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Brown algae and detection method for detecting alginic acid content of brown algae product

A detection method and technology of alginic acid, applied in the field of analytical chemistry

Active Publication Date: 2017-02-22
BEIJING LEILI MARINE BIOINDUSTRY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This detection method realizes the detection of alginic acid content through high-performance liquid chromatography, which has the advantages of high efficiency, high sensitivity, and effectively solves the problem that carbohydrates do not absorb ultraviolet rays, and can accurately detect alginic acid extracts. Qualitative and quantitative analysis, with good separation effect and wide linear range, has the advantages of strong pertinence, accuracy, high resolution, fast analysis, good selectivity, etc., which is conducive to wide application

Method used

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  • Brown algae and detection method for detecting alginic acid content of brown algae product
  • Brown algae and detection method for detecting alginic acid content of brown algae product
  • Brown algae and detection method for detecting alginic acid content of brown algae product

Examples

Experimental program
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Effect test

Embodiment 1

[0080] 1. Prepare the test solution:

[0081] (1) Hydrolysis of brown algae:

[0082] Accurately weigh 1.25g of brown algae (accurate to 0.0001g), put it in a 25ml volumetric flask, add water to dissolve it fully, make it to volume, then precisely pipette 1ml into a 20ml hydrolysis bottle (accurately divide 1ml of the liquid directly), add 1.0ml Concentrated sulfuric acid with a concentration of 98%, then hydrolyze in a water bath at 82°C for 30 minutes, take it out, cool to room temperature, add 5ml of water to dilute, and then place it in a water bath at 82°C to continue hydrolysis for 30 minutes;

[0083] (2) Derivation of the test product:

[0084] Take out the hydrolyzate of step (1) and place it in an ice-water bath to cool, then neutralize it with 8mol / L sodium hydroxide solution, adjust the pH value to neutral, then transfer to a 25ml volumetric flask to constant volume, take 1ml of the solution in a 10ml volumetric flask Add 1ml of 0.3mol / L sodium hydroxide solution...

Embodiment 2

[0128] 1. Prepare the test solution:

[0129] (1) Hydrolysis of brown algae:

[0130] Accurately weigh 1.25g of brown algae (accurate to 0.0001g), put it in a 25ml volumetric flask, add water to dissolve it fully, make it to volume, then precisely pipette 1ml into a 20ml hydrolysis bottle (accurately divide 1ml of the liquid directly), add 0.5ml Concentrated sulfuric acid with a concentration of 90%, then hydrolyze in a 75°C water bath for 60 minutes, take it out, cool to room temperature, add 5ml of water to dilute, and then place it in a 75°C water bath to continue hydrolysis for 60 minutes;

[0131] (2) Derivation of the test product:

[0132] Take out the hydrolyzate of step (1) and place it in an ice-water bath to cool, then neutralize it with 8mol / L sodium hydroxide solution, adjust the pH value to neutral, then transfer to a 25ml volumetric flask to constant volume, take 1ml of the solution in a 10ml volumetric flask Add 0.5ml of 0.2mol / L sodium hydroxide solution and...

Embodiment 3

[0151] 1. Prepare the test solution:

[0152] (1) Hydrolysis of brown algae:

[0153] Accurately weigh 1.25g of brown algae (accurate to 0.0001g), put it in a 25ml volumetric flask, add water to dissolve it fully, make it to volume, then precisely pipette 1ml into a 20ml hydrolysis bottle (accurately divide 1ml of the liquid directly), add 1.0ml Concentrated sulfuric acid with a concentration of 95%, then hydrolyze in a 90°C water bath for 90 minutes, take it out, cool to room temperature, add 5ml of water to dilute, and then place it in a 90°C water bath to continue hydrolysis for 90 minutes;

[0154] (2) Derivation of the test product:

[0155] Take out the hydrolyzate of step (1) and place it in an ice-water bath to cool, then neutralize it with 8mol / L sodium hydroxide solution, adjust the pH value to neutral, then transfer to a 25ml volumetric flask to constant volume, take 1ml of the solution in a 10ml volumetric flask Add 5ml of 2mol / L sodium hydroxide solution and 5ml...

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Abstract

The invention provides a brown algae and a detection method for detecting the alginic acid content of a brown algae product. According to the technical scheme of the detection method, the content of alginic acid in the brown algae is measured through high performance liquid chromatography. The condition of high performance liquid chromatography is as follows: octadecylsilane chemically bonded silica is adopted as the filler of a C18 chromatographic column and an acetonitrile-monopotassium phosphate buffer solution is adopted as a mobile phase. The detection method realizes the content detection of alginic acid through high performance liquid chromatography. The high performance liquid chromatography is high in efficiency, fast in speed and high in sensitivity. Meanwhile, the problem that the ultraviolet absorption is invalid for the saccharide material can be effectively solved. Moreover, the qualitative and quantitative analysis for an alginic acid extract can be accurately realized. In addition, the advantages of good separation effect, wide linear range, strong pertinence, good accuracy, high degree of separation, fast analysis, good selectivity and the like are realized. Therefore, the method can be widely applied.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for detecting the content of alginic acid in brown algae and products using high-performance liquid chromatography. Background technique [0002] Alginic acid is a carbohydrate extracted from brown algae, which is the cell wall filler of brown algae. In terms of composition, alginic acid is a heterogeneous long-chain polymer formed by the polymerization of two uronic acids, D-mannuronic acid and L-guluronic acid. Generally speaking, the biosynthesized alginic acid is basically similar in structure, but the D-mannuronic acid and L-guluronic acid of alginic acid will be significantly different depending on the type of seaweed, the ecological environment and the season, and eventually cause algal Acid properties vary. [0003] According to relevant reports, alginic acid can be used as a stabilizer and thickener in the food industry such as ice cream, ice milk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062G01N2030/146
Inventor 汤洁严国富
Owner BEIJING LEILI MARINE BIOINDUSTRY INC
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