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Natural vanillin composite perfume and uses thereof

A technology of vanillin and flavorings, applied in the field of applied microorganisms, can solve the problems of undiscovered public reports, etc., and achieve the effects of improving cigarette smoking taste, delicate alcohol and smoke, and enhancing cigarette aroma

Inactive Publication Date: 2010-04-21
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But through document retrieval, do not find the public report of identical document with the content of the present invention

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  • Natural vanillin composite perfume and uses thereof
  • Natural vanillin composite perfume and uses thereof

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Embodiment Construction

[0015] The following are examples of the present invention, but the content of the present invention is not limited thereto.

[0016] 1: Preparation of vanillin compound flavor

[0017] The present invention is achieved like this:

[0018] Culture method of Enterobacter sp.Px6-4:

[0019] 1. Test tube slant culture

[0020] A Luria-Bertani (LB) medium is used, and the formula is: 1% peptone, 1% sodium chloride, 0.5% yeast extract, and 1.8-2% agar. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Enterobacter sp.Px6-4 and culture at 37°C for 18-24 hours to obtain test tube seeds.

[0021] 2. Liquid expansion culture

[0022] Using Luria-Bertani (LB) medium without adding agar, aliquot 3-5 in a 18×180mm test tube

[0023] mL of liquid LB medium, sterilized, inoculated with Enterobacter sp.Px6-4, cultured at 37°C for 18-24 hours to obtain liquid strains.

[0024] 3. Liquid fermentation culture

[0025] The components of the fermentation medium are: feruli...

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Abstract

The invention relates to a natural vanillin composite aroma material and application thereof, pertaining to the microbial application technical field. The aroma can be obtained from producing strain and auxiliary materials after an amplification culture of liquid, liquid fermentation and extracting separation; wherein the producing strain thereof belongs to Enterobacter sp.Px6-4 and the strain hasbeen preserved at CGMCC since April 12, 2007 with the preserving number of CGMCC No. 1999. The aroma substance of vanillin composite aroma materials and the corresponding percentage content respectively are: vaniilin 0.69%, 4-ethyl group guaialcol 0.75%, 4-vinyl guaialcol 70.31%, 4-acetyl guaialcol 0.08%, 4-propyl guaialcol 0.09%, 4-guaialcol 1.63%, 1-butyl alcohol 0.34%, 3-hydroxyl groups-2-butanone 0.05%, unknown material with undetermined structure 6.34%. The vanillin composite aroma material is suitable for perfume cigarette application. The vanillin composite aroma material of the invention has the advantages of well fitting to the cigarette branch, improving cigarette fragrance evidently, refining alcohol and smoke, and ameliorating sucking flavor, etc.

Description

Technical field: [0001] The invention relates to a natural vanillin compound fragrance, which belongs to the technical field of applied microorganisms. Background technique: [0002] Vanillin (vanillin) is the main component of vanilla beans, also known as vanillin, vanillin, etc., the chemical name is 3-methoxy-4-hydroxybenzaldehyde (3-methoxy-4-hydroxybenzaldehyde), The relative molecular weight is 152.15, white to light yellow crystalline powder or needle crystal. Vanillin exists in the plant in free form or in the form of glycosides, accounting for 2%-3% of the dry weight of vanilla beans. Pure vanillin has a strong milky aroma and no peculiar smell. Vanillin is an important spice substance, widely used in food, beverage, spice and medicine and other fields. About 60% are used as fragrance substances in food, candy and beverages, about 33% are used as ingredients in spices and cosmetics, and about 7% are used as pharmaceutical intermediates (Wang Duoren 2000). [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B9/00C12P7/24C12R1/01
Inventor 杨金奎李雪梅张克勤顾雯
Owner YUNNAN UNIV
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