41results about How to "Promote expression" patented technology

The invention discloses a method and a device for online addition of lyric subtitles to pictures, relates to the technical field of photograph special effect processing for mobile electronic equipment and aims to provide a method and a device for online addition of the lyric subtitles to pictures taken by intelligent mobile terminals. The method technically includes: step 1, a client acquires editing instructions inputted by a user, wherein the editing instructions include a picture requiring subtitle addition, picture clipping parameters, a song name, a first line of lyrics and a second line of lyrics; step 2, the client uploads the editing instructions to a server; step 3, the server clips the picture requiring subtitle addition according to the picture clipping parameters; step 4, the server renders the song name, the first line of lyrics and the second line of lyrics onto the clipped picture and stores the finished picture; step 5, the server returns a storage address of the finished picture to the client; step 6, the client downloads the finished picture from the server according to the storage address and displays the finished picture to the user.

The invention belongs to the technical field of gene engineering, and provides a sesame drought-resistant gene SiSAM1 and application thereof. The sesame drought-resistant gene SiSAM1 is a nucleotidesequence as shown by SEQ ID NO.1; or a nucleotide sequence with drought-resistant performance generated by adding, substituting, inserting or omitting on or more nucleotide sequences of the nucleotidesequence as shown by SEQ ID NO.1. The SiSAM1 gene is established onto an expression vector, the SiSAM1 gene is transferred into Arabidopsis by virtue of agrobacterium-mediated conversion method to obtain transgenic agrobacterium; the transgenic agrobacterium and non-transgenic agrobacterium are simultaneously treated in a drought stress manner, and comparing the transgenic agrobacterium with thenon-transgenic agrobacterium, the expression and drought-resistant capacity of SiSAM1 can be greatly improved. By adopting the sesame drought-resistant gene SiSAM1, the drought-resistant capacity of plants can be improved, and the sesame drought-resistant gene SiSAM1 can be used for the breeding of an oil crop drought-resistant variety, so that the drought-resistant performance of crops can be improved, and the high yield and stable yield of the crops can be ensured.

The invention discloses a biological fermentation production technology of hyaluronic acid and belongs to the field of biological fermentation. The biological fermentation production technology of thehyaluronic acid, disclosed by the invention, comprises the following steps: activating strains, culturing a seed solution, carrying out amplified culture on the strains and carrying out fermentationculture. The fermentation culture is divided into a fermentation earlier stage and a fermentation later stage; the fermentation earlier stage and the fermentation later stage are applicable to self-growth of thalli and accumulation of the hyaluronic acid respectively. According to the production technology disclosed by the invention, a self-growth process of the thalli and a product expression process are separated relatively to realize the balance of self-metabolism of the thalli and product expression, so that the expression of the hyaluronic acid is promoted; nutrient components in a fermentation process are improved so that the growth of the thalli and the accumulation of the hyaluronic acid can be promoted better; a class index material supplementing manner is used for fermenting according to a growth principle of the thalli, so that the yield of the hyaluronic acid is improved.

Provided is an anti-angiogenic fusion protein, which comprises a vascular endothelial cell growth inhibitor (VEGI) or variant thereof, and other polypeptide such as IgG Fc. The fusion protein optionally comprises a linker. The fusion protein can induce the apoptosis of endothelial cells and inhibit the growtth of endothelial cells, so as to be used for treating tumor. Also provided are a pharmaceutical composition and use of the fusion protein.

In certain aspects, the present invention provides compositions and methods for increasing thermogenic adipocytes (e.g., brown adipocytes or other UCP-1 expressing adipocytes) by administering an antagonist of an ActRIIB signaling pathway. Examples of such antagonists include ActRIIB polypeptides, anti-ActRIIB antibodies, anti-myostatin antibodies, anti-GDF3 antibodies, anti-Nodal, anti-activin, and anti-GDF11 antibodies. A variety of metabolic and other disorders may be treated by causing an increase in thermogenic adipocytes.

The invention discloses a making method of an aggrecanases inhibitor combined with in-vitro cross-linked hyaluronic acid hydrogel and application thereof in drugs for treating osteoarthritis. The making method comprises the steps of aldehyde group modification on hyaluronic acid, adipic dihydrazide modification on hyaluronic acid and in-vitro cross-linking of hyaluronic acid. The aggrecanases inhibitor combined with in-vitro cross-linked hyaluronic acid hydrogel provided by the invention has properties of sustained releasing drugs, can effectively alleviate rat osteoarthritis, promote expression of proteoglycan of osteoarthritis knees and inhibit expression of degradation products of proteoglycan. According to the making method of the aggrecanases inhibitor combined with the in-vitro cross-linked hyaluronic acid hydrogel and application thereof in drugs for treating osteoarthritis, biological materials and drugs are combined, a tissue engineering method is adopted for treating osteoarthritis, the manner of delivering drugs to articular cavities is utilized, wound caused by treatment of osteoarthritis is reduced, the sustained release drug delivery manner can improve local drug concentration while reducing corresponding side effect caused by systemic administration of drugs.

The invention discloses a method for increasing output of insulin precursors, and belongs to the technical field of biological engineering. The method comprises the following steps of connecting an artificially synthesized insulin precursor and a pichia pastoris expression carrier pPIC9K by dual-enzyme digestion through a molecular biological method, so as to build a recombination strain; performing G418 resistance screening, so as to obtain high-copying recombination type insulin precursor-containing engineering bacteria P.pastoris GS115-PI (CL012), and coexpressing SNC2 and Sso2 genes in the recombination strain. The method has the advantages that by utilizing high-density fermentation, the output of the insulin precursor of the strain is 78mg / L, and compared with the strain CL012, the output is increased by 47%.

The invention discloses application of a cucumber CsWRKY50 gene to strengthening the downy mildew resistance of the cucumber, the specific application is to overexpress the cucumber CsWRKY50 gene in acucumber plant, and the nucleotide sequence of the cucumber CsWRKY50 gene is shown as in SEQ ID:1. That overexpressing the CsWRKY50 in the cucumber can obviously improve the resistance of the cucumber to the downy mildew is found for the first time, the experiment proves that the cucumber CsWRKY50 gene is transferred into the cucumber plant as a target gene, the expression of the resistance geneis increased by changing the expression quantity of the cucumber CsWRKY50 gene in the cucumber plant, the resistance of the cucumber to the downy mildew is improved, the character is used in the breeding of the cucumber, the cucumber variety capable of resisting the downy mildew is bred by choosing the cucumber CsWRKY50 gene overexpressed plant through the gene detection method, the application method is safe and reliable, and the application has an excellent application prospect in the researches of the resistant variety of the cucumber.

A medicament for prophylactic and / or therapeutic treatment of a dermatosis resulting from excessively advanced keratinization, such as non-hereditary inflammatory keratosis and hereditary congenital keratosis, which comprises a substance selected from the group consisting of a cannabinoid agonist and a vanilloid agonist as an active ingredient.

The invention relates to sturgeon disease-resistant immune protein and a preparation method and application thereof. The sturgeon disease-resistant immune protein is sturgeon interleukin-6 in an aminoacid sequence shown as SEQ ID NO.1. Expression of the novel sturgeon interleukin-6 is induced by various immune stimulations, and functions of immune tissue cell proliferation promotion, immune molecule expression regulation and bacterial infection inhibition are achieved. The sturgeon interleukin-6 can be used as a disease-resistant preparation such as an immunity enhancement agent to improve sturgeon immunity and effectively prevent and control sturgeon disease damages, and accordingly development of the sturgeon aquaculture industry is promoted.

The invention discloses a SUMO (small ubiquitin-related modifier) and SUMO protease encoding gene, encoding protein and application thereof. The invention relates to a Saccharomyces cerevisiae gene sumo (S), which is defined by the nucleotide sequence of SEQ ID No.1 in the sequence table. The encoding protein of the Saccharomyces cerevisiae gene sumo (S) is defined by the amino acid sequence of SEQ ID No.2 in the sequence table. The SUMO protease gene ulp (S) provided by the invention is defined by the nucleotide sequence of SEQ ID No.3 in the sequence table. The encoding protein of SUMO protease gene ulp (S) provided by the invention is defined by the amino acid sequence of SEQ ID No.4 in the sequence table. The invention can realize high expression of Saccharomyces cerevisiae sumo gene and SUMO protease ulp gene in Escherichia coli, specifically realize high expression in the commonly used expression strain BL21(DE3) of Escherichia coli, and ensures that the function is not affected.

The invention belongs to the field of medicines and in particular relates to a novel medical application of quercetin and derivatives thereof, that is, quercetin and derivatives have the action of treating cholestasis. Tests verify that the quercetin compounds reduce concentration of toxic cholate CDCA and TCDCA in blood serum and liver tissues and increase concentration thereof in bile, kidney tissues and urine by up-regulating expression of cholestasis rat liver transporters Ntcp, Bsep, Mrp2 and Mrp4 and kidney transporters Mrp4 and Oat4 as well as a liver nuclear receptor FXR, thereby playing a role of treating cholestasis.

The invention provides a callus-specific promoter cloned from rice, an expression vector containing the promoter and a selective marker gene optimized by a rice codon, and a transformant. A nucleotide sequence of the promoter is shown in SEQ ID NO.1; and the promoter has the expression specificity of calli, and has higher activity in the calli. The expression of the selective marker gene (a hygromycin B phosphotransferase gene with a sequence shown in SEQ ID No.2) optimized by the codon in a normal rice plant is controlled by using the promoter, so that the transgenic rice can be efficiently screened, and the problem of the safety of the selective marker gene to the biological environment can be completely solved; therefore, the biological safety of the transgenic rice is improved, and the selection efficiency is improved at the same time.

The invention discloses high-nucleic-acid baker's yeast and a preparation method thereof. Compared with 100% weight of baker's yeast thalli, the high-nucleic-acid baker's yeast contains 21wt% or aboveof nucleic acid, wherein the weight of RNA is greater than 13%. Yeast is subjected to activation and culture, and the fermentation, growth and proliferation activity of the yeast are promoted throughliquid mediums having high-concentration glucose, so that the expression of RNA substances in microzyme cells is promoted, and increase of the whole nucleic acid content of primary-generation microzyme cells is facilitated; and then through low-speed centrifugal separation, microzyme containing more nucleic acid components and larger in unicellular weight is separated, so that the purity of the high-nucleic-acid yeast is further improved, filial-generation yeast cells obtained through subsequent enlarged culture contain high nucleic acid content, and the nucleic acid content of the produced microzyme is increased wholly.

The invention provides a hematopoietic stem cell. The hematopoietic stem cell is reduced in ZBTB7A gene expression amount or free from ZBTB7A gene expression amount. According to the invention, a CRISPR technique, when used for knocking out a fetal hemoglobin gene inhibiting factor, is simple and convenient to operate and short in time consumption; after the fetal hemoglobin gene inhibiting factor, namely ZBTB7A, is knocked out, influence to normal functions of nucleated red blood cells is avoided, and treatment means for thalassemia are widened; and by taking the autologous hematopoietic stemcell of the patient as a transplant, transplantation immunological rejection can be completely avoided and risks of stem cell treatment can be reduced.

This invention discloses a protein coded by rice Osnaat1-1 gene, which has the amino acid sequence as shown in SEQ ID No.2. The rice Osnaat1-1 gene has the nucleotide sequence as shown in SEQ ID No.1. This invention also discloses a method for modifying rice. The method comprises: modifying rice by conventional gene mutation technique with Osnaat1-1 gene sequence to obtain rice Osnaat1-1 mutant as shown in SEQ ID No.1. The mutant is rich in Fe and Zn.

The invention relates to the field of biotechnologies, and in particular relates to a pluripotent stem cell as well as a preparation method and an application thereof. The pluripotent stem cell is a mesenchymal stem cell carrying a Betatrophin protein coding gene, shows typical characteristics of mesenchymal cells, can induce a transcription factor of a co-cultured islet cell for stably expressing beta cell specificity so as to improve the multiplication capacity of the islet cell and enhance the reaction, of the islet cell, for insulin secretion in response to excitation irritation of kcl and Arg; and meanwhile, the pluripotent stem cell is discovered to be capable of inhibiting inflammation and expression of an apoptosis factor, and increasing the expression of anti-inflammatory factors. The pluripotent stem cell, transplanted into an STZ-induced type-1 diabetic mouse model, has a significant treatment effect on a diabetic mouse.

The invention provides a human and mammal cell expression vector and application thereof. A nucleotide sequence of the expression vector is shown in Seq ID. No.1 or Seq ID No.2; the expression vector is the expression vector built by taking pCAT3-control as a starting vector. By adopting the expression vector disclosed by the invention, the expression level of the carried target gene can be significantly improved, and compared with the expression vector which contains MAR (matrix attachment region) and does not contain EGFP (enhanced green fluorescence protein), expression of the target gene can be significantly improved under the same conditions.

The invention discloses a human epidermal growth factor hEGF gene optimization sequence, a method for preparing the same and application of the human epidermal growth factor hEGF gene optimization sequence. The human epidermal growth factor hEGF gene optimization sequence is shown as SEQIDNO.1. The human epidermal growth factor hEGF gene optimization sequence, the method and the application have the advantages that cDNA [complementary DNA (deoxyribonucleic acid)] structures of EGF (epidermal growth factor) genes are changed, alkali bases of individual genes on nucleotide sequences are replaced by synonym codons, genes with the optimal codon usage bias are selected to synthesize EGF optimization sequences, prokaryotic expression vectors pET-30a-hEGF are constructed and can be expressed in Escherichia coli hosts in a high-level manner, and accordingly the human epidermal growth factor hEGF gene optimization sequence, the method and the application have important significance on EGF industrial production and application.

The invention discloses a DC cell culture method, a culture medium, a DC treatment strategy-based drug and application of a tyrosine kinase inhibitor in preparation of the DC cell culture medium. According to the DC cell culture method, in the culture process of induced differentiation of immature DC cells, a tyrosine kinase inhibitor is added into a culture medium, and the mature DC cells are obtained through induced differentiation culture; the culture medium is a cell culture medium containing a tyrosine kinase inhibitor and can be used for culturing immature DC cells which are induced to be differentiated into mature DC cells; the medicine based on the DC treatment strategy contains a tyrosine kinase inhibitor; and the tyrosine kinase inhibitor can be used in the preparation of the DC treatment strategy-based medicine. According to the scheme, the problem that an existing DC cell culture method cannot meet the requirement for highly mature or high-activity DC cells required by clinical curative effects can be solved, and the aim of improving the maturity or activity of the DC cells is achieved.

The invention relates to the technical field of gene engineering, in particular to a chlorella vulgaris chloroplast homologous recombinant empty vector and application thereof. The recombinant empty vector comprises upstream and downstream homologous arms, at least one promoter and at least one terminator are arranged between the upstream and downstream homologous arms, and a base sequence which is as shown in SEQ ID NO: 7 and forms a polycistron structure with at least one exogenous gene is inserted between the promoter and the terminator; and the upstream homologous arm contains a base sequence shown as SEQ ID NO: 1, and the downstream homologous arm contains a base sequence shown as SEQ ID NO: 2. By adopting a chlorella vulgaris chloroplast stable expression system disclosed by the invention, stable expression of a plurality of exogenous genes in chloroplast can be realized.

The invention discloses a populus deltoidesx populus nigra resistance-related gene PdHSP70 which plays an important role in the plant salt resistance process. The provided HSP gene is named as PdHSP70, and the gene has a base sequence shown in SEQ ID NO:3 of a sequence table. When the resistance-related gene PdHSP70 is constructed to an expression vector pCAMBIA1304, a promoter is added in front of a transcription initiation nucleotide, a selective marker GFP is added to authenticate and screen transgenosis vegetable cells or plants, and the expression vector carrying the resistance-related gene PdHSP70 can convert plant hosts through multiple methods and is used for cultivating salt-resistant plant varieties. The gene has wide application prospects in cultivation of salt-resistance plants.

A method for promoting expression of calreticulin in at least one kind of eukaryotic cell, and a synthetic peptide useful in this method are provided. In the method provided by the present invention, a culture of target cells is prepared, and a calreticulin expression-promoting peptide having calreticulin expression-promoting activity is supplied at least once to that culture.

The invention discloses application of glaucocalyxin A in preparation of a drug for treating lung cancer. Particularly, the glaucocalyxin A acts on lung cancer cells, cell apoptosis can be induced bydestroying mitochondrial membrane potential, causing Cyto-C outflow, inhibiting anti-apoptosis protein Bcl-2, promoting pro-apoptotic protein Bax expression and causing Caspase cascade reactions, anda choice of a new treatment drug is provided for lung cancer treatment.

The invention provides a construction method of a genetically engineered bacterium with high yield of squalene, an MVA pathway rate-limiting enzyme-truncated HMG-CoA reductase encoding gene (tHMG1) is integrated and expressed, and an FPP synthase encoding gene (ERG20) and a saccharomyces cerevisiae endogenous squalene synthase encoding gene (ERG9) is integrated and expressed in saccharomyces cerevisiae in a homologous recombination manner; the MVA pathway metabolism intensity is enhanced, and the squalene expression is enhanced. Meanwhile, a squalene monooxygenase encoding gene ERG1 promoter is replaced with a copper ion induced promoter pCUP1, the expression level of the squalene monooxygenase encoding gene ERG1 promoter is lowered, ergosterol synthesized by squalene epoxidation is reduced, and the squalene yield is increased; and finally the genetically engineered strain with high squalene yield can be obtained. The shake flask fermentation yield of squalene of the genetically engineered bacterium can reach about 57mg / L, the fermentation tank yield can reach about 7g / L, and the genetically engineered bacterium completely has a commercial production level and has a good industrial application prospect.

The invention provides a composition for targeted gene regulation to correct white hair and application thereof, and relates to the technical field of white hair treatment. The composition comprises the following components by weight: 0.5-6 parts of betaine extract, 0.2-4 parts of flower bean extract, 1-8 parts of carrot extract, 0.5-5 parts of oyster peptide, 0.1-2 parts of alpha-linolenic acid and 0.1-0.8 part of mineral element supplementing agent. Mineral element supplementing agent includes Cu2+, Fe2+, Zn2+, Mg2+ and Se2+. According to the composition and the application, the activity inhibition of toxic molecules on key genes CAT, KROX20, MITF, IRF4, CYP450 and hair follicle stem cells on white hair early birth or white hair generation of the aged is relieved to promote melanin deposition and turn the white hair to black hair. As shown in the embodiment, the white hair of white hair volunteers of all ages taking the composition is improved in different degrees.

The invention discloses an induction and amplification method of CIK2 (NK NK-T) cells. The method comprises the steps of induction and activation of immune cells CIK2 (NK NK-T) and large-capacity culture and amplification of the immune cells CIK2 (NK NK-T). The method also comprises a step of detecting the immune cell CIK2 (NK NK-T). According to the induced amplification method of the CIK2 (NK NK-T) cells, the percentage of CD16<+> / 56<+> CD3 <-> cells (NK cells) and CD16<+> / 56 <+> CD3<+> cells (NK-like T cells) can be stably increased, and the total number of cells cultured and amplified by the cells can be stably increased. The immune cell provided by the invention retains the characteristic that the NK cell has stronger efficacy of killing tumor cells and virus infected cells than the CIK1 cell; the low amplification rate of the NK cells is compensated, and the new immune cells CIK2 are also named as NK NK-T cells.

The invention discloses a method for preparing a soluble HIV-1 integrase recombinant protein, and belongs to the technical field of biology. The preparation method comprises the steps of induced expression, bacteria disruption, purification and the like. The adopted expression medium contains 15 to 35 g / L of tryptone, 10 to 20 g / L of yeast extract, 2 to 3 g / L of sodium chloride, 1 g / L of glucose, 10 to 15 g / L of glycerin, 2.05 g / L of disodium hydrogen phosphate, 1.27 g / L of sodium dihydrogen phosphate, 50 mg / L of magnesium sulfate, and 60 mg / L of kanamycin; and the adopted cracking buffer solution contains 20 mM of 4-hydroxyethyl piperazine ethanesulfonic acid, 1 M of sodium chloride, 2 mM of beta-mercaptoethanol, 0.3 mg / ml of lysozyme and 5 mM of imidazole, and the pH value of the buffer solution is 9.0. The expression medium can ensure normal growth of host bacteria and efficient expression of the objective protein; and the cracking buffer solution can enhance the disruption effect of bacteria, improve the solubility of the objective protein and finally improve the yield of the objective protein.

The invention relates to the technical field of skin care products, in particular to a preparation method and application of glycosylglycerol. Glyceroglucoside in the skin care product is prepared by a biological enzyme catalysis method. According to the invention, high-yield and high-purity glycosylglycerol is prepared through a biological enzyme catalysis process, and AQP3 expression can be obviously promoted; as a natural cell activator, the natural cell activator can effectively activate the regeneration capacity of skin, promote cell viability and metabolism, activate and regenerate skin cells, enhance the oxidation resistance of the skin cells, increase the moisture retention, elasticity and smoothness of the skin, relieve skin redness, resist erythema and accelerate wound healing and tissue repair. Meanwhile, the skin care product is stable in property, high in temperature resistance, high in storage stability and wide in application prospect.

The present invention provides compositions useful for transfecting cells (e.g., liver cells) to express ABCA1. The compositions described herein comprise a pharmaceutically acceptable aqueous carrier containing sonochemically-active microspheres together with a plasmid DNA construct encoding an active form of ABCA1 and at least one promoter for the expression thereof. Preferably, the sonochemically-active microspheres comprise, consist essentially of, or consist of gas bubbles (e.g., a fluorocarbon gas, such as octafluoropropane) encapsulated within protein-containing or lipid-containing shells (e.g., human serum albumin shells). The microspheres are disruptable by exposure to ultrasonic acoustic energy to release the encapsulated gas.