The invention provides a printing unit of a screen printing machine. The printing unit comprise a rack, a screen frame, a screen bracket, a roller and a printing cutter bit, wherein a rear supporting plate of the screen bracket is provided with a screen adjusting and locking device; four corners of the screen bracket are respectively provided with a screen clamping device; the printing cutter bit comprises a scraping and printing cutter, a cutter frame arranged on the scraping and printing cutter, a cutter bit box arranged on the cutter frame, a scraping and printing cutter pressurizing device, a scraping and printing cutter angle adjusting and locking device, a scraping and printing cutter position adjusting device and a scraping and printing cutter switching device. According to the printing unit provided by the invention, by adopting an oblique table type screen frame, the screen bracket only needs to be obliquely lifted by hands when screen cloth needs to be cleaned, the screen bracket can be supported by an air spring, and thus the screen cloth can be more simply and conveniently cleaned; meanwhile, a closed oil way is formed by a decompression valve, an oil storage seat, an oil cylinder, an oil valve seat and the like, a control air way is formed by an external air source and the decompression valve, thus the cutter frame is controlled, and the stability of the cutter frame is greatly increased.