37 results about "Tumour invasion" patented technology

The invention provides an application of shiandra and dibenzocyclooctadiene lignans for preparing medicaments for resisting tumor invasion and metastasis. When the dibenzocyclooctadiene lignans are used for preparing medicaments for resisting tumor invasion and metastasis, a mixture of two or more than two compounds can also be adopted. In the invention, the application of the shiandra and dibenzocyclooctadiene lignans for preparing medicaments for resisting tumor invasion and metastasis has the following beneficial effects: the shiandra and dibenzocyclooctadiene lignans can effectively prevent and control tumor invasion and metastasis and have good clinical application prospects.

The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.

A method of identifying transcription factors comprising providing cells with a nucleic acid sequence at least comprising a sequence CACCT (SEQ ID NO: ______ ) as bait for the screening of a library encoding potential transcription factors and performing a specificity test to isolate said factors. Preferably, the bait comprises twice the CACCT (SEQ ID NO: ______ ) sequence, more particularly the bait comprises one of the sequences CACCT-N-CACCT(SEQ ID NO: ______ ), CACCT-N-AGGTG(SEQ ID NO: ______ ), AGGTG-N-CACCT(SEQ ID NO: ______ ), or AGGTG-N-AGGTG (SEQ ID NO: ______ ) wherein N is a spacer sequence. The transcription factors identified using the methods of the invention include separated clusters of zinc fingers, such as, for example, a two-handed zinc finger transcription factor. Also, at least one such zinc finger transcription factor, denominated as SIP1, induces tumor metastasis by down regulation of the expression of E-cadherin. Compounds interfering with SIP1 activity can thus be used to prevent tumor invasion and metastasis.

The invention relates to trehalose derivatives with general formula (I), a preparation method and uses thereof, wherein 6,6′-bis(2,3-dimethoxybenzoyl)-α,α-D-trehalose has anti-colon cancer 26-L5 cell invasion activity which is better than that of a natural product Brartemicin, IC50 is 0.10 μg / mL (0.15 μM), and when the IC50 is 10 μg / mL, 6,6′-bis(2,3-dimethoxybenzoyl)-α,α-D-trehalose has no cytotoxicity, shows high-selectivity anti-tumor invasion activity and can be used for preparing medicaments for preventing and treating invasion and metastasis of colon cancer and the like.

The invention belongs to the biomedical engineering field, and relates to a trachea substitute and a preparation method therefor. The trachea substitute has a porous and three-dimensional network structure, is a tubular, tegular or Y-type decellularized biomaterial, and comprises biological materials of allogeneic or heterogeneous sources. There are holes on the outer surface and the outer surface is seamed with spiral polyester materials. Experiments show that the trachea substitute has good biocompatibility with tissues, has no antigen interference effects, can perform revascularization and re-epithelization rapidly, has good air impermeability, ductility and elasticity, and can prevent collapse during breath. The trachea substitute can be made into different shapes and specifications, is suitable for tracheal reconstructive operations of tubular trachea substitution, juga molding, tracheal patching and the like. The trachea substitute is especially suitable for post-operation trachea substitution of trachea tumours, trachea trauma, benign tracheal stenosis, tumour invasion, congenital trachea malformation and the like.

The invention provides methods for treating HIF-1α-overexpressing human tumors, inhibiting HIF-1α-overexpressing tumor invasion and preventing tumor metastasis, and / or promoting tumor prophylaxis, using various types of inhibitors against the Hsp90α from the tumors.

The invention provides a TIPE3 immunohistochemical detection kit as well as a use method and application thereof, and belongs to the technical field of tumor detection. The research finds that the expression of the TIPE3 in the pancreatic cancer is obviously increased, is closely related to tumor invasion and metastasis potential and is an independent prognosis risk factor of a pancreatic cancer patient, so that the aims of helping to judge the malignant degree of the pancreatic cancer and prognosis of the patient can be fulfilled by detecting the expression of the TIPE3, and the TIPE3 immunohistochemical detection kit is provided. The kit can effectively predict the malignancy degree and metastasis potential of pancreatic cancer, and perform recurrence and metastasis risk grading on pancreatic cancer patients; and meanwhile, the prognosis condition of the patient can be evaluated, so that the survival rate of the pancreatic cancer patient can be improved, an important scientific basis is provided for clinical prevention and treatment of the pancreatic cancer, and therefore, the kit has good clinical application value and social benefits.

The invention relates to multiple-target fusion protein resistant to tumor invasion and metastasis. The fusion protein has following amino acid sequence structures: RGD peptide-connecting peptide-TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) functional peptides-connecting petide-NGR peptide; the fusion protein has proliferation effect on inhibiting cancer cells of the TRAIL peptides and migration inhibition of the tumor cells of two targeting peptides of GRD and NGR. By comparison, the multiple-target fusion protein is more remarkable in antitumor activity, and resistance to the TRAIL is reversed to a certain extent. The invention further provides an encoding gene of the fusion protein and a pharmaceutical purpose of the fusion protein.

A method of identifying transcription factors comprising providing cells with a nucleic acid sequence at least comprising a sequence CACCT (SEQ ID NO:1) as bait for the screening of a library encoding potential transcription factors and performing a specificity test to isolate said factors. Preferably, the bait comprises twice the CACCT (SEQ ID NO:1) sequence, more particularly the bait comprises one of the sequences CACCT-N-CACCT (a first SEQ ID NO:1 and a second SEQ ID NO:1 separated by N), CACCT-N-AGGTG (SEQ ID NO:1 and SEQ ID NO:3 separated by N), AGGTG-N-CACCT (SEQ ID NO:3 and SEQ ID NO:1 separated by N), or AGGTG-N-AGGTG (a first SEQ ID NO:3 and a second SEQ ID NO:3 separated by N), wherein N is a spacer sequence. The transcription factors identified using the methods of the invention include separated clusters of zinc fingers, such as, for example, a two-handed zinc finger transcription factor. Also, at least one such zinc finger transcription factor, denominated as SIP1, induces tumor metastasis by down regulation of the expression of E-cadherin. Compounds interfering with SIP1 activity can thus be used to prevent tumor invasion and metastasis.

The invention provides a polypeptide-metal cluster probe for specifically recognizing membrane protein of a circulating tumor cell (CTC) and application of the polypeptide-metal cluster probe in quantitative detection, and belongs to the technical field of medical detection. The polypeptide-modified metal cluster probe prepared by the invention contains a metal cluster core and a target peptide which is specifically combined with target cell membrane protein. The prepared polypeptide-metal cluster probe targets the membrane protein expressed by CTC, and a laser ablation inductively coupled plasma mass spectrometry technology is utilized to perform rapid and accurate in-situ quantitative analysis on the peripheral blood CTC target membrane protein of a clinical tumor patient, so that an important reference is provided for tumor invasion and metastasis behaviors, grading and prognosis of the clinical tumor patient.

A preparation method and application of a double-regulated supramolecular assembly that inhibits tumor invasion and spread. Nano-supramolecular fiber aggregates constructed by supramolecular host-guest interactions. The advantages of the present invention are: the supramolecular assembly is oriented and aggregated under the induction of the geomagnetic field or a weak magnetic field, and can be light-controlled to induce the aggregation of the supramolecular assembly; on the other hand, the supramolecular assembly can specifically attract cancer cells, and the assembly can cause damage to mitochondria; the preparation method of the supramolecular assembly with dual regulation of magnetic field and light is simple, easy to implement and low in raw material cost, making it widely used in the field of tumor treatment, especially in Active inhibition of tumor cell invasion and spread has broad application prospects.

The invention provides an impedance sensing method for monitoring population cell invasion in a three-dimensional matrix. The method comprises the following steps: S1, preparing a conductive chip with a microelectrode array; s2, surrounding the periphery of the conductive chip with the annular structure to form a cell culture cavity; s3, performing surface treatment on the conductive chip; s4, fully paving cells on the microelectrode array of the cell culture cavity and forming polymeric hydrogel; s5, performing cell impedance detection in real time to obtain impedance information of the cells so as to obtain information of a cell invasion process. According to the invention, collagen I type gel suitable for a tumor invasion model is prepared on a microelectrode array conductive chip fully paved with cells through a self-assembly fibrosis process, and efficient and stable charge transfer in a cell impedance sensing detection process is effectively realized in combination with an impedance spectrometer on-line detection system; therefore, the technical effects of real-time, quantitative, label-free and whole-course dynamic analysis are achieved for detecting the invasion of the population cells in the three-dimensional matrix.

Disclosed herein is treating, reducing the incidence of one or more activities in or of cancer cells, or preventing one or more activities in or of cancer cells Methods of multiple activities, methods of treating, reducing the incidence of, or preventing migration or metastasis of cancer cells, by reducing tumor-associated macrophages (TAMs) or their migration Methods of treating cancer, reducing the incidence of cancer or preventing cancer, and treating cancer, including metastases, reducing the incidence of cancer, including metastases, for example using inhibitors of motile sperm domain-containing protein 2 (MOSPD2) or Methods of preventing cancer, including metastatic cancer. Also disclosed are inhibitors of MOSPD2 (eg, anti-MOSPD2 antibodies or antigen-binding fragments thereof) and pharmaceutical compositions containing MOSPD2 inhibitors. Also disclosed are methods for predicting, diagnosing, or prognosing cancer, cancer metastasis, tumor progression, or tumor invasion in a subject.

A recombinant fusion protein able to resist against tumor invasion and transfer is composed of the recombinant urokinase fragment and type-2 mutant as plasminogen activator inhibitor. The fusion protein gene cDNA is amplified by PCR method, and is cloned to expression vector to obtain fusion gene expression plasmid, which is used to transform host bacteria. The methanol or temp can induce the expression of said fusion protein. After purified, a high-purity (95%) expression product is obtained.

The invention discloses application of valproic acid in preparation of a multidrug resistance reversal agent of a lung cancer resistance medicine, and further discloses application of the valproic acid in the preparation of chemotherapeutics sensitizer of lung cancer resistance and a multidrug resistance tumor invasion resisting and transferring medicine. The application of the valproic acid in the preparation of the multidrug resistance reserval agent of the lung cancer resistance medicine verifies that valproic acid has significant function of a cisplatin persister of human lung cancer resistance through experiments, and verifies that the valproic acid can significantly change drug tolerance of a human lung cancer cell to the cisplatin, improve sensitivity of the human lung cancer cell to the cisplatin, and have obvious restraining effect of invasion effect of the persister of the lung cancer cisplatin.

The invention relates to a cholangiocarcinoma cell medicine target point. The medicine target point is ubiquitin specific protease 8, and the nucleotide sequence is shown as SEQID NO:1 in a sequence table. For the first time, the invention puts forward that USP8 is knocked down, so that proliferation, migration and invasion of cholangiocarcinoma cells can be notably restrained, MMP9 activity achieving important effects on cell adhesion, neoplasm invasiveness and transfer is reduced, and the USP8 is promoted to achieve effect of oncogene for growth and transfer ability of cholangiocarcinoma. New possibility is provided for development of cholangiocarcinoma medicines and cholangiocarcinoma prevention, treatment and prognosis.