Application of rabeprazole sodium in preparation of medicines for inhibiting tumor cell metastasis and spread
A technology of rabeprazole sodium and tumor cells, applied in the pharmaceutical field of rabeprazole sodium, can solve problems such as the pharmacological effect of rabeprazole sodium has not been found, and achieve the effect of reducing the number of deaths and shortening the research and development cycle.
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Embodiment 1
[0024] Example 1: Application of Rabeprazole Sodium in the Preparation of Drugs for Inhibiting Metastasis and Spread of Colon Cancer Cells. The specific experimental plan is as follows:
[0025] 1. Experimental materials
[0026] Colon cancer cells (HT29) were used in the experimental stage of the present invention, and the drug used in the positive control was nocodazole (experimental concentration: 10uM). The negative control group received no treatment.
[0027] Wherein experiment adopts rabeprazole sodium compound C 18 h 20 N 3 NaO 3 S, CAS NO. is 117976-90-6, the concentrations are 100uM, 80uM, 60uM, 50uM, 40uM.
[0028] 2. Experimental method
[0029] For colon cancer cells cultured for 24 hours (cell density: 10 5 ) for drug stimulation for 2 hours:
[0030] (1) Centrifuge the colon cancer cells in a better state in the culture dish, and add an appropriate amount of culture medium to make a cell suspension;
[0031] (2) Get the cell suspension count, according...
Embodiment 2
[0039] Example 2: Transwell cell migration assay was used to determine the effect of rabeprazole sodium on the migration ability of colon cancer cells.
[0040] Will 1×10 5 The HT29 cells were inoculated in Transwell chambers with a 12.0 μm membrane at the bottom, and continued to be cultured. After the cells adhere to the wall, add DMEM complete medium with different concentrations of rabeprazole sodium to the upper chamber. To maintain osmotic pressure, add 0.05%-0.2% BSA, and add DMEM complete medium containing 15% FBS to the lower chamber and continue to cultivate 24h. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 2% PFA at 37°C for 10 minutes and then stained with Giemsa. Ten visual fields were randomly selected under a high-power microscope to count the migrated cells.
[0041] Cell migration rate = [the average number of cells in t...
Embodiment 3
[0045] Example 3: The damage repair experiment detects the effect of rabeprazole sodium on the migration ability of tumor cells.
[0046] Take HT29 cells in the logarithmic growth phase, and add 1×10 cells to each well of a 24-well plate 5 After the cells grow into a single layer, draw a "one"-shaped scratch, wash with PBS, add different concentrations of rabeprazole sodium in the control group and the experimental group, and take pictures. After 22 hours of culture, the same part is taken again to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / (0h average value of the blank control group-22h average value of the blank control group)]×100%.
[0047]Rabeprazole sodium acts on LTEP and A549 cells for 22 hours at different concentrations (40-100μg / ml) to inhibit migration. The results show that rabeprazole sodium has a dose-dependent inhibito...
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