766 results about "CMC chromatography" patented technology

This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

In the present capillary electrokinetic chromatograpy method, analytes are injected by electroosmotic flow directly from a sample matrix into a separation buffer containing an electrokinetic vector with an opposite mobility. Analytes can now be injected at the velocity of electroosmotic flow, but are retained at the interface of the sample matrix-co-ion and separation buffer micelle zones as analyte / micelle complexes. Manipulation of the injecting force and opposing stacking force allow greatly increased length or volume of injection. Concentrations of the micelle, methanol, and borate in the separation buffer were provided to increase maximum injection length of neutral analytes. Reducing the analyte velocity in the separation buffer without substantially decreasing the velocity of the analyte during injection from the sample vial allowed greatly extended sample plug injection lengths. It is further enabled to inject sample solvent volumes equivalent to about twenty times the effective capillary volume. Equations and algorithms describing the injection process and maximum injection lengths for this mode of stacking in electrokinetic capillary chromatography are introduced. Use of the present method provides for maximum electrokinetic stacking injection for a wide variety of analytes and separation systems.

This invention provides safflower uranidin prepd. from the Chinese herb medicine-safflower which isu sed for promoting blood circulation and removing blood disturbance, and the prepn. method and usage therefor. Said safflower uranidin is tested by high efficiency liquid phase chromatography with its content of 70-99.6%. It can be used for treating and preventing various kinds of diseases, such as cardiovascular disease, cerebrovascular disease and other blood circulation disturbance disease.

Carbon nanotubes dispersed by a stabilized solution of nucleic acid molecules formed separated nanotube-nucleic acid complexes and were separated according to standard chromatographic methods, including gel electrophoresis, two phase solvent systems and ion exchange chromatography.

The object of the invention is a multiple-channel test devise based on immunodiffusion and immunochromatography, which enables the simultaneous or parallel determination of several analytes. In the test devise, it is possible to group together different combinations of markers recognizing allergens, myocardial infarction markers, venereal disease analytes, blood screening analytes, respiratory infection producing agents, IgG, IgA and IgM antibodies, other infectious disease producing agents as well as various cancer markers. The multiple-channel test devise comprises a porous carrier material on which a channel network has been formed by etching the carrier material by laser to form a shaped figure that contains several channels. In the channels, various specific binding reagents have been immobilized, which enable the diagnoses of a target illness and / or syndrome. The sample application point is optionally provided with a filter and optionally contains a label mobilizable by the analyzable sample and a specific binding reagent. Also the method for the production of the test device and its use are disclosed in the invention.

The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.

The invention discloses a quality testing method for fingerprint of herbal medicine musk. The method includes establishing a gas chromatographic fingerprint of the musk by gas chromatography, and analyzing chemical components of different types of musk by gas chromatography and mass spectrometry. A test sample is processed by specific process, chromatographic detection is optimized, and tests show that the method is stable, precise and repeatable. Three established reference musk fingerprints are well specific. Similarity of the three types of musk is researched under integration of non-integration conditions of muscone, and more accurate basis is provided for testing quality of the three different types of musk. In addition, common compounds of the three types of musk are obtained by identification by GC-MS (gas chromatography and mass spectrometry) and can be used as basis for further identification of synthetic musk, domestic musk and natural musk.

Method for purifying a racemized polypeptide by ion-exchange chromatography.

The invention discloses a method for controlling the quality of the Longdanxiegan Capsule. The invention adopts the combined methods of character and thin layer chromatography discrimination and high efficiency liquid chromatography content measurement so as to control the quality of the Longdanxiegan Capsule, wherein during the operation of thin layer chromatography discrimination, characteristic spots of bupleurum, radix scutellariae, scutelloside, ferulic acid, glycyrrhiza and ammonium glycyrrhetate are respectively detected, the content of gentiamarin and geniposide is detected simultaneously by the high efficiency liquid chromatography under the condition of identical chromatogram through the mode of gradient elution, the content of the gentianella of each capsule is more than 0.07 mg according to gentiamarin, and the content of the gardenia of each capsule is more than 1.0 mg according to geniposide. The method respectively carries out the operation of thin layer chromatography discrimination on the characteristic components of the Longdanxiegan Capsule, can scientifically and comprehensively reflect the existence of monarch drugs, ministerial drugs, adjuvant and conductant drugs in the Longdanxiegan Capsule, simultaneously detects the content of the characteristic components of the gentianella and the gardenia; moreover, the method is simple, scientific and easy to operate, and is conducive to the comprehensive quality control of the Longdanxiegan Capsule.