77results about How to "Ability to inhibit metastasis" patented technology

The invention discloses an anti-tumor nano medicine based on cross-linking biodegradable polymer vesicae and a preparation method of the anti-tumor nano medicine. The anti-tumor nano medicine is prepared by loading a medicine on reversible cross-linking biodegradable polymer vesicae of asymmetric membrane structures; the medicine is a protein medicine, a polypeptide drug or a small molecule medicine; the reversible cross-linking biodegradable polymer vesicae of the asymmetric membrane structures are prepared from a polymer in a manner of self-assembling and cross-linking in sequence; the molecular chain of the polymer comprises a hydrophilic chain segment, a hydrophobic chain segment and PEI molecules which are connected in sequence; the hydrophobic chain segment comprises a polycarbonate chain segment and / or polyester chain segment; the medicine can be compounded and loaded under the electrostatic interaction of PEI; a membrane is reversible cross-linking biodegradable polyester / polycarbonate which is good in compatibility; dithiolane of a side chain is similar to a human body natural antioxidant lipoic acid; a shell takes PEG as a background and can target to cancer cells; the anti-tumor nano medicine has the potential to be a nano medicine system with advantages of simplicity, stability, multifunction and the like.

The invention discloses a polyphenol extract of trembling poplar as well as an extraction method and application thereof. The extraction method of the polyphenol extract of trembling poplar comprises the following steps: baking a trembling poplar plant material at 35 DEG C, and crushing; extracting with 95% ethanol, distilling to remove a solvent to obtain residues; dissolving the residues with chloroform, filtering to obtain filtrate and a primary residue, and distilling the filtrate in reduced pressure to obtain the polyphenol extract; and dissolving the primary residue by use of methanol, and distilling the solvent at reduced pressure to obtain the polyphenol extract. The polyphenol extract of the trembling poplar is convenient to extract, has high content, can be applied to industriallarge-scale production, has remarkable effects in removing oxygen radical of animals, inhibiting generation, proliferation and transfer of tumor cells of animals, is practical in treating cancer cells, and can generate good economical and social benefits.

The invention relates to platinum drugphotosensitizer-loaded protein nanoparticles and a preparation method and application thereof. The platinum drugphotosensitizer-loaded protein nanoparticles comprise platinum drugphotosensitizer complexes and proteins wrapping the platinum drugphotosensitizer complexes. Chemical therapy and photodynamic therapy are combined through an albumin nano-drug delivery system so that a synergistic anti-cancer effect is achieved, and the toxicity-reducing and efficacy-improving treatment effect is achieved. The nanoparticles prepared by the method are small in particle size, uniform in dispersion and round in shape; good chemical stability and illumination stability are realized; under the irradiation of near-infrared light, the nanoparticles have relatively high active oxygen generation capability; in addition, in cell experiments and animal experiments, it is verified that the nanoparticle has strong cytotoxicity and good in-vivo tumor targeting to tumorcells, plays a synergistic effect, reduces toxic and side effects, realizes combined treatment of tumors by chemotherapy and photodynamic therapy, and inhibits tumor metastasis.

The invention relates to an in-vitro culture device for highly simulating cancer cell migration. The in-vitro culture device comprises a first culture container body, a second culture container body and an osmosis supporting film and is characterized in that the second culture container body is clamped to the first culture container body through an annular retainer block arranged at the top of thesecond culture container body, part of the second culture container body extends into the first culture container body, and the osmosis supporting film is arranged at the bottom end of the second culture container body. The in-vitro culture device has the advantages that the in-vitro culture device is simple in structure and high in practicality, tumor cell nutrition supply, interstitial cells, blood vessels and vascular endothelial cells are considered, the device is close to the growth and transfer environment of in-vivo tumor, tumor cells are transferred into the first culture container body through the vascular endothelial cell so as to simulate the tumor cells entering blood vessels through capillary wall cell layers, and cell migration ability is judged by calculating the number ofthe tumor cells in the first culture container body after culture is performed for a certain period of time.

The invention discloses application of EB virus encoded microRNA BART6-3p in preparation of a nasopharyngeal carcinoma cell inhibitor. The microRNA BART6-3p is used for preparing a preparation for inhibiting growth and reproduction of nasopharyngeal carcinoma cells and preventing tumor cell invasion and metastasis. The microRNA BART6-3p has a sequence of CGGGGAUCGGACUAGCCUUAGA. The microRNA BART6-3p is further used for preparing a preparation for assembling and reconstructing a nasopharyngeal carcinoma cell inhibition framework. The microRNA BART6-3p provides a powerful molecular biological tool to assistant treatment of nasopharyngeal carcinoma, and has far-reaching clinical magnificence and important popularization application prospect.

The invention relates to a preparation method of a micro RNA nano microsphere and an application of the micro RNA nano microsphere in anti-tumor aspects. The nano microsphere comprises a nano-carrier and an antisense strand miR-125b-AS of micro RNA miR-125b which is wrapped or adsorbed by the carrier and is synthesized in vitro. According to the preparation method, miR-125b-AS is prepared into the nano microsphere, the stability of the nano microsphere can be significantly increased, and the nano microsphere cannot be degraded after being stored for a week at 25 DEG C; and detected by an MTT process, the nano microsphere can be transfected into a human neuroglioma cell U87 cell without using a transfection reagent, and the proliferation of tumor cells can be significantly inhibited. Invasion and migration experiments show that the transfer ability of the tumor cells can be significantly inhibited by the nano microsphere; influences of the nano microsphere to tumor cell cycles and cell apoptosis can be detected by using flow cytometry and a TUNEL process; and meanwhile, RT-PCR and Western-blot detection results show that changes of corresponding target genes and protein expression can be caused by the nano microsphere.

The invention discloses an application of rabeprazole sodium in preparation of medicines for inhibiting tumor cell metastasis and spread. The metastasis capability of tumor cells is inhibited by utilizing the medicines prepared from the rabeprazole sodium, the rabeprazole sodium refers to a low-toxicity medicine, a great blocking effect can be achieved in the tumor spreading process, meanwhile, treatment is performed by adopting other related tumor drugs, and the death amount of normal cells can be greatly reduced; and moreover, most of the existing tumor drugs are cytotoxic drugs, and lots of normal cells are killed when the tumor cells are killed.

The present invention provides novel IgE antibodies useful for inhibiting or preventing metastatic cancer. Also provided are methods to inhibit tumor metastasis by modulating the activity of at least one non-tumor cell, treating a patient to inhibit or prevent the appearance of tumor metastases derived from a primary solid tumor, treating established metastatic carcinoma, reducing metastasis of carcinoma cells, and reducing the growth kinetics of a primary solid tumor or a metastasized cell or tumor.

The present invention provides novel IgE antibodies useful for inhibiting or preventing metastatic cancer. Also provided are methods of inhibiting tumor metastasis by modulating the activity of at least one non-neoplastic cell, treating a patient to inhibit or prevent the appearance of tumor metastases derived from a primary solid tumor, treating established metastases, reducing cancer cell Metastasis, as well as slowing the growth kinetics of primary solid tumors or metastatic cells or metastases.

The invention belongs to the field of biomedical research, and in particular relates to the use of human EDDM3A gene as a target in the preparation of drugs for treating gastric cancer. After extensive and in-depth research, the present invention finds that down-regulating the expression of human EDDM3A gene by RNAi method can effectively inhibit the proliferation of gastric cancer cells, promote cell apoptosis, and effectively control the growth process of gastric cancer. The siRNA provided by the present invention or the nucleic acid construct containing the siRNA sequence, lentivirus can specifically inhibit the proliferation rate of gastric cancer cells, promote the apoptosis of gastric cancer cells, inhibit the cloning of gastric cancer cells, inhibit the metastasis ability of gastric cancer cells, and inhibit the growth of gastric cancer, thereby treating Gastric cancer, opening up a new direction for the treatment of gastric cancer.

The invention discloses a reversible cross-linked biodegradable polymer vesicle with an asymmetric membrane structure, a preparation method thereof and an application in nucleic acid medicine. Synthesized triblock polymer PEG-P(TMC-DTC)-PEI, PEG-P(DLLA-DTC)-PEI self-assembled and self-crosslinked to obtain polymersomes with asymmetric membrane structure, or connected with Targeted RCCPs are self-assembled by targeting molecules, the inner shell is PEI for complexing and loading nucleic acids through electrostatic interaction; the membrane is reversibly cross-linked biodegradable and biocompatible polyester / polycarbonate, side chain The dithiolane is similar to the human body's natural antioxidant lipoic acid, and the outer shell is based on PEG, which can target cancer cells. To study carrier complex functional siRNA, its in vivo and in vitro gene silencing effects, in vivo blood circulation and biodistribution, treatment of mice bearing orthotopic lung cancer and toxic and side effects, it is expected to become a simple, stable, multifunctional nano System platform for efficient, active targeted delivery of siRNA to tumors in situ.

The invention discloses application of siRNA of long non-coding RNA LINC00152 and an inhibiting preparation. The siRNA sequence of the target LINC00152 is designed and synthesized, the siRNA is transfected into an oral cancer cell line, and thus it is proven that the expression of the target interfering LINC00152 can obviously inhibit the invasion and transfer ability of oral cancer cells. A new potential approach is provided for treatment of oral cancers.