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Poria cocos standard decoction UPLC characteristic spectrum construction method and detection method

A characteristic map and construction method technology, applied in the field of quality standard research of traditional Chinese medicine formula granules, can solve the problem that the triterpene components cannot be well reflected, and achieve the effect of stable method, good reproducibility and high precision

Inactive Publication Date: 2019-11-12
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the 2015 edition of "Chinese Pharmacopoeia", the quality control of Poria cocos did not involve the qualitative and quantitative determination of clear ingredients, which has certain defects in the quality control of Poria cocos medicinal materials
[0006] Since the decoction medium of Poria cocos standard decoction is water, many water-insoluble triterpene components cannot be well reflected in the standard decoction

Method used

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  • Poria cocos standard decoction UPLC characteristic spectrum construction method and detection method
  • Poria cocos standard decoction UPLC characteristic spectrum construction method and detection method
  • Poria cocos standard decoction UPLC characteristic spectrum construction method and detection method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Construction of characteristic map of Poria standard decoction

[0049] See Table 1, Table 2, Table 3, and Table 4 for the main instruments, reagents, reference substance information, and source of Poria standard decoction, respectively.

[0050] Table 1 Main Instruments

[0051]

[0052] Table 2 Main Reagents

[0053]

[0054] Table 3 Reference substance information

[0055]

[0056] Table 4 Origin information of 25 batches of Poria standard decoction

[0057]

[0058] experiment method

[0059] Preparation of reference solution

[0060] Accurately weigh an appropriate amount of pachymic acid B reference substance, add methanol to make a solution containing 20 μg of pachymic acid B per 1 ml, and obtain it.

[0061] Preparation of Poria standard decoction

[0062] Take 200g of Poria decoction slices, put them in an electric ceramic pot, add water to decoct twice, add 9 times the amount of water to the first decoction, soak for 50 minutes, boil at 500W ...

Embodiment 2

[0111] Poria standard decoction detection method, detection steps are as follows:

[0112] (1) Precisely weigh the Poria standard decoction to be tested, and prepare the sample solution of the Poria standard decoction to be tested;

[0113] (2) Precisely absorb the sample solution of standard Poria cocos decoction to be detected, inject it into an ultra-high performance liquid chromatograph, measure it, and obtain the UPLC characteristic map of standard Poria cocos decoction to be detected;

[0114] (3) Compare the measured UPLC characteristic spectrum with the constructed Poria standard decoction UPLC characteristic spectrum. If it is consistent with the UPLC characteristic spectrum of Poria standard decoction, the quality is qualified.

[0115] Chromatographic conditions

[0116] Use octadecylsilane bonded silica gel as filler (column length is 150mm, inner diameter is 2.1mm, particle size is 1.6μm); acetonitrile is used as mobile phase A, 0.1% formic acid is used as mobile...

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Abstract

The invention relates to a poria cocos standard decoction UPLC characteristic spectrum construction method and a detection method. The poria cocos standard decoction UPLC characteristic spectrum construction method comprises the following steps of (1) precisely weighing poria cocos standard decoction, and preparing to obtain a poria cocos standard decoction supply test solution; and (2) analyzingthe poria cocos standard decoction supply test solution by adopting an ultra-high performance liquid chromatograph to obtain a poria cocos standard decoction UPLC characteristic spectrum. The method is relatively high in precision, stability and reproducibility, fully shows the chemical component characteristics of the poria cocos standard decoction, can comprehensively and accurately control thequality of the poria cocos standard decoction, and also provides a basis for the establishment of the poria cocos medicinal material and poria cocos formula particle quality standard.

Description

technical field [0001] The invention belongs to the field of research on quality standards of traditional Chinese medicine formula granules, and in particular relates to a construction method and a detection method of UPLC characteristic map of Poria standard decoction. Background technique [0002] Poria cocos is the dried sclerotia of Poria cocos (Schw.) Wolf (the 2015 edition of "Chinese Pharmacopoeia"), which is a commonly used traditional Chinese medicine. Edema, oliguria, phlegm, dizziness and palpitations, spleen deficiency, lack of food, loose stools and diarrhea, restlessness, palpitations and insomnia, etc., have a wide range of clinical applications. Poria cocos is sweet, light and flat in nature and flavor, and it is listed in the "Shen Nong's Materia Medica", which is listed as the top grade. [0003] The main components contained in Poria cocos are polysaccharides and triterpenoids. In addition, it has been reported that Poria cocos also contains inorganic sub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 何广铭罗文安吴晓纯蔡盛康彭致铖黄小丹魏梅孙冬梅程学仁陈向东
Owner GUANGDONG YIFANG PHARMA
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