48results about How to "Rapid detection means" patented technology

The invention relates to a method for automatic identification and detection of defects in a composite material. The method comprises steps of: detecting the composite material to generate an infrared image by using infrared thermal wave nondestructive testing equipment; conducting phase space reconstruction on the infrared sequence image to determine defect position of the composite material and segment defect area of the image; conducting phase space reconstruction on the infrared sequence image with defect area and carrying out singular value decomposition to obtain a singular matrix, and left and right projection matrixes; carrying out matrix reconstruction again on the two projection matrixes; extracting algebraic characteristics of time information and space information of the defect through singular value decomposition; constructing mixing characteristic vector as characteristic symptom of the defect; and utilizing results from a nerve network classifier to complete the identification and classification determination. The method of the invention can realize automatic identification and detection on defect in the composite material, carry out rapid detection on damage type of the composite material and provide rapid detection means according to usage condition of the composite material, and has critical reality meaning and research value.

The invention discloses a UPLC-MS / MS simultaneous flux detection method for multiclass veterinary drug residue in raw fresh milk. The method includes the steps of: (1) extracting a veterinary drug compound in a to-be-detected sample to obtain an extracted solution; (2) concentrating the extracted solution, and then regulating the pH to 7.5-10.0 to obtain a column passing solution; (3) activating a solid phase extraction column; and (4) loading the column passing solution on the activated solid phase extraction column, leaching the solid phase extraction column with leacheate, then conducting elution with eluent, collecting the eluent, carrying out concentration, redissolving and filtration by a filter membrane, then performing qualitative and quantitative determination by UPLC-MS / MS. The method provided by the invention can simultaneously detect 38 veterinary drug residue in raw fresh milk, and has the characteristics of high sensitivity and low detection limit. The matrix standard curve correlation degree, the standard addition recovery rate of the method and the intra-day and inter-day precision are all accord with the China, European Union and international veterinary drug residue analysis method requirements. The method provided by the invention provides efficient and accurate detection means for raw fresh milk veterinary drug residue risk analysis and warning research.

The present invention provides a processing method and processing apparatus for sequencing data. The processing method comprises: acquiring nucleotide sequence information originating from a maternal peripheral blood sample by means of high throughput sequencing; dividing a reference genome into a plurality of specific regions, wherein NRSc values in the specific regions are equal; allocating nucleotide sequence information originating from all chromosomes of the maternal peripheral blood sample to the plurality of specific regions of the reference genome, and collecting statistics of an NRSc value of the sample in each specific region; modifying the NRSc value of the sample in each specific region by using a GC content, and marking a modified NRSc value as an NRSs' value; based on the NRSs' value, separately collecting statistics of mean values of NRSs' values of all specific regions on a target chromosome and a contrast chromosome, and marking the mean values as a first mean value and a second mean value; and performing a difference check on the first mean value and the second mean value, and according to a difference check result, determining whether the chromosome has aneuploidy. The processing method improves accuracy of sequencing data processing.

The invention discloses a beta2-MG chemiluminescence immunoassay kit and a preparation method therefor, belongs to the technical field of the immunoassay, and solves the problems of low technique sensitivity and high cost of the prior art. The kit comprises the following reagents: R1: the liquid containing streptavidin-coated magnetic beads; R2: the liquid containing the acridinium ester-labeled beta2-MG antibody; and R3: the liquid containing the biotin-labeled beta2-MG antibody. The beta2-MG chemiluminescence immunoassay kit of the invention has the advantages of high detection sensitivity,strong specificity, good repeatability, wide detection range and good stability, and can be widely used for clinical detection of the beta2-MG.

The invention relates to a poria cocos standard decoction UPLC characteristic spectrum construction method and a detection method. The poria cocos standard decoction UPLC characteristic spectrum construction method comprises the following steps of (1) precisely weighing poria cocos standard decoction, and preparing to obtain a poria cocos standard decoction supply test solution; and (2) analyzingthe poria cocos standard decoction supply test solution by adopting an ultra-high performance liquid chromatograph to obtain a poria cocos standard decoction UPLC characteristic spectrum. The method is relatively high in precision, stability and reproducibility, fully shows the chemical component characteristics of the poria cocos standard decoction, can comprehensively and accurately control thequality of the poria cocos standard decoction, and also provides a basis for the establishment of the poria cocos medicinal material and poria cocos formula particle quality standard.

The invention relates to a rhizoma drynariae crude product and a UPLC fingerprint spectrum construction and identification method thereof. The construction method comprises the following steps: respectively preparing a reference substance solution and a test substance solution, and detecting the reference substance solution and the test substance solution by adopting a high performance liquid chromatography, wherein the step of preparing the reference substance solution comprises the steps of taking reference substances, adding a solvent and dissolving the reference substances, and the reference substances comprise 5-hydroxymethylfurfural, protocatechuic acid, neoeriocitrin and naringin; and the step of preparing the test solution comprises the following steps: taking a rhizoma drynariae processed product, adding a solvent, extracting, filtering and collecting filtrate, wherein the rhizoma drynariae processed product is a rhizoma drynariae microwave processed product. According to the present invention, the obtained fingerprint can simultaneously contain the characteristic peaks of the rhizoma drynariae crude product and the different processed products thereof, and can completely and comprehensively reflect the chemical component information of the rhizoma drynariae crude product and the different processed products thereof, such that the fingerprint can be simultaneously used for identifying the rhizoma drynariae crude product and the various rhizoma drynariae processed products, and the application range is wide.

The invention discloses a fluorescent probe for detecting and quantifying signal molecule of Gram negative bacteria colony, and belongs to the technical field of rapid detection of pathogenic bacterium. According to the fluorescent probe, a quantum dot is connected with MIP through a surface molecular imprinting technology, and the purpose of accurate quantitative detection is achieved through a fluorescence value on the basis of high degree specificity detection and fluorescence. MIP polymer is precipitated and polymerized on the surface of an amino-silane functional group modified by a CdSe / ZnS quantum dot in advance, so that QDs@MIP is successfully synthesized. The invention further discloses a preparation method and the application of the molecular imprinting fluorescent probe. The fluorescent probe can realize indirect detection of part of Gram negative bacteria, has the advantages of sensitivity, speediness and high specificity, and is low in price, and the method has an important implication for food safety or health care facilities, and can be applied to the direct monitoring of Gram negative bacteria.

A detection method and system for alignment and stiffness of bridges and tunnels based on fiber optic gyroscope technology. In this method, the linear detection walking device moves along the inner surface of the bridge or tunnel structure under test, and the data measured by the fiber optic gyroscope and the linear velocity sensor are processed by algorithm, and the motion track of the device is calculated according to the formula, that is, the gravity of the bridge structure under test Direction and continuous deformation trajectory of tunnel strike or cross-section circumferential direction. Based on the data stability judgment and outlier elimination algorithm, the reverse correction algorithm of the position information of the reference point and the reference point, and the filtering algorithm of the overall large deformation of the structure and the local small deformation of the surface, the data is corrected and calculated to obtain the local minimum of the stiffness analysis of bridges and tunnels. Deformation culling curves. This method is used to detect the local damage of bridges and tunnel structures, and the global deformation trajectory detection of bridge vertical, tunnel direction and multi-cross sections. Compared with traditional bridge and tunnel alignment detection, this method has shorter test period, high operability, low cost, high precision and high data consistency.

The invention belongs to the field of bacterial drug-resistant molecular detection, and relates to a multiple real-time fluorescent PCR method for identifying the drug-resistant mutation of macrolides and for identifying Campylobacter jejuni. The method is characterized in that an idiocratic primer, MGB probe, and combination of both are designed, not only is the specificity identification performed for the Campylobacter jejuni, but also the mutation of nucleotide of 2074th and 2075th of the 23S rRNA gene of Campylobacter jejuni and that of nucleotide of 170th and 221st of L4 flagellum gene rp1D of ribosome protein can be detected at the same time. The quick detection for drug-resistant mutation points of the various macrolides and the specificity identify for the Campylobacter jejuni are realized, which is first realized in the same reaction system, so that the identification and drug tolerance detection for the Campylobacter jejuni in clinical trials is greatly reduced, and as a result, a novel method is provided for Campylobacter jejuni drug-resistant monitoring and medicines for clinical trials infection treatment.

The invention discloses the application of neuraminic acid and neuraminidase inhibitor in chronic heart failure, and belongs to the field of diagnosis, prevention and treatment of cardiovascular diseases. The invention discovers a new biological marker-neuraminic acid, And confirmed the role of neuraminic acid as a diagnostic, prognostic biomarker and risk stratification in chronic heart failure, as well as its role as a drug target in the prevention, remission and / or improvement of chronic heart failure; therefore, the kit was used to detect nerve The amino acid level can be used to predict and assist in the diagnosis of chronic heart failure or evaluate the prognosis of chronic heart failure, and control the level of neuraminic acid through drugs to carry out more intensive diagnosis and follow-up treatment of the disease, obtain a favorable course of disease, and clinically Has huge application value.

The invention relates to a method for constructing UPLC characteristic maps of Bran-fried Atractylodes Rhizome, standard decoction and formula granules and its identification method, comprising the following steps: (1) Accurately weighing Bran-fried Atractylodes Rhizome decoction pieces, standard decoction and formula granule powder , prepared the test solution of bran-fried Atractylodes herb decoction pieces, standard decoction and formula granules; (2) analyzed the test solution of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules by ultra-high performance liquid chromatography, and obtained Bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules UPLC characteristic chromatograms. The present invention adopts and establishes the UPLC characteristic spectrum of bran-fried Atractylodes herb decoction pieces, standard decoction and formula granules, and determines 9 common peaks, of which No. 9 peak is Atractylodes atractylodes peak; The UPLC characteristic chromatograms of decoction and formula granules provide a rapid and comprehensive detection method for the quality of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules.