38results about How to "Mild fermentation conditions" patented technology

The invention discloses a bioanalysis preparation technique of a biological organic fertilizer by chicken manure. High-efficient and green chicken mature fermenting biological organic fertilizer is developed through a mixed bacteria aerobiotic two-step fermenting technique, wherein an one-step fermenting flora comprises aspergillus, thermophilic sporotrichum, saccharomycetes, bacillus subtilis, thermophilic heterotrophism ammonia oxidizing bacteria; a two-step aerobiotic fermenting flora comprises EM fungicide, azotobacter chroococcum, and bacillus licheniformis. The technique adopts multi-bacterium mixing fermentation; through combination of metabolic capabilities, the complex metabolism which is hard to complete by single culture is completed; through synergic fermentation between microorganisms, the chicken mature is effectively transformed, the hygienic index reaches the national standard, and the product decomposition and growth promoting index are significantly promoted. The fermenting technique is simple and high-efficient, the deodorization and sterilizing effects are good; besides, the production cost is low, and the technique is easy to carry out industrial scale production.

The invention belongs to the technical field of tobacco processing and relates to a method for preparing basic material of a tobacco thin sheet, namely tobacco stem cellulose through the microbial solid fermentation process. Microbes including high-yield prolease, pectolase, ligninase, amylase and other enzymes are selected, and tobacco stems, tobacco smalls and crumbled tobacco are taken as raw materials for preparing microbial seeds, the prepared microbial seeds are inoculated into the tobacco stems for performing solid fermentation, soaking the tobacco stems after fermentation in buffer solution, further performing enzymolysis for degrading the tobacco stems after the enzymolysis, proteins, lignin, pectic substances and other macromolecular substances into micromolecular substances which are easy to be separated from cellulose type substances in the tobacco stems, and further stirring, pulping, filtering and separating for getting water-soluble extract and water-insoluble celluloseof the tobacco stems. The method has the advantages of being simple in process, saving energy, reducing equipment investment and improving the synthetic quality of the tobacco thin sheet, and can greatly reduce the residual quantity of the proteins, pectin, starch and other macromolecules in the tobacco thin sheet.

The invention discloses a fermentation and optimization method for tobacco flavors, which takes tobacco residual materials as raw materials, such as tobacco stems, tobacco dusts and secondary tobacco leaves, the tobacco residual materials are put into a fermentor, sugar and inorganic salt, tobacco flavor bottom materials, and water are added in to regulate pH value and a fermentation medium is obtained, and well-known preserving strain, micrococcus luteus ACCC11001, is selected as liquid fermentation strain and is fermented under certain fermentation conditions. The tobacco flavors after fermentation culture not only can maintain characteristic fragrance contained in original flavors, but also can ensure auxiliary fragrance obtained by fermentation to combine perfectly with the characteristic fragrance, and compared with the original flavors, the grade of the fragrance is improved obviously and the fragrance smells more elegant. The production process of the invention is simple, convenient and easy for extension, thus being provided with greater development and application value.

The invention discloses a novel process for processing chicken manure through aerobic fermentation. A high-efficiency, green chicken manure fermented bio-organic fertilizer is developed by using a rapid fermentation process of mixed bacteria solids. The compound microbial agents include bacillus coagulans, bacillus licheniformis, thermophilic heterotrophic ammonia-oxidizing bacteria, aspergillus niger and the like. The process adopts multi-bacterium mixed fermentation, through the combination of different metabolic abilities, the complex metabolic effects that are difficult to complete by a single strain can be completed, through the synergistic fermentation between microorganisms, the effective conversion of chicken manure is realized, the health indexes meet the national standards, and the product maturity and growth promotion indexes are significantly improved. The fermentation process is simple and efficient, has good deodorizing and sterilizing effect, has low production cost, andis easy for industrial large-scale production.

The invention discloses a production method for a vinasse bio-organic fertilizer, and the method belongs to the field of comprehensive utilization of liquor-making byproducts. According to the production method, vinasse, corn straw, weed, melon vine, peanut shell, sawdust, starch residue, mildewed feed, chicken and duck manure, organic-fertilizer ferment, a deodorant, biological azotobacter, fiber degrading bacteria, organic-phosphorus-dissolving bacteria, ammonifier, phosphate-solubilizing bacteria, potassium-solubilizing bacteria and a phosphorus activator are taken as raw materials, and processes of crushing and fermentation of organic raw materials, fermentation degradation of chicken and duck manure, dewatering, pelleting, drying and the like are performed, so that a finished product is prepared. After being applied to soil, the product vinasse bio-organic fertilizer is capable of effectively improving soil hardening caused of employing of chemical fertilizers, improving physical and chemical properties of soil, improving soil porosity and permeability and exchanging property, and increasing beneficial becteria and soil microbial populations. After being applied in successive years, the vinasse bio-organic fertilizer is capable of promoting crop growth, reducing disease index of continuous-cropped crops and substantially mitigating successive cropping obstacle. The vinasse bio-organic fertilizer is a novel environment-friendly fertilizer.

The invention discloses a tremella fuciformis strain which is screened out. The tremella fuciformis strain is named as XY and is preserved in China General Microbiological Culture Collection Center, apreservation date is April 25, 2019, and a preservation number is CGMCC No.17675. The invention further provides application of the tremella fuciformis strain in tremella polysaccharide production. Amain process of using the tremella fuciformis strain for producing tremella polysaccharides includes the steps: a seed solution prepared from CGMCC No.17675 is used for inoculating a solid-state fermentation culture medium, and fermentation is conducted to obtain a fermentation broth. High-molecular-weight polysaccharides with the molecular weight of 1000-1500 kDa and low-molecular-weight polysaccharides with the molecular weight of 100-300 kDa can be prepared, a total yield can achieve 15-25 g / L, the protein content is smaller than 0.3%, compared with a liquid-state fermentation method, theprocess of using the tremella fuciformis strain for producing the tremella polysaccharides has significant cost advantages, and therefore the tremella fuciformis strain has good application prospectsin industrial production of the tremella polysaccharides.

The invention relates to the technical field of health drinks and in particular relates to a method for making plum fruit wine. The method comprises steps of pretreatment, enzymolysis, fermentation, sterilization, blending and aging. By adopting the method provided by the invention, the plum fruit wine is relatively natural and has clean and elegant fruit and wine fragrance; due to enzyme deactivation treatment and sterilization treatment, effects of rotting prevention, sterilization and oxidation prevention are effectively achieved in the making process; in the making process, yeast and infectious microbes are effectively inhibited and terminated, so that the plum fruit wine made by using the method is relatively safe and sanitary and high in quality.

The invention discloses a preparation method of composite microbial fertilizer and prepared composite microbial fertilizer. The preparation method comprises a yeast pre-fermentation step, a compositestrain fermentation step and a post-fermentation treatment step. In the invention, a straw composition is creatively pre-fermented with yeast, and metabolite contained in a fermentation product is suitable for fermentation of a composite flora. The composite microbial fertilizer has wide raw material sources, mild fermentation conditions and strong vigorous species, and after long-term use, the soil micro-ecological environment can be significantly improved, the soil fertility is improved, and the purposes of increasing crop yield and increasing efficiency are achieved.

The invention relates to the field of wine making, in particular to a making method of red wine. The novel wine which is mellower and weak in irritation is mixed through the processes of picking, crushing, sulfur dioxide introducing, pectinase adding, sugar adding, fermenting, aging and the like by reasonably matching two kinds of grapes with the different mouthfeel and the different fragrances, and by scientifically and reasonably designing the whole process steps, the made wine is mellower in mouthfeel, higher in quality and stability, not prone to deterioration and mellower in mouthfeel and has the obvious layering sensation.

The invention discloses a method for preparing ellagic acid by solid fermentation with granatum as a raw material and belongs to the field of medicines and health-care products. In the method, solid fermentation is performed by using a culture medium which takes granatum as a main ingredient and by using Aspergillusniger 3.316 as a fermenting bacterial strain. The conditions for the solid fermentation of the granatum include: the pH value is between 5 and 7; and the granatum is initially moisturized by water by 47 to 60 percent, 0.5-percent NH4NO3 is added into a moisturizing agent as an N source, and fermentation is performed at 30 DEG C for 5 to 7 days. The fermented product is purified and dehydrated to produce the high-quality ellagic acid product. The granatum serving as a raw material is cheap, and the high yield and purity of the ellagic acid product are ensured; and the method has an outstanding cost advantage and is suitable for large-scale production.

The invention discloses ellagic acid prepared from solid-state fermented pomegranate peel and a preparation method thereof, and belongs to the technical field of cosmetics, medicines and healthcare products. A shallow plastic slot is used for manufacturing a fermentation bed, loading and unloading are convenient, and multi-layer stacking can be realized; aspergillus niger and Escherichia coli are mixed and fermented to form complementary effect, so that ellagic acid conversion efficiency is higher; the ellagic acid is extracted with progressively decreased dose of absolute methanol through three times, methanol is recycled, filter slag is washed with deionized water, and freeze-drying is performed to obtain a product with good quality; and the ellagic acid product is high in yield and purity. The process steps are simple, fermentation conditions are gentle, consumption of chemical substances is greatly reduced, environmental pollution is small, and large-scale production is adapted better.

The invention relates to a compound strain-fermented goose feed and a preparation method thereof. The invention aims to provides the compound strain-fermented goose feed and the preparation method thereof to solve the problems that the original microbial flora balance state in the intestinal tract of an animal is broken and the immunity of the animal is damaged. The feed specifically comprises fermented soybean meal, silkworm chrysalis meal, plasma protein powder, earthworm powder or shrimp shell powder, a green feed, fermented astragalus residues, bentonite, stone powder, choline chloride, calcium hydrogen phosphate, sodium selenite, a vitamin B group, blueberry pomace, fermented whole-plant corn silage and fine river sand. The method specifically comprises the following steps: preparing the fermented soybean meal, the fermented astragalus residues, the green feed, the blueberry pomace, the plasma protein powder and the silkworm chrysalis meal, conducting mixing and the like. The method is applied to the field of preparation of goose feeds.

The invention provides a method for biotransformation of corn stigma with penicillium to obtain flavone. The method utilizes flavones-producing penicillium to ferment a culture solution containing corn stigma, in the fermentation process, the flavone precusor substance in corn stigma is fully utilized for biotransformation to acquire a large amount of accumulated flavone substance, and then purification is carried out to obtain high purity flavone. Specifically, the fermentation medium consists of: 30-50g of corn stigma powder, 2-5g of yeast powder, 0.5-1g of dipotassium hydrogen phosphate, 0.5-1g of magnesium sulfate, 0.5-1g of potassium chloride, 0.01-0.1g of ferrous sulfate, 0.05-0.2g of vitamin B2, 0.05-0.1g of vitamin B6, 0.05-0.3g of vitamin C, 20-40g of maltose, and the balance water added to 1L. The method provided by the invention has the advantages of low cost, high yield, high product purity and good activity, and simple operation steps, and is suitable for industrial production.

The invention provides a germ bran fermented feed and a preparation method. Raw materials of the feed contain germ bran powder, apple pomace, cottonseed meal, soybean meal, zymocyte liquid, a fermentation auxiliary agent and tapioca, wherein the germ bran powder is a mixture of at least two kinds of shiitake mushroom germ bran powder, needle mushroom germ bran powder, agrocybe cylindracea germ bran powder and oyster mushroom germ bran powder. According to the germ bran fermented feed, components in the raw materials are in combined action, and the raw materials are reasonable in compatibility.Nutrient components of the feed completely meet requirements of lactation sows for feeds. The feed is simple in preparation method, the fermentation condition is mild, the fermentation time is short,and the production cost is low.

The invention relates to a method for preparing cycloastragenol through microorganism mixed fermentation, transformation and degradation of astragaloside iv. Two strains of fusarium proliferatum 0821(CGMCC NO.16482 ) and Fusarium verticillioides 0213 (CGMCC NO.16481 ) which are screened out are used, the astragaloside iv is used as a substrate, and through mixed fermentation of the two strains, the cycloastragenol is prepared. A technique for mixed fermentation with two microorganisms is adopted to degrade the astragaloside iv so as to obtain the cycloastragenol high in purity. The technological flow is simple, the cost is low, and the entire technological process is performed at normal temperature and normal pressure. The required equipment condition is low, the method is suitable for mass production, the conversion rate of the final cycloastragenol is as high as 80% or above, and the purity is as high as 95% or above.

The invention provides a blood pressure lowering rice wine and a preparation method thereof. The blood pressure lowering rice wine is prepared through the following steps: (1), weighting 50 parts of sticky rice, 15 parts of rhizoma gastrodiae, 15 parts of radix scutellariae, 10 parts of the roots of kudzu vine and 10 parts of fruit-spike of common selfheal, cleaning up and then evenly mixing; (2), adding 150 parts of water into the raw materials in the step (1), and soaking for a certain time; (3), feeding a paste obtained in the step (2) into an extrusion-expansion device for extrusion-expansion treatment; (4), carrying out ultrasonic microwave treatment on the paste treated in the step (3); (5), adding 0.025 part of saccharomycetes into the paste treated in the step (4); (6), fermenting the paste obtained in the step (5) at 25 DEG C for 5 days; (7), filtering a wine liquid obtained in the step (6) in a bacteria-free environment, so as to obtain the blood pressure lowering rice wine.

The invention discloses a fermentation production culture medium for aspergillus niger capable of selective synthesis of 1,3-diglyceride. The medium is characterized by comprising the following components, by mass, 1.030% of glucose, 1.785% of soybean cake powder, 0.0441% of potassium chloride, 0.044% of disodium hydrogen phosphate, 0.044% of calcium chloride, 0.044% of dipotassium hydrogen phosphate and 0.044% of zinc sulfate, with the balance being water. The medium provided by the invention enables lipase to have greatly improved capability in catalysis of a glycerolysis reaction of glycerin and triglyceride and allows maximum yield of 1,3-diglyceride to reach 26.887%, increased by 2.7 times compared with yield before optimization. The proportion of 1,3-diglyceride in diglyceride is increased by 88.253%, increased by 1.26 times compared with the proportion before optimization.

The invention provides a method for preparing a biological pesticide by utilizing tomato stems and leaves, and is characterized in that the method comprises the following steps: raw material preparation, fermentation, and post-treatment of fermentation liquid. The method has the benefits that the tomato planting wastes, namely tobacco stems and leaves, are used as raw materials, and a stock solution of the fermentation liquid is directly sprayed onto plants for insect killing, bacteria inhibition and plant protection without the need to dry, pulverize, extract and concentrate the materials; atthe same time, the extraction of tomatidine by fermentation is more efficient than the extraction of tomatidine by directly using water, alcohol and acid water; the acidic condition of the fermentation liquid enables tomatin to be converted into the tomatidine, which is less in toxicity; sugar does not need to be removed after the fermentation is finished, and can be used as a plant nutrient andadhesive to facilitate reagent attachment and prevent evaporation of water; in addition, the fermentation reaction conditions are mild, both manpower and material resources are saved, no additional alcohol and acid are needed to be added, the cost is reduced, and no pollution is caused to the environment.

The intention discloses a biological organic fertilizer with a saline-alkali soil improvement effect and a preparation process thereof, and belongs to the technical field of biological fertilizers. According to the present invention, the comprehensive utilization of modern antibacterial active substances and the mixed microbial fermentation technology are integrated, livestock manure, straw, Chinese herb raw materials and seaweed oligosaccharide are fermented through the synergistic effect of microorganisms, the synergistic effect among the raw materials is completely provided, and the efficient and green fermentation biological organic fertilizer is developed. The product has remarkable saline-alkali soil improvement and saline-alkali soil grain yield increase effects, and can promote release of organic matters in soil, improve the physicochemical structure of salinized soil, increase soil aggregate and permeability, improve soil fertility and improve the grain yield increase capacityof saline-alkali soil.

The invention belongs to the technical field of tobacco processing and relates to a method for preparing basic material of a tobacco thin sheet, namely tobacco stem cellulose through the microbial solid fermentation process. Microbes including high-yield prolease, pectolase, ligninase, amylase and other enzymes are selected, and tobacco stems, tobacco smalls and crumbled tobacco are taken as raw materials for preparing microbial seeds, the prepared microbial seeds are inoculated into the tobacco stems for performing solid fermentation, soaking the tobacco stems after fermentation in buffer solution, further performing enzymolysis for degrading the tobacco stems after the enzymolysis, proteins, lignin, pectic substances and other macromolecular substances into micromolecular substances which are easy to be separated from cellulose type substances in the tobacco stems, and further stirring, pulping, filtering and separating for getting water-soluble extract and water-insoluble celluloseof the tobacco stems. The method has the advantages of being simple in process, saving energy, reducing equipment investment and improving the synthetic quality of the tobacco thin sheet, and can greatly reduce the residual quantity of the proteins, pectin, starch and other macromolecules in the tobacco thin sheet.

The invention provides a method for obtaining chlorogenic acid by fermenting leaves of Acer truncatum. The method uses fresh leaves of Acer truncatum as raw materials and utilizes microorganisms suchas Bacillus to ferment the extract of leaves of Acer truncatum, and fully utilizes chlorogenic acid precursor substance in leaves of Acer truncatum to carry out biological transformation in the fermentation process, thereby accumulating chlorogenic acid in large quantities. The method comprises the step: extracting Acer truncatum leaves by breaking walls, preparing fermentation culture medium, inoculating the strain into fermentation culture medium for culture, and centrifuging the supernatant after fermentation to obtain chlorogenic acid for separation and purification. The fermentation culture medium is composed of 10-20g of sucrose, 10-20g of ammonium chloride, 0. 01-0.02g of cop sulfate, 200-400g of maple syrup and 1000mL of water. The method has the advantages of low cost, high yield,high purity, good activity, simple operating and being suitable for industrial production.

The invention discloses a method for improving the oxidation resistance of abalone biological products, and belongs to the technical field of marine organisms. An abalone polypeptide biological product with high in-vitro oxidation resistance is developed by taking abalone protein as a raw material and integrating a modern biological separation technology, a biological enzymolysis technology and amixed bacteria microbial fermentation technology, and mixed bacteria aerobic fermentation strains mainly comprise candida utilis and bacillus subtilis. The obtained abalone polypeptide has strong oxidation resistance capacity, the extracting solution can be subjected to concentration, adsorption drying, alcohol precipitation or resin purification and other treatment according to application ways,and efficient utilization in the fields of health care, foods, medicine and the like is met. The process technology is simple and efficient, and the obtained product is high in oxidation resistance and free radical scavenging capacity, high in stability, low in production cost and easy for industrial large-scale production.

The invention discloses a bio-organic fertilizer capable of efficiently preventing and controlling rice diseases and a preparation method of the bio-organic fertilizer, and belongs to the technical field of the bio-fertilizers. The bio-organic fertilizer integrates the technologies of comprehensive utilization of modern bacteriostatic activity substances and microbial fermentation of mixed bacteria, excrements of livestocks, straw, Chinese herb materials and carrageenan oligosaccharide are fermented through a synergistic effect of microorganisms, the synergistic interaction of materials is given into full play, and the efficient and green fermented bio-organic fertilizer is researched and developed. The bio-organic fertilizer is capable of efficiently inhibiting diseases such as false smutof rice, banded sclerotial blight, rice blast, bacterial leaf blight and the like in the planting of the rice, changing the defect that a chemical bactericide is mainly used in the prevention of therice diseases in the prior art and reducing the drug residue in the planting process of the rice, has an obvious effect of increasing the yield, is simple in production process, relatively low in production cost, and can obviously improve the food safety and the planting benefit of the rice.

The invention provides an oil sludge cleaning and recycling method based on kitchen waste oil. The method is characterized by comprising the following steps: by taking the kitchen waste oil as a raw material, carrying out high-temperature sterilization and then taking the sterilized kitchen waste oil as a carbon source, preparing a nutrient solution, and inoculating a proper amount of strains intothe nutrient solution of a fermentation tank to conduct fermentation for 3-5 days; and by taking the fermentation liquor as washing liquor and controlling the parameters (liquid-solid ratio, temperature, rotating speed, pH and time) of cleaning operation, cleaning oil-containing components in the oil sludge into the washing liquor, and finally separating and recycling oil in the washing liquor through high-speed centrifugal demulsification. On one hand, the harm of kitchen waste oil and oily sludge to the environment can be reduced, and on the other hand, the recycling of oil can be realized.The process is simple, the operation condition is mild, waste is treated with waste in the whole process, waste is turned into wealth, cost is saved, and environment friendliness is achieved.

The invention relates to comprehensive utilization of an aquatic seasoning and an aquatic waste, in particular to a method for fast brewing shrimp oil by taking shrimp heads and shrimp shells as raw materials, belonging to the field of aquaculture. The method for brewing the shrimp oil by taking the shrimp heads or / and the shrimp shells as the raw materials and adding salt comprises the following steps of: (1) crushing the shrimp heads or / and the shrimp shells, and then adding the salt and proteases to form fermentation materials; (2) fermenting for some time by keeping temperature not higher than 60 DEG C, termly stirring in the fermentation process, and controlling a pH value between 7.0 and 8.2; and (3) after fermentation is finished, separating fermentation liquor from fermentation residuals to obtain the fermentation liquor. The invention reduces the production cost by substituting the shrimp heads and the shrimp shells for shrimps; the brewed shrimp oil has strong and comfortable odour without stinking smell, is suitable for being used as a food seasoning, has various nutrient components and is beneficial to the regulation of the balanced intake of nutrient elements and the human health.

The invention discloses undaria pinnatifida / thermal spring water fermentation liquor, a product containing the undaria pinnatifida / thermal spring water fermentation liquor as well as a preparation method and application thereof. The preparation method of the undaria pinnatifida / thermal spring water fermentation liquor comprises the following steps: inoculating lactobacillus into a fermentation substrate, carrying out fermentation culture for 8-20 hours, and sterilizing, so as to obtain the undaria pinnatifida / thermal spring water fermentation liquor, wherein the fermentation substrate comprises undaria pinnatifida powder and thermal spring water. The undaria pinnatifida and the thermal spring water are used as fermentation substrates to prepare the undaria pinnatifida / thermal spring water fermentation liquor, in the preparation process, resource waste is reduced, all plant ingredients are extracted, large utilization of resources is achieved, loss of active ingredients in the undaria pinnatifida is reduced, fermentation conditions are mild, no chemical reagent is adopted, the production cost is reduced, and the undaria pinnatifida / thermal spring water fermentation liquor is suitable for industrial production. Environmental pollution is reduced, energy conservation and environmental protection are achieved, and ideal anti-oxidation and moisturizing effects are achieved.

The invention discloses a fermentation production culture medium for aspergillus niger capable of selective synthesis of 1,3-diglyceride. The medium is characterized by comprising the following components, by mass, 1.030% of glucose, 1.785% of soybean cake powder, 0.0441% of potassium chloride, 0.044% of disodium hydrogen phosphate, 0.044% of calcium chloride, 0.044% of dipotassium hydrogen phosphate and 0.044% of zinc sulfate, with the balance being water. The medium provided by the invention enables lipase to have greatly improved capability in catalysis of a glycerolysis reaction of glycerin and triglyceride and allows maximum yield of 1,3-diglyceride to reach 26.887%, increased by 2.7 times compared with yield before optimization. The proportion of 1,3-diglyceride in diglyceride is increased by 88.253%, increased by 1.26 times compared with the proportion before optimization.

In the present invention, a Tremella fuciformis strain (Tremella fuciformis) was screened out, the strain name is XY, and it was deposited in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation date is April 25, 2019, and the preservation number is CGMCC No.17675. The present invention also provides the application of the above Tremella fungus strains in the production of Tremella polysaccharides, the main process of which is to insert the seed liquid prepared from CGMCC No. 17675 into a solid-state fermentation medium for fermentation to obtain a fermentation liquid. It can prepare high-molecular-weight polysaccharides with a molecular weight of 1000-1500kDa and low-molecular-weight polysaccharides with a molecular weight of 100-300kDa. The total output can reach 15-25g / L, and the protein content is less than 0.3%. Compared with the liquid fermentation method, it has a significant cost advantage. Tremella It has a good application prospect in the industrial production of polysaccharides.

The invention discloses a single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid, which comprises the procedures including that: firstly, seed liquid of high-activity actinobacillus capable of producing succinic acid is prepared; secondly, the seeds are proliferated in cultivation with a10L pot; thirdly, the 10L-pot solified cells are prepared; fourthly, the repeated single-pot fermentation with the 10L pot is carried out, then the seeds are inoculated into a fresh substrate, the first batch of fermentation is controlled within 24 hours, followed by 9 batches of continuous fermentation, each of which lasts for 16 hours, then the amphoteric anaerobic cultivation pattern is controlled through the current velocity of the combined air of CO2 and H2, the pH of the fermentation liquid is controlled with fed-batch of calcium oxide, wherein, at the end of each batch of fermentation, the next batch of fermentation is carried out through intercepting the solidified cells with positive-pressure sterilized operation with a propeller filter arranged inside the pot, the fermentation intensity is stabilized above 3.10g / (h.L), and the single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid is completely accomplished.