The invention provides a restored protein peptide nutritional supplement and a preparation method thereof. The restored protein peptide nutritional supplement includes the following main raw materials: soybean protein isolate powder (protein content >= 90%), fish collagen powder, oat powder, polydextrose (soluble dietary fiber), phospholipid, black soybean powder, red bean powder, Agaricus blazeipowder, xylo-oligosaccharide, methylotrophic yeast, common yam rhizome powder, Hericium erinaceus powder, blueberry powder, white tremella powder, Agrocybe aegerita powder, beta-carotene, tea polyphenols, carboxymethyl cellulose sodium, edible essence, ethyl maltol, acesulfame potassium and aspartame (containing phenylalanine). The invention adopts selected raw materials to be baked at low temperature, so as to protect nutritional components from being destroyed to the greatest extent. The restored protein peptide nutritional supplement is prepared according to the theory of medicine and foodhomology and the theory of traditional Chinese medicine, so that the restored protein peptide nutritional supplement can improve human immunity, increase gastrointestinal motility and remove dampnessand dispel toxin, so as to achieve the purpose of comprehensive regulation; and the restored protein peptide nutritional supplement is free of toxic or side effects, and can be used for a long time.