53results about How to "Many peaks" patented technology

The invention provides a method for identifying HPLC fingerprints for protecting ginseng of origin, which is characterized by comprising the following steps: A, establishing liquid phase chromatograph fingerprints of the ginseng of origin, namely using the ginseng in the same habitat as a standard, analyzing the ginseng by liquid phase chromatograph and establishing fingerprints; and B, detectinga sample, namely taking a ginseng sample to be detected, detecting the sample under the same conditions as the standard respectively to obtain a spectrogram of the detected sample, and analyzing the fingerprints of the detected sample and the standard by a direct observational method or fingerprints software as qualitative basis. The method has simple pretreatment for fingerprints of the ginseng medicinal material established by adopting the liquid phase chromatograph, multiple peak numbers and better separation degree, therefore, contents of main chemical compositions of the ginseng medicinal material have large difference caused by different growing geographic environments and climates. The HPLC fingerprints of the ginseng can provide reference for distinguishing high-end ginseng health-care products from origin places and non-origin places, and provide reference for establishing fingerprints of other traditional Chinese medicinal materials. The research of methodology shows that all the precision, the stability and the repeatability have better application prospect.

The invention discloses a canzhiling oral solution fingerprint map building method. The map includes the following steps of preparation of a test solution, preparation of a reference solution, testing through a high performance liquid chromatograph and processing of data and a map. The invention further discloses a Canzhiling oral solution fingerprint map and a method for utilizing the fingerprint map to control quality of a Canzhiling oral solution. The Canzhiling oral solution fingerprint map building method is simple in operation, stable, reliable, high in accuracy and high in separation degree, the fingerprint map is high in stability and reproducibility and large in information quantity, and the fingerprint map is adopted as a quality control means for the Canzhiling oral solution, so that one-sidedness caused by judging of overall quality of a preparation by testing one or two chemical ingredients is avoided, and probability of artificial processing in order to enabling quality to be up to standards is lowered; samples of multiple batches are analyzed systematically, so that quality of the Canzhiling oral solution can be evaluated more comprehensively and scientifically, and product quality and efficacy are guaranteed.

The invention discloses a raw fleece-flower root HPLC fingerprint detection method. The detection method comprises the following steps: (1) preparing a control substance solution; (2) preparing a reference substance solution; (3) establishing a control fingerprint; (4) preparing a test article solution; (5) detecting the test article solution. The obtained fingerprint has the advantages that there are many peaks, the resolution and repeatability are good, the precision is high, and the quality of raw fleece-flower root can be effectively monitored. By using the extraction method and chromatogram conditions of provided fingerprint detection method, contents of 16 components of raw fleece-flower root can be measured, and thus the quality of raw fleece-flower root can be monitored from more aspects.

The present invention relates to a kind of fingerprint detection method of Wenjing Decoction, described method, the steps are as follows: 1) preparation of test solution: take by weighing 1-2g each of Angelica sinensis, Rhizoma Chuanxiong, Radix Paeoniae Alba, Cinnamon, Cortex Moutan, and Curcuma officinalis powder, Ginseng, licorice, and Achyranthes bidentata powder are 3-4g each, add 250-350mL of water, mix well, first heat to boiling with strong fire, then switch to slow fire and fry until the decoction reaches about 150-170mL, filter while hot, and the filtrate Add water to make up to 240‑260mL; accurately draw 8‑12mL of Wenjingtang standard decoction, add methanol to make up to 40‑60mL, airtight, weigh, ultrasonicate for 8‑12min, leave overnight, weigh again, use methanol Make up the lost quality, shake well, filter, and get the filtrate to get final product; 2) detection: take 5-15 μL of the test solution obtained in the previous step, inject it into a high-performance liquid chromatograph, and obtain a chromatogram; 3) result Judgment: The chromatogram obtained in the previous step is compared with the standard control fingerprint. If the similarity is greater than 90%, the sample is qualified.