37results about How to "Long excitation wavelength" patented technology

The invention provides an organic luminous material and a preparation method thereof, and relates to an organic silicon polymer. Raw materials of the organic luminous material comprise metallic organic substance and polycarbosilane. The preparation method comprises the following steps of: putting polydimethylsiloxane into a reaction kettle, heating the polydimethylsiloxane under the protection ofargon, inflating the argon to the reaction kettle, adding n-hexane into the reaction kettle to dissolve the product after the temperature in the reaction kettle is naturally cooled to room temperature, filtering the product, removing the n-hexane in vacuum to obtain the polycarbosilane, soaking the ground polycarbosilane into ethanol, flushing the insoluble substances by using the ethanol and then drying the insoluble substances in vacuum; and pouring the polycarbosilane powder and the metallic organic substance powder into a three-necked bottle, heating the mixed powder to between 150 and 160 DEG C under the protective atmosphere of argon, preserving the heat for 1 to 1.5 hours, heating the mixed powder to between 260 and 270 DEG C, preserving the heat for 1 to 1.5 hours, dissolving the product by using the dry n-hexane after the reaction is finished and the product is cooled, filtering the product to remove the insoluble substances, and finally distilling the product with reduced pressure to obtain the powdery solid organic luminous material.

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate and further comprises a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad which are lapped and pasted on the bottom plate in sequence. A carbendazim monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A carbendazim hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the qualitycontrol area. A carbendazim hapten is obtained in the mode of reacting of 2-aminobenzimidazole with butanedioic anhydride. The invention further provides the preparation method of the test strip and amethod for detecting the carbendazim in samples by applying the test strip. The time resolution fluorescent immunochromatographic test strip and the detecting method provided by the invention have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the carbendazim in the large-batch samples can be detected quickly and monitored on site.

Provided are preparation and application of a mercury ion fluorescent probe compound based on rhodamine B. The invention relates to the mercury ion fluorescent probe compound and the preparation and the application thereof, wherein the mercury ion fluorescent probe compound has the structure represented by the formula I. The preparation method comprises the steps: carrying out a reaction of 2,2'-bipyridine with methyl lithium under the protection of nitrogen gas to obtain 6-methyl-2,2'-bipyridine, then oxidizing methyl by selenium dioxide to obtain 6-aldehyde-2,2'-bipyridine; and carrying out a reaction of 6-aldehyde-2,2'-bipyridine and rhodamine B hydrazide to obtain the final product probe molecule. The probe molecule has good selectivity and sensitivity to mercury ions, has no toxicity to cells, and not only can be used for detection of mercury ions in water bodies but also can be used for detection and imaging of mercury ions in cells.

The invention discloses near-infrared fluorescent powder. A chemical general formula of the near-infrared fluorescent powder is Mg(3-x)Ga2CrxGeO8, wherein x is greater than 0 and is smaller than or equal to 0.1. The invention also discloses a preparation method of the near-infrared fluorescent powder. The preparation method comprises the following steps: weighing oxide or carbonate containing elements of Mg, Ga, Cr and Ge according to molar proportions of all elements in the chemical general formula Mg(3-x)Ga2CrxGeO8 of the near-infrared fluorescent powder, mixing, and grinding, thus obtaininga mixture; heating the mixture to 1200 to 1300 DEG C, and roasting for 3 to 4 hours, thus obtaining a sintered body; cooling the sintered body to room temperature, and fully grinding, thus obtainingthe near-infrared fluorescent powder. Meanwhile, the invention discloses application of the near-infrared fluorescent powder. The near-infrared fluorescent powder provided by the invention has longerexcitation wavelength and cannot be easily absorbed by a living body, an emission spectrum is in wide spectrum emission, the wavelength covers 700 to 1200nm which can cover one part of a first windowand a second window of a body biological window, the luminous strength is high, the stability is high, the preparation technology of the near-infrared fluorescent powder is simple, operation and control are easy, the safety is high, the preparation time is short, and the near-infrared fluorescent powder is convenient for large-scale production as well as popularization and application.

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting butralin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate and further comprises a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad which are lapped and pasted on the bottom plate in sequence. A butralin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A butralin antigen-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality controlarea. A butralin antigen is obtained in the mode of reacting of 4-chlorine-3,5-binitro phenylacetic acid with butyl amine. The invention further provides the preparation method of the test strip and amethod for detecting the butralin in samples through the test strip. The time resolution fluorescent immunochromatographic test strip and the detecting method provided by the invention have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the butralin in the large-batch samples can be detected quaickly and monitored on site.

The invention features a fluorescent spectroscopic method for determining molecular affinities of test compounds for human serum albumin.

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate, a sample absorbent pad, a combination absorbent pad, a nitrocellulose membrane and a water absorbent pad. A pendimethalin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination absorbent pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A pendimethalin hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality control area. A pendimethalin hapten is obtained in the modes that N-(1-ethyl propyl ether)-3,4-methyl toluidine and 4-bromo-butyric acid ethyl ester react to generate hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, then the hydrocarbylation N-(1-ethyl propylether)-3,4-methyl toluidine reacts with concentrated nitric acid to generate nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, and then the nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with KOH. The time resolution fluorescent immunochromatographic test strip and a detecting method provided by the invention have the advantages that operationis easy, sensitivity is high, the detecting speed is high, and the cost is low.

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting flumetralin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate, a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad. A flumetralin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A flumetralin hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality control area. A flumetralin hapten is obtained in the modes that 2-chlorine-1,3-binitro-5-benzotrifluoride reacts with 3-amidogen-3-(2-chlorine-6-fluorophenyl)-propionic acid to generate 3-(2-chlorine-6-fluorophenyl)-3-(2,6-binitro-4-trifluoromethyl-anilino)-propionic acid, and then the 3-(2-chlorine-6-fluorophenyl)-3-(2,6-binitro-4-trifluoromethyl-anilino)-propionic acid reacts with iodoethane. The time resolution fluorescent immunochromatographic test strip and a detecting method have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the flumetralin in large-batch samples can be detected quickly.

The invention discloses a compound I, a preparation method thereof and application of the compound I as a fluorescent probe. The compound I has near-infrared excitation / emission, the excitation wavelength ranges from 500 nm to 750 nm; and the compound is excited at a maximum absorption wavelength of 670 nm, and the emission wavelength is 700 to 810 nm. In addition, the compound I has an ultra-highsensitivity response to the PGP-1 enzyme. The detection limit for the PGP-1 enzyme at lambda ex / em=670 / 700 nm is 0.18 ng / mL. The compound I is used as a fluorescent probe to realize PGP-1 in vivo detection and fluorescence imaging for the first time. In vitro, cell and in vivo PGP-1 detection and fluorescence imaging can be realized, and all cells and tissues with inflammatory response and canceration can be assayed and imaged.

The invention discloses a time-resolved fluorescence immunochromatography test strip for detecting acetamiprid, and a preparation method and an application thereof. The test strip comprises a bottom plate, and a sample absorption pad, a conjugate release pad, a nitrocellulose membrane and a water absorption, which are sequentially lapped and adhered on the bottom plate, wherein an acetamiprid monoclonal antibody labelled by a fluorescent microsphere is buried on the conjugate release pad, a detection area and a quality control area are fixed on the nitrocellulose membrane, the detection area is coated with acetamiprid hapten-carrier protein conjugate, the quality control area is coated with a goat anti-mouse anti-antibody, and the acetamiprid hapten is obtained by a reaction of N-noracetamidine and an aminobutyric acid. The invention further provides a preparation method of the test strip and a method for detecting the acetamiprid in a sample by using the above test strip. The test strip and the detection method provided by the invention have the advantages of simple operation, high sensitivity, high detection speed and low cost, and can be used for realizing rapid detection and onsite monitoring of the acetamiprid in mass samples.

The invention provides an organic luminous material and a preparation method thereof, and relates to an organic silicon polymer. Raw materials of the organic luminous material comprise metallic organic substance and polycarbosilane. The preparation method comprises the following steps of: putting polydimethylsiloxane into a reaction kettle, heating the polydimethylsiloxane under the protection ofargon, inflating the argon to the reaction kettle, adding n-hexane into the reaction kettle to dissolve the product after the temperature in the reaction kettle is naturally cooled to room temperature, filtering the product, removing the n-hexane in vacuum to obtain the polycarbosilane, soaking the ground polycarbosilane into ethanol, flushing the insoluble substances by using the ethanol and then drying the insoluble substances in vacuum; and pouring the polycarbosilane powder and the metallic organic substance powder into a three-necked bottle, heating the mixed powder to between 150 and 160 DEG C under the protective atmosphere of argon, preserving the heat for 1 to 1.5 hours, heating the mixed powder to between 260 and 270 DEG C, preserving the heat for 1 to 1.5 hours, dissolving the product by using the dry n-hexane after the reaction is finished and the product is cooled, filtering the product to remove the insoluble substances, and finally distilling the product with reduced pressure to obtain the powdery solid organic luminous material.

The invention belongs to the technical field of medical materials, and discloses a method for activating an anti-tumor compound through biological orthogonal photocatalytic oxidative dehydrogenation, a composition and application. The method comprises the following step: carrying out dehydrogenation reaction on dihydrobenzophenanthridine alkaloid under the conditions of illumination and oxygen and under the action of a photocatalyst to obtain the benzophenanthridine alkaloid. The composition is prepared from the dihydrobenzophenanthridine alkaloid and a photocatalyst. The invention also discloses application of the composition in preparation of a cell nucleus targeted light-activated imaging agent and / or a light-activated anti-tumor product. The oxidative dehydrogenation method is simple and efficient; the dihydrobenzophenanthridine alkaloid and the photocatalyst are high in light activation efficiency under the aerobic condition; the fluorescent probe is used in cells, is illuminated under the oxygen condition, can realize nucleus-targeted specific light-activated fluorescence imaging in living cells, and has the advantages of high light activation efficiency, low cytotoxicity and the like. Through illumination control, the composition disclosed by the invention has a relatively good anti-tumor effect.

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting metalaxyl, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate and further comprises a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad which are lapped andpasted on the bottom plate in sequence. A metalaxyl monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality controlarea are fixed to the nitrocellulose membrane. A metalaxyl hapten-carrier protein conjugate is sprayed in the detecting area, an anti-sheep and anti-mouse antibody is sprayed in the quality control area, and a metalaxyl hapten is obtained in the modes that N-(2,6-dimethyl phenyl) alanine methyl ester reacts with (4-nitrophenoxy) acetylchloride to generate nitrobenzene metalaxyl, and then the nitrobenzene metalaxyl is subjected to zinc powder reduction. The time resolution fluorescent immunochromatographic test strip and a detecting method provided by the invention have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the metalaxyl in large-batch samples can be detected quickly and monitored on site.

The invention discloses near-infrared fluorescent powder. A chemical general formula of the near-infrared fluorescent powder is Mg(3-x)Ga2CrxGeO8, wherein x is greater than 0 and is smaller than or equal to 0.1. The invention also discloses a preparation method of the near-infrared fluorescent powder. The preparation method comprises the following steps: weighing oxide or carbonate containing elements of Mg, Ga, Cr and Ge according to molar proportions of all elements in the chemical general formula Mg(3-x)Ga2CrxGeO8 of the near-infrared fluorescent powder, mixing, and grinding, thus obtaininga mixture; heating the mixture to 1200 to 1300 DEG C, and roasting for 3 to 4 hours, thus obtaining a sintered body; cooling the sintered body to room temperature, and fully grinding, thus obtainingthe near-infrared fluorescent powder. Meanwhile, the invention discloses application of the near-infrared fluorescent powder. The near-infrared fluorescent powder provided by the invention has longerexcitation wavelength and cannot be easily absorbed by a living body, an emission spectrum is in wide spectrum emission, the wavelength covers 700 to 1200nm which can cover one part of a first windowand a second window of a body biological window, the luminous strength is high, the stability is high, the preparation technology of the near-infrared fluorescent powder is simple, operation and control are easy, the safety is high, the preparation time is short, and the near-infrared fluorescent powder is convenient for large-scale production as well as popularization and application.

The invention discloses glycosyl beta-elemene derivatives having general formulae I, II and III. Researches indicate that the glycosyl beta-elemene derivatives have good biological water solubility and good anti-cancer activities, can be applied to malignant tumor treatment as an anti-cancer medicine for realizing real-time tumor monitoring and treatment, and have important scientific meaning to researches and application of beta-elemene in the field of medicine. Furthermore, the invention provides a method for preparing the corresponding compounds. The method applies a click chemical reaction technology, and is simple and scientific in synthesis.

The invention provides a composite resin aerogel, which is obtained by compounding an organic resin prepared from glycidyl methacrylate, divinyl benzene and ethylene glycol dimethacrylate with activated cellulose, then modifying with 3-alpha-furyl acrylic acid, and carrying out circulating freeze drying; or compounding the activated cellulose with organic resin, modifying with 3-alpha-furyl acrylic acid, loading a photocatalyst, and carrying out circulating freeze drying to obtain the product. The invention further provides a specific preparation method of the composite resin aerogel and an application method of the composite resin aerogel in sewage treatment, the process is simple, the effect is excellent, the aerogel is wrapped in the stainless steel grids, so that the contact area between the aerogel and sewage is large enough, the integrity of the aerogel is protected, and the aerogel can be repeatedly used; the composite resin aerogel is high in porosity, large in specific surface area, excellent in shape recovery performance, excellent in heavy metal ion adsorption performance, capable of being resolved and regenerated, capable of being recycled for multiple times and good in application potential.

A time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin, a preparation method and application thereof. The test strip includes a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane, and an absorbent pad. The conjugate release pad is embedded with pendimethalin monoclonal antibody labeled with fluorescent microspheres, and fixed on the nitrocellulose membrane. There are detection area and quality control area. The detection area is sprayed with pendimethalin hapten-carrier protein conjugate, and the quality control area is sprayed with goat anti-mouse anti-antibody. The pendimethalin hapten is composed of N‑(1‑B N-(1-ethylpropyl)-3,4-dimethylaniline reacts with ethyl 4-bromobutyrate to generate alkylated N-(1-ethylpropyl)-3,4-dimethylaniline, which is then reacted with concentrated nitric acid Generate nitroalkylated N‑(1‑ethylpropyl)‑3,4‑dimethylaniline, and then react with KOH to get it. The test strip and detection method provided by the invention have the advantages of simple operation, high sensitivity, fast detection speed and low cost.

The invention provides a carboxylesterase fluorescent probe of a structure of formula I. The carboxylesterase fluorescent probe provided by the invention is a hemicyanine compound with a naphthalene nuclear two-photon absorption unit and a D-pi-A structure, has the two-photon absorption and emission properties, and can be applied to detection on intracellular carboxylesterase. Experiment results of the embodiment show that the carboxylesterase fluorescent probe provided by the invention is capable of prolonging excitation wavelengths (800nm) of carboxylesterase in cells and tissue, reducing optical damage to cells and tissue and increasing tissue penetration depths and imaging depths; meanwhile, when being adopted to detect carboxylesterase, the carboxylesterase fluorescent probe providedby the invention is good in stability, short in response time (7 minutes), no sensitive to pH values, good in specificity for intracellular carboxylesterase imaging, and capable of detecting carboxylesterase in living cells under a two-photon excitation condition.

A kind of ratio-type pH probe with cell organelle or protein targeting function and its application belongs to the field of fine chemical industry. In the field of biological imaging, the visualization of cell activities is of great significance. Ratio pH probes located in organelles mainly achieve organelle targeting by connecting specific Tags, such as introducing morpholine to achieve lysosome positioning, introducing triphenylphosphine to achieve mitochondrial targeting, and using Hoechst to achieve DNA targeting, etc. . The introduction of Tag sites directly on the basis of ratiometric pH probes often has a series of problems such as increased synthesis steps and increased separation difficulty, so it is very important to develop a general platform. BODIPY and rhodamine have the advantages of high molar extinction coefficient and fluorescence quantum yield, narrow spectrum, and spectral matching, which are very suitable for the construction of ratiometric pH probes with FRET mechanism. These compounds have high biocompatibility and can specifically bind to ratiometric pH. , and also has the function of organelle targeting.

The invention belongs to the technical field of biological fluorescence imaging, and provides a near-infrared two-region microscopic imaging system and method based on laser scanning, and the method comprises the steps: carrying out the beam deflection and beam combination of laser beams with different wavelengths, and obtaining combined light; shaping the combined light to obtain shaped light; phase-modulating the shaped light to generate a multi-focus illumination light field; relaying and two-dimensional scanning are carried out on the shaped light subjected to phase modulation, so that a multi-focus illumination light field is subjected to two-dimensional scanning on a focal plane of a microobjective, a sample is excited to emit a fluorescence signal of a near-infrared second-area wave band, and a near-infrared camera is used for detecting the fluorescence signal and imaging. According to the scheme, an optical path system is simple to integrate, and deeper imaging can be realized due to longer excitation wavelength; and the dye selection of the near-infrared second region is more flexible.

The invention discloses a time resolved fluorescence immunochromatography test strip for detecting coumarin as well as a preparation method and application thereof. The test strip comprises a bottom plate, a sample absorption pad, a compound release pad, a nitrocellulose membrane and a water absorption pad, wherein the sample absorption pad, the combined object release pad, the nitrocellulose membrane and the water absorption pad are sequentially lapped and stuck; a fluorescent microsphere marked coumarin monoclonal antibody is embedded on the compound release pad; a detecting region and a quality control region are fixed on the nitrocellulose membrane; a coumarin hapten-carrier protein conjugate is sprayed and coated on the detecting region; an anti-goat anti-mouse antibody is sprayed andcoated on the quality control region; the coumarin hapten is obtained from coumarin through aldolization. The invention also provides the preparation method of the test strip and a method for using the coumarin in a test strip detecting sample. The test strip and the detecting method provided by the invention have the advantages of operation simplicity, high sensitivity, high detecting speed andlow cost; the fast detection and field monitoring on coumarin in a great batch of samples can be realized.

The invention discloses a time-resolved fluorescence immunochromatographic test strip for detecting quinclorac, and a preparation method and an application thereof. The test strip comprises a bottom plate, and a sample absorption pad, a conjugate release pad, a nitrocellulose membrane and a water absorption pad which are sequentially lapped and pasted on the bottom plate; the conjugate release padis buried with a fluorescent microsphere-labeled quinclorac monoclonal antibody; a detection area and a quality control area are fixed on the nitrocellulose membrane; the detection area is coated with a quinclorac hapten-carrier protein conjugate; the quality control area is sprayed with a goat anti-mouse anti-antibody, wherein the quinclorac hapten obtained by the steps of enabling quinclorac tobe reacted with (1, 3-dioxanylethyl) magnesium bromide to generate propylal quinclorac, and then reacting with trifluoroacetic acid. The test strip and the detection method provided by the inventionhave the advantages of simplicity in operation, high sensitivity, high detection speed, low cost and capability of realizing rapid detection and on-site monitoring of quinclorac in a large quantity ofsamples.

A time-resolved fluorescent immunochromatographic test strip for detecting metalaxyl, its preparation method and application. The test strip includes a bottom plate and a sample absorption pad, a conjugate release pad, a nitrocellulose membrane, and a water absorption pad that are successively overlapped and pasted on the bottom plate. The metalaxyl sheet labeled with fluorescent microspheres is embedded on the conjugate release pad. Cloned antibody, the nitrocellulose membrane is fixed with a detection area and a quality control area, the detection area is sprayed with a metalaxyl hapten-carrier protein conjugate, and the quality control area is sprayed with a goat anti-mouse anti-antibody, The metalaxyl hapten is generated by the reaction of N-(2,6-dimethylphenyl) alanine methyl ester and (4-nitrophenoxy) acetyl chloride, and then passed Zinc powder can be obtained by reduction. The test strip and detection method provided by the invention have the advantages of simple operation, high sensitivity, fast detection speed and low cost, and can realize rapid detection and on-site monitoring of metalaxyl in large batches of samples.

The invention discloses a method for on-line fluorescence detection of a water form in a soil cultural relic. The method comprises the following steps: introducing an FE fluorescent probe into a detected soil cultural relic, detecting a fluorescence spectrum of the soil cultural relic marked by the FE fluorescent probe, and identifying free water and bound water in the soil cultural relic according to the position and change of a characteristic peak in the fluorescence detection spectrum. The method has great significance in evaluating the water physical property of the soil cultural relics. According to the method, FE (4-diethylamino-3-hydroxyflavone) is used as a fluorescent probe, FE is a fluorescent probe sensitive to the water form, the fluorescent probe has a long excitation wavelength (400nm), the defect that the fluorescence intensity of a soil sample is reduced due to absorption generally existing in the soil sample during ultraviolet excitation is overcome, and the method has wide adaptability.

A time-resolved fluorescent immunochromatographic test strip for detecting acetamiprid, its preparation method and application. The test strip includes a bottom plate and a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane, and a water absorbent pad that are sequentially overlapped and pasted on the bottom plate, and the conjugate release pad is embedded with acetamiprid monoclonal antibody labeled with fluorescent microspheres. , the detection area and the quality control area are fixed on the nitrocellulose membrane, the detection area is sprayed with acetamiprid hapten-carrier protein conjugate, the quality control area is sprayed with goat anti-mouse anti-antibody, and the acetamiprid hapten is made of E - N ‑(4‑(6‑chloropyridine‑3‑yl)2‑butene‑2‑yl)cyanamide reacts with aminopropionic acid. The invention also provides a preparation method of the test strip and a method for detecting acetamiprid in a sample by using the test strip. The test strip and detection method provided by the invention have the advantages of simple operation, high sensitivity, fast detection speed and low cost, and can realize rapid detection and on-site monitoring of acetamiprid in large batches of samples.

The invention relates to a preparation method and application of a double-photon fluorine ion fluorescent probe compound. The formula of the double-photon fluorine ion fluorescent probe compound is as shown in formula I. The preparation method includes: allowing 2, 4-dihydroxy benzaldehyde and cyclohexenone to react under nitrogen protection by using imidazole as the catalyst; allowing the product obtained by separation to react with tert-butyldimethylsilyl chloride to obtain the pure fluorine ion fluorescent probe compound. The fluorine ion fluorescent probe compound has the advantages that the probe compound has good selectivity and sensitivity to fluorine ions and is nontoxic to cells; probe molecules can be stimulated by double photons to avoid an ultraviolet region, so that the probe molecules are applicable to detection of the fluorine ions in water and detection and imaging of the fluorine ions in cells.

The invention discloses a time-resolved fluorescent immunochromatographic test strip for detecting iprodione, a preparation method and application thereof. The test strip includes a bottom plate and a sample absorption pad, a conjugate release pad, a nitrocellulose membrane, and a water absorption pad that are sequentially overlapped and pasted on the bottom plate. The conjugate release pad is embedded with iprodione monoclonal antibody labeled with fluorescent microspheres. , a detection area and a quality control area are fixed on the nitrocellulose membrane, the detection area is sprayed with iprodione hapten-carrier protein conjugate, the quality control area is sprayed with goat anti-mouse anti-antibody, and the iprodione hapten is composed of 3 , 5-dichlorophenyl isocyanate and glycine ethyl ester hydrochloride, 6-aminocaproic acid methyl ester hydrochloride, etc. are obtained through a series of reactions. The test strip and the detection method provided by the invention have the advantages of simple operation, high sensitivity, fast detection speed and low cost, and can realize rapid detection of iprodione in a large number of samples.