The invention relates to a tertiary butyl-containing spirobifluorene compound and preparation and application thereof. The material has the following general formula (I) shown in the description, wherein R is a structure shown in the description; -Ar shown in the description, wherein -Ar is selected from at least one of the following structural units or derivatives of the following structural units: shown in the description; in the above mentioned structural formulas, m and n are integers of 0 to 8, and R1, R2, R3 and R4 are hydrogen, or straight chains or branched chains or ring alkyl or oxyalkyl chains with 1 to 22 carbon atoms, and can be the same or different. Compared with the prior art, the tertiary butyl-containing spirobifluorene compound disclosed by the invention has the advantages that 4,4'-ditert-butyl-2-brominated biphenyl with low cost replaces 2-brominated biphenyl which is generally used in the conventional 9,9'-spirobifluorene preparation process and is high in cost, and the synthesis method has the characteristics of low cost, simple synthesis route, high yield, easiness in industrial production and the like; a spirobifluorene hole-transmission / electroluminescent material is high in hole-transmission performance and electroluminescent performance.