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Trachea substitute and preparation method therefor

A substitute, tracheal technology, applied in the field of biomedical engineering, can solve problems that have not yet been seen, and achieve the effects of no antigen interference, enhanced collapse, and rapid re-epithelialization

Inactive Publication Date: 2014-02-19
SHANGHAI CHEST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there has been no report on the preparation of decellularized tracheal substitutes based on decellularized technology and using biologically derived tracheal tissue materials as raw materials

Method used

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  • Trachea substitute and preparation method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take the fresh tracheal tissue of the isolated sheep to prepare the sheep tracheal acellular matrix, remove the epithelial tissue and submucosa cell components, remove 70% of the chondrocyte components, and retain the trachea Extracellular matrix, made of porous three-dimensional network structure:

[0032] (1) The fresh tracheal tissue of sheep obtained by conventional treatment, the attached fat tissue was cut off, and placed in phosphate-buffered saline (phosphate-buffered saline, PBS) for transportation and preservation;

[0033](2) Put the obtained tracheal tissue into hypotonic tris buffer solution (hypotonic tris buffer, Ph8.0) containing protease inhibitor phenylmethyl-sulfonylfluoride (0.35ml / L) Medium, 4°C shaker mixing treatment for 24 hours;

[0034] (3) Add 1% mild detergent Triton to the above solution, continue to treat for 24 hours, and rinse thoroughly with Hank's simulated body fluid;

[0035] (4) Add DNase and treat with RANase at 37°C for 1 hour; ...

Embodiment 2

[0042] The porcine tracheal decellularized matrix was prepared by taking the fresh tracheal tissue of the isolated pig, removing the epithelial tissue and submucosa cell components, removing 70% of the chondrocyte components, retaining the tracheal extracellular matrix, and making a porous three-dimensional network structure:

[0043] (1) The fresh tracheal tissue of pigs obtained by routine processing, the attached fat tissue was cut off, and placed in phosphate-buffered saline (phosphate-buffered saline, PBS) for transportation and preservation;

[0044] (2) Put the acquired tracheal tissue into hypotonic tris buffer solution (hypotonic tris buffer, Ph8.0) containing protease inhibitor phenylmethyl-sulfonyl fluoride (0.35ml / L) ) in a shaker at 4°C for 24 hours;

[0045] (3) 1% mild detergent triton was added to the above solution, and the treatment was continued for 24 hours. Hank's simulated body fluid rinse thoroughly;

[0046] (4) Add DNase and treat with RANase at 37°C...

Embodiment 3

[0052] Embodiment 3 animal experiments

[0053] The decellularized trachea substitute prepared in the above example was inoculated with ciliated columnar epithelial cells from patients with tracheal tumors, cultured in an in vitro bioreactor, and then implanted into an experimental animal pig to replace the left common bronchus with a length of 4 cm. The results It shows that the main components of this trachea substitute are collagen and cartilage matrix, which can be used as a natural matrix for tissue regeneration and has good biocompatibility with tissues, without antigen interference, and can attract and gather human cells as a The base reproduces and regenerates, can quickly revascularize and re-epithelialize, and has good air tightness, ductility and elasticity; because its outer surface is reinforced with a spiral polyester material, its strength is strengthened to prevent collapse during inhalation, Its helical design minimizes the amount of polyester material used wi...

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Abstract

The invention belongs to the biomedical engineering field, and relates to a trachea substitute and a preparation method therefor. The trachea substitute has a porous and three-dimensional network structure, is a tubular, tegular or Y-type decellularized biomaterial, and comprises biological materials of allogeneic or heterogeneous sources. There are holes on the outer surface and the outer surface is seamed with spiral polyester materials. Experiments show that the trachea substitute has good biocompatibility with tissues, has no antigen interference effects, can perform revascularization and re-epithelization rapidly, has good air impermeability, ductility and elasticity, and can prevent collapse during breath. The trachea substitute can be made into different shapes and specifications, is suitable for tracheal reconstructive operations of tubular trachea substitution, juga molding, tracheal patching and the like. The trachea substitute is especially suitable for post-operation trachea substitution of trachea tumours, trachea trauma, benign tracheal stenosis, tumour invasion, congenital trachea malformation and the like.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, in particular to a trachea substitute and a preparation method thereof, in particular to a decellularized trachea substitute and a preparation method thereof. Background technique [0002] Tracheal surgery has a history of more than 100 years, and certain tracheal diseases involved in it often require appropriate tracheal substitutes after surgery, such as tracheal tumors, tracheal trauma, benign tracheal stenosis, and other tumors (lung cancer, esophageal cancer, thyroid cancer, etc.) For tracheal replacement after thoracic surgery such as tracheal violation and congenital tracheal deformity, so far, there is no tracheal substitute widely accepted by clinical tracheal surgery practice. Tracheal substitutes can be roughly divided into two categories: allogeneic trachea and artificial synthetic materials. Among them, revascularization and re-epithelialization are technical problems that trach...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/18A61F2/04
Inventor 谭强潘银根
Owner SHANGHAI CHEST HOSPITAL
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