HPLC-ELSD content determination method for paeoniflorin and albiflori in red paeony roots

Abstract

The invention discloses a HPLC-ELSD content determination method for paeoniflorin and albiflori in red paeony roots. The method includes the steps that 1, a test product solution is prepared; 2, comparison product solutions are prepared; 3, detection is performed, namely, the paeoniflorin comparison product solution and the albiflori comparison product solution in the step2 are taken to be detected under the detection conditions of C18 columns, isocratic elution is performed with an acetonitrile-0.05% formic acid aqueous solution as a mobile phase, HPLC-ELSD detection is performed through an ELSD detector, and curves are drawn to obtain standard curve equations; HPLC-ELSD detection is performed on the test product solution in the step1 under the conditions through the ELSD detector, obtained peak areas of the paeoniflorin and the albiflori are substituted into the standard curve linear regression equations, and then the contents of the paeoniflorin and the albiflori in the red paeony roots can be obtained. The method is good in specificity, durability, precision and accuracy and suitable for global quality control over the Chinese herb-red paeony roots.

Description

technical field [0001] The invention relates to an HPLC-ELSD content determination method of paeoniflorin and paeonifloride in red peony, belonging to the field of biomedicine. Background technique [0002] Radix Paeoniae Rubra is the dry root of Paeonia actiflora Pall. or Paeoniaveitchii Lynch, a plant of Ranunculaceae, which has a long history of medicinal use. In recent years, studies have found that Radix Paeoniae Rubra has a wide range of pharmacological effects such as anticoagulant, antithrombotic, anti-atherosclerosis, protection of the heart and liver, and protection of brain damage caused by cerebral ischemia, and has broad application prospects. [0003] The main active ingredients of Radix Paeoniae Rubra are paeoniflorin, paeoniflorin, hydroxypaeoniflorin, benzoylpaeoniflorin and other monoterpene glycosides, collectively known as total glycosides of paeoniflorin, of which paeoniflorin and paeoniflorin account for about 70% of the total glycosides. %. Paeoniflo...

AI Technical Summary

Problems solved by technology

Most researchers use high-performance liquid chromatography (DAD detector) to evaluate the quality of Radix Paeoniae Rubra. DAD detectors require substances to have ultraviolet emitting groups, such as glycosides such as paeoniflorin and paeonifloride glycosides in Radix Paeoniae Rubra The ultraviolet emitting groups of the two substances are in the near-ultraviolet region, and the molar absorptivity is small, and the use of DAD has certain restrictions on the quantification of these two substances

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