change of the leaves of the Chinese cabbage buds after the iodine solution is dropwise added, andrecording experimental results. The method has the characteristics that experimental materials are easy to obtain and culture, the experimental cost can be saved, the experimental process is simple to operate, and the experimental effect is obvious.

The invention discloses a method for detecting an immune regulation function of neural stem cells. The method comprises the following steps of (1) conducting adherent culture of suspended neural spheres; (2) inactivating the neural stem cells; (3) marking hPBMC by CFSE; (4) conducting co-culture; (5) conducting lymphocyte subpopulation and proliferation detection; (6) conducting cytokine detection; (7) conducting data processing; and (8) conducting result analysis. According to the method, the hPBMC is utilized to simulate human immune response in vitro, and immunomodulatory effects of the neural stem cells with different sources, different generations, different culture modes and the like are studied, so that a basis is provided for process development of the neural stem cells. Accordingto the method, the nerve spheres subjected to suspension culture are digested into single cells, and then wall attachment is carried out, so that quantity control and inactivation of the single cellsare facilitated, and the proliferation condition of specific cell subsets can be visually detected by using a flow cytometry in combination with different lymphocyte subset antibodies by utilizing thecharacteristic that the CFSE is averagely distributed in a filial generation along with cell division. Various detection indexes are combined, so that operation steps are reduced, experimental materials are saved, and the cost is reduced.

The invention discloses an anti-influenza virus or anti-inflammatory drug screening model based on living lung slices, which is characterized in that it comprises the following steps: first infecting the living lung slices with the virus, and then applying the drug to be tested to the virus-infected Live lung slices, and finally verify the effect of the drug to be tested by detecting changes in virus titers and related cytokines. The invention also discloses a method for constructing the screening model, which includes: preparation of lung slices, optimization of thickness of lung slices, evaluation of the correlation between neuraminidase activity and virus titer, and selection of inflammatory factors. Through this invention, a drug screening method based on living lung slices is constructed, which is more accurate and effective than traditional cell models in line with real body conditions.

The invention discloses an in-vitro leaf section identification method for resistance of wheat to stripe rust. The method is as below: 8-12 days after wheat seeds to be detected are sown, when first leaves of wheat seedlings are completely unfolded and core leaves are just exposed, the first leaves of the wheat seedlings are cut into leaf sections with the length of 2-3 cm on an ultra-clean workbench, and the front faces of the leaves are flatly laid on the surface of a 0.5% water agar culture medium containing 60-80 mg / L benzimidazole upwards; the fresh summer spores of the puccinia striiformis and 0.05% Tween 20 aqueous solution are uniformly mixed, the cut leaf sections are sprayed and inoculated with the mixture; the inoculated wheat leaf segments are transferred into an illumination incubator, moisture is preserved for 24 hours under the conditions that the temperature is dark and the humidity is 100%, and then culturing is conducted for 14-16 days under the conditions that the illumination intensity is 6000 Lux-8000 Lux and the relative humidity is 60%-80%; and the reaction types of the wheat stripe rust are distinguished according to 0-4 levels of standards. Compared with the prior art, the wheat stripe rust resistance condition can be quickly identified according to the wheat stripe rust reaction types by inoculating and culturing the wheat in-vitro leaves with the wheat stripe rust, time and labor are saved, the influence of environmental conditions is avoided, experimental materials are saved, the space is saved, and the method is simple, convenient and quick.

The invention relates to an electromechanical test device, in particular to a device capable of conveniently testing part of performance of other devices or installed in a complete set, and belongs tothe field of electromechanical manufacturing, wherein a rotatable connecting shaft is arranged on the housing; the connecting shaft is provided with an electromechanical device and a pinion device; the electromechanical device comprises a centrifuge device, a rotary filter device, and a device with a stator and a rotor; the pinion device is selected from any one of a transmission gear and a ratchet wheel, and is mashed with a big gear wheel and the like.

The invention discloses a fatigue wear testing clamp capable of accurately regulating and controlling a radial deformation amount of a flexible wheel and a testing method, aiming at solving the problem that the radial deformation amount of the flexible wheel in a whole machine test cannot be controlled and measured, and realizing accurate regulation and control of the radial deformation amount ofthe flexible wheel. The supporting of a vernier caliper on a short-cylinder round ring can be used for accurately simulating the supporting of a long-shaft outer ring of a wave generator on an inner wall of the flexible wall; the radial elongation of the vernier caliper is adjusted in a variable gradient manner or the wall thickness of the short-cylinder round ring is adjusted in the variable gradient manner; an influence rule of the size of a long shaft of the wave generator and the wall thickness of the flexible wheel on the fatigue wear of the flexible wheel can be researched, and an actingmechanism of influencing the fatigue wear of the flexible wheel is deeply explored. The clamp disclosed by the invention has the advantages that the material of the flexible wheel is saved, the testing efficiency is improved and a more direct experiment result also can be obtained.

The invention relates to a sand box physical simulation experiment device for simulating pre-existing fracture superimposed oblique-slip deformation, which includes a support mechanism, a model machine and a computer control system, the support mechanism includes a base, a base slide rail, a fixed platform base, an adjustment Hydraulic cylinders, cross-type lifting brackets, variable knuckles, and pneumatic support rods; model mechanisms include inclined slide plates, retractable angle scales, adjustment plates, fixed platens, fixed plate bases, adjustment slide rails, and drive hydraulic cylinders; drive hydraulic pressure The cylinder and the adjusting hydraulic cylinder are connected with the computer control system; the hydraulic cylinder is driven by computer control, and then the movement speed and movement time of the inclined sliding table are controlled to realize two-dimensional or three-dimensional, multi-phase superimposed deformation simulation of different inclined sliding directions and fault dip angles experiment. The invention can simulate deformation processes of different properties through a single experiment; it can also continuously simulate the superimposed evolution process of multi-stage deformations in one experiment without repeatedly disassembling models and debugging equipment.

The invention discloses an adjustable rotatable trapezoid similar model test apparatus, which includes a model support comprising a front baffle plate, a back baffle plate, a left side column , a right side column, a top plate and a base plate. The lower ends of the left side column and the right side column are equipped with a rotating shaft, the angle between the left side column and the base plate and the angle between the right side column and the base plate are both 90 to 120 degrees. Both the left side column and the right side column are hinged to the base plate through the rotating shaft. A simulation zone is between the front baffle plate and the back baffle plate. Both the front baffle plate and the back baffle plate are perpendicular to the base plate. A rotation bearer is arranged on the model support bottom near the left side column. A coal seam is simulated by a crawler belt which is detachable and is supported by pillars. Exploitation process is simulated by lowering small pillars hop-by-hop. Supplementary parts used in model support fixing, rotating and migrating are also included. The apparatus is suitable for exploitation simulation research on horizontal, inclined and steeply inclined coal seam in a lab, without need to arrange a pit cooperating with the rotation of the model on a lab ground or enlarging the space above the lab.

The invention discloses a high-efficiency rapid propagation method of good Rhus chinensis strains. The high-efficiency rapid propagation method comprises the following steps: performing root-pressing propagation on the good field perennial Rhus chinensis earth surface root and culturing for 25-40 days to grow a complete plant; carrying out surface sterilization on leaves of the seedling of the complete plant, taking leaf vein and inoculating on an induction medium, performing illumination culture for 28-35 days to enable the two ends of the leaf vein to grow adventitious buds; inoculating the adventitious buds on a multiple-shoot proliferation and extension medium for bud proliferation and extension, transferring to a rooting medium for rooting after buds grow for 2-3cm, thereby obtaining the strong Rhus chinensis test-tube plantlet. The high-efficiency rapid propagation method has the advantages that root-pressing propagation is adopted to strength the seedling, the problem of pollution and browning severity caused by a situation that field materials are directly drawn as the explants is solved, and the method is convenient for realizing regeneration and obtaining tissue culture seedlings.

The invention discloses a reliability experiment device suitable for a multi-material multi-sample honing machine, and aims to solve the problem that a honing machine is difficult to simulate and loadat present. The experiment device comprises a cutting system, a deceleration system, a cooling system, a support, a moving platform, a clamping mechanism, a measurement processing system and a transmission mechanism; the transmission mechanism is installed on the ground through an experiment platform bed body; the moving platform is installed on the experiment platform bed body through a movableplatform therein; the movable platform and a movable platform guide rail are assembled and are in sliding connection; the clamping mechanism is installed on the experiment platform bed body through afirst rotating pressing piece supporting frame and a second rotating pressing piece supporting frame; the measuring system is arranged on the experiment platform bed body and the 15 positioning pins;and the cutting system and the deceleration system are installed on the experiment platform bed body through a support, the cooling system is installed on the ground through a sealed water tank in thecooling system, and the processing system is installed on the ground on left of the experiment platform bed body through a computer desk.

The invention discloses a connection method for silicon nitride ceramic / nickel-based superalloy, and relates to a connection method for ceramic / superalloy. The invention aims to solve the technical problem of thermal mismatch of connection of ceramic and high-temperature alloy at present. The method is easy to operate, direct brazing of the ceramic and the metal is achieved without any modification treatment on the surface of the sample to be welded before welding, and effective connection of the ceramic and the metal can be achieved by adding the middle layer; the Ni layer in the middle layer can be connected with the nickel-based high-temperature alloy without adding an auxiliary layer under the condition that the requirement for isothermal solidification of the ceramic side is met, the complexity of sample assembly is simplified, and experimental materials are saved.

The invention provides a dyeing method for observing the internal microstructure of plants, comprising: (1) slicing by hand; (2) putting the slicing by hand into glycerin and storing it for later use; (3) placing the slicing by hand on a glass slide, and adding After staining the freehand sections with toluidine blue, add water dropwise and observe the aerenchyma under a microscope; (4) add phloroglucinol for staining, then add concentrated hydrochloric acid for staining, and observe lignification under a microscope; After washing with 75% ethanol, add Sudan 7B dropwise for staining, then add water dropwise, and observe suberization under a microscope; Water was added dropwise, and the Caspian bands were observed under a fluorescence microscope. This method can store freehand slices in glycerin and use them anytime, which makes the experiment time more flexible, and only one intact slice can be stained and observed four times successively, which reduces the workload of freehand slices, saves experimental materials, and improves the experimental efficiency.

The invention discloses a biaxial drawing-pressing test part with a matrix fiber bundle. The biaxial drawing-pressing test part comprises a long strip-shaped sample and is characterized in that the long strip-shaped sample comprises a fiber cloth deposited matrix, the middle of the long strip-shaped sample is a test part, two ends of the long strip-shaped sample are clamping parts, the test part only contains warp-wise fibers circularly distributed along a long strip-shaped sample width direction, the clamping parts are braided structures, anti-drag rubber parts are fixed to two sides of the test part under compressive load, the length of the anti-drag rubber parts is the same to the length of the test part, and a compressive loading head is fixed to the outer side of each one of the anti-drag rubber parts. The biaxial drawing-pressing test part has the advantages of simple preparation processes, low experiment cost, good test part quality and low compressive instability.

The invention provides a method for predicting a crack initiation stress by combining a small taper experiment with simulation. The method comprises the following steps: preparing a plurality of groups of small-taper samples and traditional parallel samples; carrying out a tensile experiment on the traditional parallel samples to obtain an engineering stress-strain curve of the samples; setting a required stress under a dead load condition according to the measured yield strength of the material; setting a deadline in a dead load state, then taking out the samples, cutting the samples along a central axis, and counting the depth of the crack and the relative position of the crack to the centers of the samples by using a scanning electron microscope; and deducing the distance from the crack initiation part to the centers of the samples according to the crack depth and the distance to the centers. A stress distribution cloud chart of the samples in a stressed state is simulated by using a finite element to obtain the stress at the crack initiation part so that the stress value of crack initiation can be predicted by using the small-taper samples. According to the invention, an experimental period is greatly shortened, and experimental materials are saved.

The invention provides a tissue-culture rapid propagation method for moringa. The method comprises the steps of disinfection and germination of moringa seeds, adventitious bud induction, subculture propagation and rooting culture, thereby completing the tissue-culture rapid propagation of the moringa. According to the method, the rapid propagation of the moringa is carried out in an organ propagation mode without a dedifferentiation process, so that chromosomal variation caused in the dedifferentiation process is avoided, and the stability of heredity is high; by a bud propagation mode, the propagation coefficient is large, the propagation speed is high, and thus, relatively high requirements of the market on moringa seedlings can be met. According to the method, development and innovation are carried out on the basis of tests of predecessors, and adopted culture medium types and culture conditions are more beneficial to the induction and propagation of adventitious buds of the moringa and also are more beneficial to factory operation.

The invention discloses an early evaluation method for the salt tolerance of the willow coastal mud flat. The method comprises the following steps of: taking a one-year old twig from the limb; diluting sea water into solutions with different mass concentrations of salt content; pouring the solutions with different concentrations into a black plastic water tank respectively; making a cottage plate; inserting the twig into the cottage plate; and culturing the water tank in an illumination culture box. The method disclosed by the invention is easy to master and convenient and quick and basicallynot limited by external environment, and can be implemented all year round; the evaluation speed is high, and generally the evaluation result can be obtained in one week; and moreover, the method requires few experimental materials, has small error and high efficiency, and is widely applicable.

The invention relates to an automatic inflow control device performance test system and method, characterized by comprising a circulatory system, a metering system, a data acquisition and processing system and a performance test system; the circulatory system provides a single-phase or multi-phase test fluid and also recycles the test fluid; the metering system is used for detecting temperature, pressure and flow data of the single-phase or multiphase fluid in the circulatory system and sends to the data acquisition and processing system; a to-be-tested automatic inflow control device is arranged in the performance test system, the performance test system is connected with a test fluid supply pipeline of the circulatory system and used for testing performance of the to-be-tested automaticinflow control device according to the single-phase or multiphase fluid provided by the circulatory system and sending test data to the data acquisition and processing system; and the data acquisitionand processing system is used for analyzing and processing data acquired by the metering system and the performance test system. The system provided by the invention can be widely applied to performance test of the automatic inflow control device.

The invention discloses an oxygen index tester which comprises an oxygen channel, a nitrogen channel, a controller module and a human-machine interface module, wherein the oxygen channel and the nitrogen channel are both connected with a gas mixing cylinder, and then connected with a combustion cylinder through a temperature controller and an oxygen sensor; the oxygen channel and the nitrogen channel are both provided with a flow switch valve and a quality flow controller; a temperature sensor is arranged in the temperature controller; the controller module is connected with a control input end of the temperature controller; the detection values of the temperature sensor in the temperature controller and the oxygen sensor are collected at the same time, and the detection values are output to the human-machine interface module; the human-machine interface module comprises a display part, an input part and a control part, wherein the input part inputs a control quantity related parameter value to the control part, and the control part respectively calculates the control quantity of oxygen and nitrogen according to the related parameter value and then outputs the control quantity to the controller module; and the controller module controls the quality flow controller according to the received control quantity so as to achieve the aim of automatically adjusting and controlling as well as improving the test precision.