The invention discloses a method for efficiently and quickly extracting
crop genome DNA (DeoxyriboNucleic Acid). The method specifically comprises the following steps: putting a
root system, a
seedling or a leaf which grows after the single seed of a
crop sprouts into a container, and adding an alkaline solution and steel balls to carry out
grinding pyrolysis so as to obtain homogenate; adding
Tris buffered solution into obtained lysate, and collecting upper-layer clear solution. Compared with a traditional alkaline
pyrolysis method, the method disclosed by the invention is characterized in that TritonX-100 solution or NP40 solution are added in an alkaline
grinding pyrolysis process to more simply and quickly extract
DNA, extraction quality is higher, and an extraction effect is better. Since the
Tris buffered solution is added, an influence for subsequent PCR (
Polymerase Chain Reaction) since the pH (Potential of
Hydrogen) value of the extracted
DNA solution is overhigh or overlow can be avoided, and only two solutions need to be added to extract high-quality DNA; a high-temperature water bath process is omitted, and the efficient extraction of the DNA can be realized only through two steps. The method established by the invention is suitable for the quick detection of the purity, the authenticity and the transgenic ingredients of the single seed of the
crop and the auxiliary selection research of the key character
molecular marker of the crop.