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Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof

A technology of fingerprints and determination methods, which is applied in the field of traditional Chinese medicine fingerprint analysis, can solve problems such as poor resolution of fingerprints, and achieve the effects of good absorption value, high similarity, and easy identification

Active Publication Date: 2014-06-18
贵州汉方制药有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In the patent document with the announcement number "CN101327246B" and the title "Fingerprint Detection Method and Standard Fingerprint of Astragalus medicinal materials, intermediates and their preparations", 80%-95% methanol is used as the extraction solvent, and the acetonitrile-water system is used as the extraction solvent. Mobile phase system, the resolution of the fingerprint spectrum obtained is not very good, only 19 characteristic chromatographic peaks

Method used

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  • Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof
  • Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof
  • Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof

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Effect test

Embodiment 1

[0047] Embodiment 1 of the present invention: the assay method of Radix Astragali granule fingerprint comprises the following steps:

[0048] (1) Preparation of the test solution: crush Astragalus granules, pass through a 60-mesh sieve, accurately weigh 1.0g of the powder, add 10mL of 10% methanol solution to dissolve, ultrasonically extract for 5min, then cool, make up for the lost weight, shake well, filter After passing, take the continued filtrate and pass through a 0.45 μm microporous membrane to obtain the test solution;

[0049] (2) Preparation of the reference substance solution: take the reference substance of acteocoside, formononetin, acteosin, and formononetin, which were dried under reduced pressure to constant weight, and add methanol respectively to make each 1mL containing 0.562mg of acteosin Glycoside, 0.254mg formononetin, 0.357mg calycosin, 0.274mg formononetin reference substance solution (stored in a refrigerator at 4°C for later use);

[0050] (3) Determ...

Embodiment 2

[0052] Embodiment 2 of the present invention: the assay method of Radix Astragali granule fingerprint comprises the following steps:

[0053] (1) Preparation of the test solution: crush Astragalus granules, pass through a 60-mesh sieve, accurately weigh 1.0g of the powder, add 10mL of 10% methanol solution to dissolve, ultrasonically extract for 10min, then cool, make up for the lost weight, shake well, filter After passing, take the continued filtrate and pass through a 0.45 μm microporous membrane to obtain the test solution;

[0054] (2) Preparation of the reference substance solution: take the reference substance of acteocoside, formononetin, acteosin, and formononetin, which were dried under reduced pressure to constant weight, and add methanol respectively to make each 1mL containing 0.562mg of acteosin Glycoside, 0.254mg formononetin, 0.357mg calycosin, 0.274mg formononetin reference substance solution (stored in a refrigerator at 4°C for later use);

[0055] (3) Deter...

Embodiment 3

[0057] Embodiment 3 of the present invention: the assay method of Radix Astragali granule fingerprint comprises the following steps:

[0058] (1) Preparation of the test solution: crush Astragalus granules, pass through a 60-mesh sieve, accurately weigh 1.0g of the powder, add 10mL of 10% methanol solution to dissolve, ultrasonically extract twice, each time for 30min, then cool to make up for the lost weight , shake well, filter, take the continued filtrate and pass through a 0.45 μm microporous membrane to obtain the test solution;

[0059] (2) Preparation of the reference substance solution: take the reference substance of acteocoside, formononetin, acteosin, and formononetin, which were dried under reduced pressure to constant weight, and add methanol respectively to make each 1mL containing 0.562mg of acteosin Glycoside, 0.254mg formononetin, 0.357mg calycosin, 0.274mg formononetin reference substance solution;

[0060] (3) Determination: Chromatographic conditions: Agil...

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Abstract

The invention discloses a measurement method of a radix astragali granule fingerprint. The measurement method of the radix astragali granule fingerprint comprises the steps of preparation of a test solution, preparation of a comparison product solution, high performance liquid chromatograph measurement, and treatment of data and fingerprint. The invention further discloses the radix astragali granule fingerprint obtained by the method. Compared with the prior art, the radix astragali granule fingerprint obtained by the invention has the characteristics of more peaks, good peak form, good peak separation effect, larger peak area, easiness of verification, high similarity, accuracy and reliability. The method provided by the invention is simple and convenient, fast, accurate, stable and reliable, can be used for the quality control of a radix astragali granule sample, can be used for comprehensively, objectively and scientifically evaluating the quality of radix astragali granules, and provides the foundation and the reference for the rational drug use and further drug efficacy study of the radix astragali granules.

Description

technical field [0001] The invention relates to a method for analyzing fingerprints of traditional Chinese medicines, in particular to a method for measuring HPLC characteristic fingerprints of Astragalus granules and the characteristic fingerprints of Astragalus granules obtained by the method. Background technique [0002] Most of the traditional Chinese medicines and their preparations in my country lack strict quality standards and scientific testing methods, so it is difficult to effectively control their internal quality, cannot guarantee the safety and effectiveness of medication, and do not meet the requirements of the international pharmaceutical market, which seriously restricts the Chinese medicine industry in my country. development of. Strengthening the research on the quality control of traditional Chinese medicinal materials is a key issue in the standardization and standardization of traditional Chinese medicine. Only by carrying out scientific quality contro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 周欣陈华国赵超龚小见赵杨邓杰
Owner 贵州汉方制药有限公司
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