The invention belongs to the technical field of
bacteria detection, and specifically discloses a method for accurately determining
bacteria in special condition on basis of microfluidic visualizationtechnology as well as selecting and enriching the
bacteria in the special condition. The method for accurately determining the bacteria in the special condition on basis of the microfluidic
visualization technology as well as selecting and enriching the bacteria in the special condition comprises the following steps: preparing bacterium fluid into microdroplets by adopting a microfluidic technology; and then, culturing the prepared microdroplets, taking photos of the cultured microdroplets, and carrying out counting by performing manual counting or in virtue of a
computer technology and the like so as to obtain the number of the bacteria in the special condition. Moreover, the method also enables selection and enrichment of the bacteria in the special condition. Compared with existing counting mode adopting plate counting, or only counting living bacteria with complete membranes, the method for accurately determining the bacteria in the special condition on basis of the microfluidic
visualization technology as well as selecting and enriching the bacteria in the special condition has greatly improved accuracy, and breaks through the existing counting mode of the bacteria in the special condition, so that
food hygiene status can be timely and effectively judged; and thus, the method is an effective means for eliminating foodborne diseases caused by recessive remains of foodbornepathogens. In addition, the method is capable of performing selection and enrichment on the bacteria in the special condition, so that result error caused by
cell heterogeneity in
research process canbe reduced; and thus, the method is of relatively great value to studies on mechanisms of
cell changes and bacteria growth activities.