The invention provides a method for preparing tobacco perfume by
pile-fermenting tobacco stems by utilizing resting cells.
Aroma-producing strains separated from the surfaces of tobacco leaves are amplified and cultured in a luria-bertani (LB)
liquid culture medium and then centrifugally collected for standby application; a
phosphate buffer solution is compounded, and the concentration of the
buffer solution is controlled within a range that the
osmotic pressure of the
buffer solution can satisfy the requirement for
cell growth; all the
aroma-producing strains are grafted to the
phosphate buffer solution and cultured for 20 to 72 hours at a temperature of 20 to 37 DEG C, and then the resting cells are manufactured for standby application; 2 to 5 times of water is added to the tobacco stems to sterilize the tobacco stems, and the resting cells are inoculated with pathogens at a proportion of 0.5 to 10 percent of the total weight of the sterilized tobacco stems; the tobacco stems and the resting cells are evenly mixed, and
pile-fermented and cultured at a temperature of 20 to 37 DEG C for 24 to 120 hours after the resting cells are inoculated with the pathogens, and then a
fermentation product is obtained; and finally, water is added to the
fermentation product, the
fermentation product is heated, extracted and concentrated, and then the tobacco perfume is manufactured. By adopting the method,
wood gas in tobacco stem extracts can be effectively reduced and sweet
aroma can be supplemented, so that the smoking quality of the tobacco stem extracts can be improved, the originalaroma of tobacco can be effectively promoted, stimulation can be reduced and the richness of the tobacco stem extracts can be increased.