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Time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and preparation method and application thereof

A technology of time-resolved fluorescence and immunochromatography test paper, which is applied in the fields of analytical materials, biological testing, fluorescence/phosphorescence, etc., to achieve the effects of fast release, strong specificity, and reduced fluorescence intensity

Inactive Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no time-resolved fluorescent immunochromatographic test strip for the detection of carbendazim in tobacco and tobacco products

Method used

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  • Time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and preparation method and application thereof
  • Time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Composition of time-resolved fluorescent immunochromatographic test strips for detecting carbendazim

[0041] 1. Test strips

[0042] see figure 1 : The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0043] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[0044]The n...

Embodiment 2

[0046] Example 2 Preparation of time-resolved fluorescent immunochromatographic test strips for detecting carbendazim

[0047] The preparation method of the time-resolved fluorescent immunochromatographic test strip for detecting carbendazim mainly includes the following steps:

[0048] 1) Preparation of the conjugate release pad: Label the carbendazim monoclonal antibody with commercially available fluorescent microspheres and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and freeze-dry it in vacuum Post-preparation;

[0049] 2) Preparation of nitrocellulose membrane: Spray the carbendazim hapten-carrier protein conjugate on the detection area of ​​the nitrocellulose membrane to make the detection area; spray goat anti-mouse anti-antibody onto the nitrocellulose membrane The scope of the quality control area above is made into the quality control area;

[0050] 3) Assembly and cutting: Paste the sample absorption pad, the co...

Embodiment 3

[0109] Example 3 Application of time-resolved fluorescent immunochromatographic test strips for detecting carbendazim

[0110] 1. Tobacco sample pretreatment

[0111] Weigh 1.0±0.05 g crushed tobacco leaf sample into a polystyrene centrifuge tube; add 10 mL of 50% methanol aqueous solution, and use a homogenizer to fully break it up to obtain a sample liquid; pipette 100 μL of sample liquid and 400 μL to The ionized water was mixed and waited for inspection.

[0112] 2. Test with test strips

[0113] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be tested through the touch screen. item, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test results on the display scr...

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Abstract

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate and further comprises a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad which are lapped and pasted on the bottom plate in sequence. A carbendazim monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A carbendazim hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the qualitycontrol area. A carbendazim hapten is obtained in the mode of reacting of 2-aminobenzimidazole with butanedioic anhydride. The invention further provides the preparation method of the test strip and amethod for detecting the carbendazim in samples by applying the test strip. The time resolution fluorescent immunochromatographic test strip and the detecting method provided by the invention have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the carbendazim in the large-batch samples can be detected quickly and monitored on site.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting carbendazim in tobacco and tobacco products, a preparation method and application thereof. Background technique [0002] Carbendazim (Carbendazim) is a benzimidazole fungicide. It is a good broad-spectrum and systemic fungicide. It is effective against most pathogenic bacteria in Ascomycetes, Basidiomycetes and half-knowledge fungi. It is widely used in Disease control of crops, Chinese herbal medicine, tobacco, etc. Carbendazim has stable chemical properties and can be absorbed by seeds, roots and leaves of plants. It has a long residual effect period and has certain toxicity to humans and animals. For different crops, my country has formulated the maximum residue limit standard of carbendazim, among which, the maximum residue limit of carbendazim in grains is between 0.05 - 2 mg / kg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭赵乐崔华鹏樊美娟潘立宁颜权平王昇
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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