In-vitro culture device for highly simulating cancer cell migration

Abstract

The invention relates to an in-vitro culture device for highly simulating cancer cell migration. The in-vitro culture device comprises a first culture container body, a second culture container body and an osmosis supporting film and is characterized in that the second culture container body is clamped to the first culture container body through an annular retainer block arranged at the top of thesecond culture container body, part of the second culture container body extends into the first culture container body, and the osmosis supporting film is arranged at the bottom end of the second culture container body. The in-vitro culture device has the advantages that the in-vitro culture device is simple in structure and high in practicality, tumor cell nutrition supply, interstitial cells, blood vessels and vascular endothelial cells are considered, the device is close to the growth and transfer environment of in-vivo tumor, tumor cells are transferred into the first culture container body through the vascular endothelial cell so as to simulate the tumor cells entering blood vessels through capillary wall cell layers, and cell migration ability is judged by calculating the number ofthe tumor cells in the first culture container body after culture is performed for a certain period of time.

Description

technical field [0001] The invention relates to the technical field of tumor cell detection, in particular to an in vitro culture device for highly simulating tumor cell metastasis. Background technique [0002] Tumor tissue in the human body is a cell population composed of tumor cells, stromal cells (including fibroblasts, inflammatory and immune cells), blood vessels, and extracellular matrix. The microenvironment in which tumor cells grow has an important impact on tumor growth. The traditional test methods for detecting the metastatic ability of tumor cells include scratch test and transwell test. Among them, the scratch test is to inoculate a single layer of adherent cells in a culture dish, draw a line in the central area of ​​cell growth to remove some cells, and observe the migration area of ​​the cells at a specified time to judge the migration ability of the cells. In the Transwell experiment, tumor cells are inoculated in the upper chamber, and FBS or some speci...

AI Technical Summary

Problems solved by technology

These two experimental methods suspend tumor cells in the culture medium or attach them to the wall of the culture dish, and do not form an environment similar to that of tumors in vivo, which greatly reduces the accuracy of detecting the migration ability of tumor cells

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