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Method for establishing fingerprints of Dan'e Fukang decoction and its standard fingerprints and applications

A technology of Dan'e Fukang decoction and Efukang decoction, which is applied in the field of traditional Chinese medicine fingerprint analysis, can solve the problem of inability to reflect the integrity characteristics of traditional Chinese medicine compound preparations, the lack of detection methods for Dan'e Fukang decoction, and incomplete drug quality and other issues, to achieve the effect of reducing the possibility of human processing, improving quality control technology, and good absorption value

Active Publication Date: 2020-01-03
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, Dan'e Fukang decoction is a compound preparation with complex ingredients. It is obviously not comprehensive to control the quality of the drug simply by detecting the quantity of one or two index components, nor can it reflect the overall characteristics of traditional Chinese medicine compound preparations.
However, because Dan'e Fukang decoction contains many medicinal materials and complex components, in the prior art, there is no method for detecting Dan'e Fukang decoction

Method used

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  • Method for establishing fingerprints of Dan'e Fukang decoction and its standard fingerprints and applications
  • Method for establishing fingerprints of Dan'e Fukang decoction and its standard fingerprints and applications
  • Method for establishing fingerprints of Dan'e Fukang decoction and its standard fingerprints and applications

Examples

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Effect test

specific Embodiment approach

[0094] The present invention is described in detail below with reference to Examples and drawings. Those skilled in the art can understand that the following examples are for the purpose of illustration and should not be construed as limiting the present invention in any way. The protection scope of the present invention is defined by the appended claims.

[0095] Methods of Quality Control of Dan'e Fukang Decoction

[0096] The method for quality control of Dan'e Fukang decoction comprises the following steps:

[0097] (1) Construction of the fingerprint of Dan'e Fukang decoction to be tested: take the Dan'e Fukang decoction to be tested, and construct the fingerprint of the Dan'e Fukang decoction to be tested according to the method described in this application;

[0098] (2) Import the fingerprint of Dan'e Fukang decoction to be tested into "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" 2004A version and compare the similarity with the contr...

Embodiment 1

[0100] Example 1: Optimizing the conditions of the method for detecting Dan'e Fukang decoction

[0101] The construction method of the fingerprint of the present invention is obtained through scientific experiments and screening, and is not a conventional choice in the field. The present invention mainly optimizes the preparation method of the test solution, chromatographic conditions, detection wavelength and analysis time. details as follows.

[0102] (1) Selection of the preparation method of the test solution: figure 1 The test compared the influence of different extraction solvents on the extraction effect of the sample solution, such as 100% MeOH, 100% EtOH, 50% MeOH, 70% EtOH, 50% EtOH ( figure 1 From bottom to top are 100% MeOH, 100% EtOH, 50% MeOH, 70% EtOH, 50% EtOH); figure 2 The test compared the influence of different extraction methods on the extraction effect of the sample solution: reflux extraction for 30min, 60min, 120min, ultrasonic extraction for 15min...

Embodiment 2

[0109] Example 2: Detection of Dan'e Fukang Decoction Using Chromatography

[0110] In this example, chromatographic method is used to detect Dan'e Fukang decoction. In this embodiment, the chromatographic method is high performance liquid chromatography, and the Dan'e Fukang decoction is a nourishing agent of Dan'e Fukang decoction. The conditions used in this example are the experimental conditions obtained according to the above optimization.

[0111] The concrete steps of this embodiment are:

[0112] (1) Preparation of the test solution: Take 5g of Dan'e Fukang decoction, add 25mL of 70% ethanol solution accurately, after precise weighing, shake well, ultrasonically extract for 15min, and make up the lost weight with 70% ethanol after cooling , filtered through a microporous membrane, and the filtrate was taken.

[0113] (2) Preparation of reference substance solution:

[0114] Take an appropriate amount of gallic acid reference substance, add methanol to make a sol...

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Abstract

The invention relates to a Dan'e Fukang soft extract fingerprint establishment method, and a Dan'e Fukang soft extract standard fingerprint and applications thereof. In the prior art, the Dan'e Fukang soft extract contains ten traditional Chinese medicines, wherein the components are complex and diverse, and the method for detecting the Dan'e Fukang soft extract does not exist. The invention provides a method for detecting the Dan'e Fukang soft extract by preparing a Dan'e Fukang soft extract sample solution and a control substance solution through a chromatography method, a method for establishing the Dan'e Fukang soft extract standard fingerprint by detecting multiple batches of Dan'e Fukang soft extract samples, and a method for controlling the quality of the Dan'e Fukang soft extract by analyzing the similarity of the fingerprints of various Dan'e Fukang soft extract samples.

Description

technical field [0001] The invention relates to a method for analyzing fingerprints of traditional Chinese medicines, in particular to a method for establishing HPLC fingerprints of Dan'e Fukang decoction and the fingerprint of Dan'e Fukang decoction obtained by the method. Background technique [0002] Dan'e Fukang Decoction is a traditional Chinese medicine compound preparation made from ten medicinal materials of purple salvia miltiorrhiza, curcuma, bamboo leaf bupleurum, notoginseng, red peony root, angelica, sanleng, Cyperus cyperi, Corydalis corydalis, and licorice. The ointment in traditional Chinese medicine preparations has the functions of promoting blood circulation and removing blood stasis, soothing the liver and regulating qi, regulating menstruation and relieving pain, softening hardness and resolving accumulation, etc. It is a commonly used drug for clinical treatment of endometriosis (Endometriosis, EMs). [0003] Dan'e Fukang decoction is in a dark brown vi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 黄春跃刘欣胡晓牛莉鑫范思洋许慧张伟
Owner SHANGHAI INST OF PHARMA IND CO LTD
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