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Application of etomoxir to treatment of colorectal cancer

A technology of ethyl moxher and colorectal cancer, applied in the field of medicine, can solve the problems of recurrence and metastasis, and the effect of chemotherapy needs to be improved, and achieve the effect of inhibiting metastasis, remarkable therapeutic effect and remarkable effect.

Active Publication Date: 2018-03-02
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of chemotherapy still needs to be improved, and a large part of the reason is also attributed to the recurrence and metastasis caused by drug resistance.

Method used

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  • Application of etomoxir to treatment of colorectal cancer
  • Application of etomoxir to treatment of colorectal cancer
  • Application of etomoxir to treatment of colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Effect of Etomoxir on Soft Agar Colony Formation of Colorectal Cancer Cells

[0028] 1. Experimental materials

[0029] (1) Drugs: Etomoxir.

[0030] (2) Colorectal cancer cells: human colon cancer cells HCT 15 and human colon adenocarcinoma cells HCT116.

[0031] (3) Commercially available soft agar.

[0032] 2. Experimental grouping

[0033] (1) Control group: blank control, that is, the cancer cells were not treated with any drugs.

[0034] (2) Experimental group: Etomoxir was used to treat cancer cells.

[0035] 3. Soft agar colony formation assay to detect the effect of etomoxir on the colony formation of colorectal cancer cells

[0036] (1) Lay soft agar in a six-well plate, then add colorectal cancer cells, and after solidification, add DMSO (ie control) and 50 μM, 100 μM etomoxer to treat the cells for 3 weeks, and then observe the cells through a microscope clone formation.

[0037] The specific method is as follows:

[0038] 1) After high-tem...

Embodiment 2

[0044] Example 2 Effect of Etomoxir on Anoikis of Colorectal Cancer Cells

[0045] 1. Experimental materials

[0046] (1) Drugs: Same as Example 1.

[0047] (2) Cancer cells: same as in Example 1.

[0048] (3) Commercially available apoptosis kits.

[0049] 2. Detection of anoikis of HCT15 and HCT116 cells after etomoxir treatment by flow cytometry

[0050] (1) Lay out colorectal cancer cells in a low-adhesion 6-well plate, add DMSO (ie control) and 50 μM, 100 μM emoxicillin to treat the cells, and then use the cell apoptosis kit to detect after 72 hours The case of anoikis.

[0051] The specific method is as follows:

[0052] 1) Digest the HCT15 and HCT116 cells in the logarithmic growth phase with trypsin, and take 1×10 6 Cells were plated in low-adhesion 6-well plates.

[0053] 2) Add the medium containing DMSO (ie control) and 50 μM, 100 μM etomoxir to treat the cells.

[0054] 3) After 72 hours, the cells were collected, and after trypsinization, the cells were pr...

Embodiment 3

[0057] Example 3 Effects of Etomoxir on Metastasis of Colorectal Cancer Cells and Body Weight of Nude Mice

[0058] 1. Experimental materials

[0059] (1) Drugs: Same as Example 1.

[0060] (2) Colorectal cells: Same as Example 1.

[0061] (3) Commercially purchased nude mice.

[0062] 2. The effect of etomoxir treatment on the metastasis of HCT15 and HCT116 cells in vivo was detected by tail vein injection nude mouse model

[0063] (1) HCT15 and HCT116 cells were injected into the tail vein to construct a nude mouse metastasis model of colorectal cancer, and the cells were treated with 40 mg / kg of etomoxir to detect the effect of etomoxir on the metastasis of colorectal cancer cells in vivo.

[0064] The specific method is as follows:

[0065] 1) Digest the HCT15 and HCT 116 cells in the logarithmic growth phase with trypsin, and take 2×10 6 Cells were resuspended in 100 microliters of PBS.

[0066] 2) Put 2×10 6 HCT15 and HCT116 cells were injected into the tail vein of...

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Abstract

The invention discloses application of etomoxir to treatment of colorectal cancer and particularly relates to application of etomoxir to preparation of medicines for preventing and treating colorectalcancer, especially medicines for inhibiting colorectal cancer metastasis. The research shows that etomoxir is capable of obviously inhibiting colony formation under anoikis of colorectal cancer cells, accelerating anoikis of colorectal cancer cells and inhibiting metastasis capability of the colorectal cancer cells in the body, has an obvious effect of treating the colorectal cancer and especially has an obvious effect of inhibiting colorectal cancer metastasis, so that etomoxir can be used for treating colorectal cancer metastasi. The invention provides novel application of etomoxir, expandsthe clinical application fields of etomoxir and further provides a novel manner for treating colorectal cancer metastas; etomoxir has good popularization and application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to the application of etamoxir in the treatment of colorectal cancer. Background technique [0002] Colorectal cancer is a common gastrointestinal tumor in the world, ranking first among all kinds of malignant tumors in my country, and one of the main causes of death worldwide, and there are great difficulties in clinical treatment. [0003] The incidence and mortality of colon cancer are high, and doctors in this field are also troubled by the problem of tumor drug resistance in chemotherapy. According to the estimates of the American Cancer Society, more than 90% of tumor patients died of different degrees of drug resistance. The problem of tumor drug resistance has become a key factor for the success of cancer chemotherapy. Oxaliplatin, the first-line chemotherapy drug recommended by the guidelines, is one of the most commonly used chemotherapy drugs. However,...

Claims

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Application Information

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IPC IPC(8): A61K31/336A61P35/00
CPCA61K31/336
Inventor 徐瑞华王英男鞠怀强
Owner SUN YAT SEN UNIV CANCER CENT
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