34 results about "Cytimidine" patented technology

The invention relates to methods for preparing 5-Aza-2'-deoxycytidine and an intermediate product thereof. The method for preparing the preparing 5-Aza-2'-deoxycytidine comprises the following steps: a compound (shown in formula 2) is hydrolyzed under alkaline matter to remove acyl-oxygen protecting group, a diastereomeric compound (shown in formula 3) is obtained, and then the diastereomeric compound is separated by a general separation technology to obtain an isomer. The invention also relates to a compound (shown in formula 2) used as the intermediate product and a preparation method thereof, wherein the preparation method comprises the following steps: 5-Aza cytimidine is activated by a silanization method, and then makes displacement reaction with a compound (shown in formula 4), and further removes the protecting group to obtain the compound (shown in formula 2). The preparation method has simpler process, good reproduction quality and little toxicity and is suitable to the industrialized production; and meanwhile, used raw materials and reagent are easy to obtain.

The invention discloses a method for synthesizing cytimidine, which comprises the following steps: performing a reaction of a reactant (1) and a reactant (2) to acquire cytimidine under the action of sodium methoxide, wherein the reactant (1) is 3-alkoxy acrylonitrile and/or 3, 3-alkoxy propionitrile, and the reactant (2) is carbamide. The method is characterized in that a solid catalyst is added in the reaction. The catalyst is the solid catalyst, such as one or more of acidic alumina, solid super acid, zinc oxide and a molecular sieve. The method can achieve the effects of shortening the reaction time, improving the yield and reducing the price of raw materials.

The invention relates to the medical biology detection technical field and discloses a method for quickly detecting DNA methylation. Aiming at the disadvantages of the prior method, the method provided by the invention improves the following three aspects: 1. catalyst TAC and guanidine hydrochloride are added to accelerate the modification reaction; 2. short-time high-temperature melting is performed during the modification reaction for times so as to fully expose basic groups to transform cytimidine which is not made from methylation modification to sulfonated uracil; 3. aiming at fussy dialysis desalination steps after the modification, when a modified DNA product is purified, the modified DNA product is combined into a glass fiber membrane or pellosil or other pillars for washing desalination, then is treated by sulfated reagent and eluted, thereby obtaining a methylated PCR template which can be directly used for PCR augmentation and greatly saves desalination time. The method has low cost, short detection time and good repetitiveness.

The invention discloses a method for predicting malignant tumor metastasis and invasion capacity in vitro, which comprises the following steps of: (a) extracting DNA (Deoxyribonucleic Acid) from surgical incisal margins or tumor-adjacent tissues; (b) detecting the methylating degree of cytimidine in 5'- end CpG island of seroreaction factor gene DNA sequence in DNA obtained in the step (a); and (c) when the seroreaction factor gene DNA sequence with methylated cytimidine in the 5'-end CpG island is detected in the step (b), presuming that the tissue being detected has the capacity of resisting malignant tumor metastasis and invasion. The invention also provides four artificial nucleotide fragments. The inventor of the invention, for the first time, find that the metastasis and invasion capacity of malignant tumors can be rapidly and accurately predicted by detecting whether the seroreaction factor gene DNA sequence with methylated cytimidine in CpG island exists or not in the tissue being detected. Besides, the nucleotide fragments provided in the invention can be used for accurately and rapidly detecting the methylation of cytimidine in the CpG island in the seroreaction factor gene DNA sequence.

The invention relates to a gamma-cytidine-5'-disodium triphosphate crystal, and a preparation method, a drug composition, and a drug preparation of the crystal. The solubility and the stability of the crystal are better than those of a substance in the prior art, and the crystal is more beneficial for pharmacy and long-term storage. The preparation is particularly suitable for being prepared into an injection and a powder injection.

The invention discloses a novel synthesis process of capecitabine and discloses a preparation method of 2',3'-O-isopropylidene-5'-deoxy-5-fluoro-N4-(carbopentyloxy)cytimidine (compound V) serving as an intermediate. Meanwhile, the invention discloses a preparation method for obtaining capecitabine by removing a protective group from the compound serving as a raw material under the catalytic action of iodine. The method has the advantages of readily available raw materials, environmental friendliness, high yield and no heavy metal pollution, and is suitable for industrial production.

The invention discloses a method for synthesizing cytidine, and belongs to the field of nucleoside synthesis in organic chemistry. Cyanoacetaldehyde urea is used as a raw material, is subjected to silylation and then is condensed with tetraacetylribose under the catalytic action of Lewis acid to obtain an intermediate, and then cyclization and deprotection are completed through a one-pot reactionunder the action of sodium alcoholate to obtain cytidine. The method is convenient in raw material source and simple to operate, the three steps of reaction processes are continuously carried out, andrecrystallization purification only needs to be carried out when a final product is obtained; compared with the traditional process, the process operation is simple, the yield is stable, and the final alkaline hydrolysis and condensation processes are carried out by a one-pot method, thereby facilitating industrial large-scale production.

The invention discloses a 2',3'-O-carbonyl-5'-deoxy-5-fluoro-N4-[(pentyloxy)carbonyl]cytidine synthesis method. According to the method, 2,3-di-O-acetyl-5'-deoxy-5-fluoro-N4-[(pentyloxy)carbonyl]cytidine is adopted as a raw material, and is dissolved by using an organic solvent; organic alkali is added; CO2 is delivered in; and a reaction is allowed under a reaction temperature controlled at 0-50 DEG C and a reaction time controlled at 1-24h. According to the method, an intermediate of a capecitabine production process is used for preparing capecitabine impurity C2',3'-O-carbonyl-5'-deoxy-5-fluoro-N4-[(pentyloxy)carbonyl]cytidine. The raw materials are easier to obtain, and the method is more simplified.

The invention discloses a synthesis process of uridine, and belongs to the technical field of organic synthesis. The process comprises the following steps of uniformly mixing cytidine serving as a main raw material with water, controlling the temperature to be 10-60 DEG C, respectively adding a nitrous acid reagent and inorganic acid or organic acid, carrying out heat preservation reaction for 1-10 hours, ending the reaction after the raw material is added, controlling the pH value to be 1-4 after the reaction is completed, carrying out reduced pressure concentration to obtain a uridine concentrated solution, dropwise adding an alcohol solvent into the uridine concentrated solution, and crystallizing to obtain the product uridine. The process is easy to operate, thorough in reaction and high in reaction speed, and the synthesis period is greatly shortened. In addition, the conversion rate of the process is up to 90% or above, the yield is up to 80% or above, and the purity is greater than 99.5%.

The invention belongs to the technical field of metabolic markers, and relates to application of cytidine serving as a metabolic marker for predicting death caused by acute and severe radioactive intestinal injuries. With the application of the cytidine serving as the metabolic marker for predicting death caused by acute and severe radioactive intestinal injuries of the invention, death caused byacute and severe radioactive intestinal injuries can be better predicted.