Polyphenol extract of trembling poplar as well as extraction method and application thereof
A technology of tulip tree and polyphenols, which is applied to the polyphenol extract of tulip tree and its extraction field, can solve problems such as unresearched medicinal value of hybrid trees, achieve good economic and social benefits, inhibit the The production and preparation process is simple
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Embodiment 1
[0021] Take freshly collected tulip tree bark and dry it in air at 35°C. Grind the dry material to medium fine powder, about 40 mesh, and set aside.
[0022] Take 350g of fine powder, then extract with 95% ethanol, and extract 3 times with 500ml; filter, combine the filtrates, and evaporate to remove the solvent to obtain a residue of about 35.0g; dissolve the residue with chloroform, dissolve 3 times in 250mL, distill off the chloroform solvent to obtain 8.4g of the product LTB2 and the first residue; the first residue was dissolved with methanol, 250mL was dissolved three times, and the solvent was distilled under reduced pressure to obtain 13.3g of the product LTB3. Put each product into a sterile screw cap bottle and store at -20°C for later use. For bioassays, each extract was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mg / mL and stored at -20°C.
[0023] The method of extracting dried leaves and bark of other Liriodendron species is the same as abov...
Embodiment 2
[0032] Take freshly collected tulip tree bark and dry it in air at 35°C. Grind the dry material to medium fine powder, about 40 mesh, and set aside. Get fine powder 350g, then extract with 90% ethanol, 500ml extract 3 times; Filter, combine filtrate, evaporate to remove solvent, obtain residue; Dissolve residue with chloroform, dissolve 3 times in 250mL, distill and remove chloroform solvent, obtain product LTB2 and The first residue: dissolve the first residue with methanol, dissolve 250 mL three times, and distill the solvent under reduced pressure to obtain the product LTB3. Put each product into a sterile screw cap bottle and store at -20°C for later use. The method of extracting dried leaves and bark of other Liriodendron species is the same as above.
[0033] Table 3 shows the extract yields of different plant tissues.
[0034] Table 3 Extract yield table of different plant materials
[0035]
[0036] In the table, LT: Liriodendron tulipifera; LC: Chinese mandarin...
Embodiment 3
[0041] MCF-7 cells were cultured using ATCC complete medium, including basic medium EMEM (Eagle's Minimum Essential Medium), balanced salt solution EBSS (Earles Balanced Salt Solution), non-essential amino acids, 2mM L-glutamine, 1 mM sodium pyruvate , 1.5mg / mL sodium bicarbonate solution, 0.01mg / mL bovine insulin, 10% fetal bovine serum, 25cm containing 100U / mL penicillin and 100 mg / mL streptomycin 2 Tissue culture flasks. Temperature controlled at 37°C, in the presence of 5% (v / v) CO 2 incubated in saturated water vapor.
[0042] Breast cancer MDA-MB-231 cells were cultured with ATCC complete medium, including L-15 and 2 mM L-glutamine, 1.5 mg / mL sodium bicarbonate, 10% fetal bovine serum, containing 100 U / mL penicillin and 100 mg / mL 25 cm mL of streptomycin 2 Tissue culture flasks, temperature controlled at 37°C, in 5% (v / v) CO 2 incubated in saturated water vapor.
[0043]The culture conditions of the other three human tumor cells were the same as the above two, excep...
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