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A kind of detection method of blood-nourishing brain preparation

A technology for nourishing blood and brain and detection methods, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of long analysis time, small proportion of ingredients, quantitative determination of paeoniflorin, etc., and achieve large quantity and polar coverage big effect

Active Publication Date: 2022-02-18
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, Document 1 has the following defects: (1) The analysis time is too long (90 minutes), which is not conducive to the quality evaluation and control of Yangxue Xuenao Granules in actual production; (2) Due to the limited monitoring wavelength (327nm, 280nm), The content of selected quantitative components is low (respectively ferulic acid, caffeic acid, rosmarinic acid, baicalin); the method does not carry out quantitative determination of paeoniflorin with a large content (generally the detection wavelength is 230-240nm); (3 ) does not specify the evaluation method used for quality control
[0007] Document 2 has the following defects: ① Only qualitative analysis is carried out for volatile components, and there is no quantitative monitoring of components, which can be used for qualitative identification of products
②A total of 10 common peaks were identified in this study. Except for a relatively large component peak (Z-ligustilide), the proportion of each component is relatively small, and it only comes from two medicinal materials, Angelica and Chuanxiong, and cannot be used in Representative map of the overall quality evaluation of this product

Method used

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  • A kind of detection method of blood-nourishing brain preparation
  • A kind of detection method of blood-nourishing brain preparation
  • A kind of detection method of blood-nourishing brain preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0183] 1. Preparation of reference solution

[0184] Take an appropriate amount of paeoniflorin reference substance, weigh it accurately, add 70% ethanol to make a solution with a concentration of 0.05 mg / ml, shake well, and obtain the paeoniflorin reference substance solution;

[0185] Take an appropriate amount of the orange-yellow cassiatin reference substance, accurately weigh it, add 70% ethanol to make a solution with a concentration of 5.0 μg / ml, shake well, and obtain the orange-yellow cassiatin reference substance solution.

[0186] 2. Preparation of the test solution Take about 0.4 g of the serum brain granule, accurately weigh it into a stoppered Erlenmeyer flask, add 15 ml of 70% ethanol precisely, and ultrasonically treat it for 15 minutes, let it cool and then centrifuge (3000 rpm ) for 5 minutes, accurately draw 5ml of the supernatant, pass through the treated DIKMA PLS solid-phase extraction column (500mg, first pre-wash with 10ml of acetonitrile, then pre-wash...

Embodiment 2

[0194] 1. Preparation of reference solution

[0195] Take an appropriate amount of paeoniflorin reference substance, weigh it accurately, add 70% ethanol to make a solution with a concentration of 0.01 mg / ml, shake well, and obtain the paeoniflorin reference substance solution;

[0196] Take an appropriate amount of the orange-yellow cassiatin reference substance, accurately weigh it, add 70% ethanol to make a solution with a concentration of 1.5 μg / ml, shake well, and obtain the orange-yellow cassiatin reference substance solution.

[0197] 2. Preparation of the test solution Take about 0.4g of the serum brain granule, accurately weigh it into a stoppered Erlenmeyer flask, add 10ml of 70% ethanol precisely, and ultrasonically treat it for 20 minutes, let it cool and then centrifuge (3000 rpm ) for 5 minutes, accurately draw 5ml of the supernatant, pass through the treated DIKMA PLS solid-phase extraction column (500mg, first pre-wash with 10ml of acetonitrile, then pre-wash w...

Embodiment 3

[0205] 1. Preparation of reference solution

[0206] Take an appropriate amount of paeoniflorin reference substance, weigh it accurately, add 70% ethanol to make a solution with a concentration of 0.10 mg / ml, shake well, and obtain the paeoniflorin reference substance solution;

[0207] Take an appropriate amount of the orange-yellow cassiatin reference substance, accurately weigh it, add 70% ethanol to make a solution with a concentration of 20 μg / ml, and shake well to obtain the orange-yellow cassiatin reference substance solution.

[0208] 2. Preparation of the test solution Take about 0.3 g of Yangxue Xuenao Pills, accurately weigh it into a stoppered Erlenmeyer flask, add 25 ml of 70% ethanol precisely, and process it with supersonication for 25 minutes, let it cool and then centrifuge (3000 rpm ) for 5 minutes, accurately draw 5ml of the supernatant, pass through the treated DIKMA PLS solid-phase extraction column (500mg, first pre-wash with 10ml of acetonitrile, then pr...

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Abstract

The present invention relates to a detection method of nourishing serum and brain preparation, said method is high-performance liquid chromatography, comprising the following steps: step 1, preparation of test solution, step 2, preparation of reference solution, step 3, chromatography Condition; Step 4, assay, need testing solution and reference substance solution are injected high-performance liquid chromatograph, obtain chromatogram. In the method provided by the present invention, after the test sample is processed by a solid-phase chromatographic column, the components are analyzed after enrichment and purification, so that the analysis time of the corresponding chromatographic method is shorter, and at the same time, the separation of chromatographic peaks and the weight of the method are guaranteed. and the present invention clearly defines the relative quantitative mode of the fingerprint, and clearly defines the control requirements of the absolute quantitative index (2) of the content determination and the relative quantitative index (19) of the fingerprint, and the control index is more comprehensive, to The quality attributes of the product are described more quantitatively.

Description

Technical field: [0001] The invention relates to a detection method of a traditional Chinese medicine preparation, in particular to a detection method of a blood-nourishing brain preparation. Background technique: [0002] Blood-nourishing brain preparations (including pills, granules, etc.) are made of eleven traditional Chinese medicines and auxiliary materials such as angelica, Chuanxiong, white peony root, rehmannia glutinosa, Uncaria, Millae, Prunella vulgaris, cassia seed, mother of pearl, corydalis, and asarum Chinese patent medicines are currently on the market. The preparation of nourishing serum and brain has the effects of nourishing blood, calming the liver, promoting blood circulation and dredging collaterals. It can be used for headache, dizziness, irritability, insomnia and dreaminess caused by blood deficiency and hyperactivity of the liver, and has a significant clinical curative effect. It can improve pia mater microcirculation, increase cerebral blood flo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 葛丹丹苏娟李芳明高展孙玉侠
Owner TIANJIN TASLY PHARMA CO LTD
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