38results about How to "Applicable quality control" patented technology

The invention discloses a HPLC-ELSD content determination method for paeoniflorin and albiflori in red paeony roots. The method includes the steps that 1, a test product solution is prepared; 2, comparison product solutions are prepared; 3, detection is performed, namely, the paeoniflorin comparison product solution and the albiflori comparison product solution in the step2 are taken to be detected under the detection conditions of C18 columns, isocratic elution is performed with an acetonitrile-0.05% formic acid aqueous solution as a mobile phase, HPLC-ELSD detection is performed through an ELSD detector, and curves are drawn to obtain standard curve equations; HPLC-ELSD detection is performed on the test product solution in the step1 under the conditions through the ELSD detector, obtained peak areas of the paeoniflorin and the albiflori are substituted into the standard curve linear regression equations, and then the contents of the paeoniflorin and the albiflori in the red paeony roots can be obtained. The method is good in specificity, durability, precision and accuracy and suitable for global quality control over the Chinese herb-red paeony roots.

This invention relates to compound thrombus-relaxing agent fingerprint spectrum recreation method by use of natural plants medicinal materials, which comprises the following parts: test solution process; comparing liquid process; measuring with effective liquid chromatograph measurement with chromatograph relative keeping time and peak area as one; computing the test items relative keeping time and relative peak area to get the HPLC fingerprint chromatograph. The required HPLC standard fingerprint chromatograph to notoginseng chromatograph has five common peaks; the one to astragalus root hastwo common peaks; chromatograph to radix salvia miltiorrhiza has two common peaks; one to scrophularia root has two common peaks; the one to radix salvia miltiorrhiza under 270 nm has three common peaks; the one to scrophularia root has common peak.

The invention discloses a method for detecting the fingerprint of anti-lung cancer sea mulberry recipe, comprising the following steps: step 1: preparation of a reference peak solution; step 2: preparation of a test solution of anti-lung cancer ha mu recipe; step 3: separately precise Inject the quantitative reference peak solution and the test solution into the liquid chromatograph, and record the chromatogram; Step 4: Export the Haimu Fang fingerprint instrument obtained in step 3, and import it into the Chinese medicine chromatographic fingerprint similarity evaluation system, select different The chromatographic peaks that exist in the chromatograms of the batches of Haimufang tested are regarded as common peaks, and the average value calculation method is used to generate the control fingerprint of Haimufang, and the relative retention time and relative peak area of ​​each common peak are calculated. The fingerprint spectrum of the Haimu prescription provided by the invention can comprehensively and objectively characterize the quality of the Haimu prescription, and is beneficial to comprehensively monitor the quality of the medicine. The fingerprint detection method provided by the invention has the advantages of simple method, stability, high precision, good reproducibility and the like.

The invention belongs to the field of medicinal material detection, and in particular relates to a quality detection method for Yupingfeng particles. The quality detection of the Yupingfeng particlescomprises character detection, thin-layer chromatography detection, fingerprint spectrum detection and content limitation detection. The quality detection method for the Yupingfeng particles providedby the invention can authenticate two traditional Chinese medicines: Astragalus membranaceus and Radix saposhnikoviae at a time via a method by using thin-layer chromatography, and is high in accuracyand strong in practicability; the fingerprint spectrum and component content of the Yupingfeng particles can be measured in a unified manner by using ultra-high performance liquid chromatography; viatechnological investigation, the content measurement method provided by the invention is high in accuracy, good in stability and excellent in repeatability, significantly reduces analysis and detection time, and has great significance in setting of Yupingfeng particle quality standard.

The invention discloses an ion chromatography method for simultaneously detecting mepiquat as a main component and N-methylpiperidine as an impurity in a pesticide. The ion chromatography method comprises the following steps of (1) setting the temperature of a column oven to be 45 DEG C; (2) setting the sample injection amount to be 25 microliters; (3) setting applied suppression current to be 15mA; (4) preparing leacheate; (5) preparing a standard solution; (6) analyzing a mixed standard solution with the N-methylpiperidine and the mepiquat; (7) diluting a liquid sample by 10,000 times as a sample solution for later use; (8) filtering a post-treated sample through a disposable needle type filter, putting the sample into an ion chromatography for analysis to measure the contents of negative N-methylpiperidine and mepiquat ions. The method disclosed by the invention is quick, simple and accurate, is suitable for quality control in pesticide production and can meet a requirement on technical control in a pesticide production process.

The invention provides a method for measuring the content of 4,4'-bi(hydroxymethyl) biphenyl (4,4'-BHMBP). In the method, a capillary gas-phase chromatographic column is used for separating the 4,4'-bi(hydroxymethyl) biphenyl, and an automatic sample injector is used for quantifying the 4,4'-bi(hydroxymethyl) biphenyl by adopting an external standard calibration curve method. The method is characterized in that a fixed phase of the capillary gas-phase chromatographic column is 5 percent phenyl 95 percent polydimethylsiloxane, the length of the column is 15-60m, the inner diameter of the column is 0.2-0.5mm, the film thickness of the inner diameter of the column is 0.1-0.6mum, the initial temperature of the column is 60-150 DEG C, and the heating rate is 4-10 DEG C / min; and the sample size of the automatic sample injector is 0.2-5mul, the split ratio is (5:1)-(50:1), and the vaporization temperature is 230-270 DEG C. The method is simple, quick and accurate, is a more ideal qualitative and quantitative analysis method for measuring the content of the 4,4'-BHMBP and is suitable for the quality monitoring of 4,4'-BHMBP products.