34 results about "Toxin resistance" patented technology

The present invention provides improved formulations of botulinum toxin that increase delivery of the botulinum toxin to neural and associated tissues and exhibit a higher specific neurotoxicity and higher potency (in LD50 Units) than available formulations of botulinum toxins. These improved formulations enable physicians to treat a wide variety of pathological conditions with a lower toxin load that reduces the risk of inducing an immune response against the toxin and its associated proteins that may ultimately lead to the development of toxin resistance. These benefits are particularly important in the treatment of conditions that require high-dose or chronic administration of botulinum toxin. Additionally, the decreased in LD50 Unit doses of inventive formulations allows for controlled administration limits diffusion. The present invention also provides methods of treating neuromuscular diseases and pain, using low-dose botulinum toxin.

The invention discloses a brewing yeast strain, which has the classified name Saccharomyces cerevisiae Y09tj, and is preserved in Common Microorganism Center of China General Microbiological Culture Collection Center with the preservation number of CGMCC No.3476. The invention also provides a breeding method for the strain and a method for producing ethanol by quick high-temperature thick mash fermentation of starch. The strain is obtained by the following steps of: separating from common self-brewed sweet wine of peasant families; and performing ultraviolet mutagenesis for breeding. The brewing yeast strain CGMCC NO.3476 has the advantages of quick growth, strong fermentation capacity, high ethanol toxin resistance, and high-glucose concentration resistance of 62 percent (m / v) to the maximum. The average wine yield by the method is improved by 1.2 percent than that of other producing strains, and the wine yield can reach 16.0 percent (v / v); the fermentation period is short, and only 46 hours; the equipment utilization rate can be greatly improved; and the fermentation cost is greatly reduced.

The invention discloses a double-metal co-doped carbon nano-composite material and a preparation method thereof. The composite material is prepared from a carbon substrate and ferrocene-phenylalanine and transition metal except iron, which are co-assembled on the carbon substrate through a non-covalent bond, wherein the ferrocene-phenylalanine, the transition metal except the iron and the carbon substrate commonly form a raspberry-like nanosphere structure. The invention further discloses a double-metal-nitrogen-carbon nano-catalyst obtained by mixing the composite material with dicyanodiamide and carbonizing and further provides application of the double-metal-nitrogen-carbon nano-catalyst to catalytic oxygen reduction reaction. The preparation methods of the composite material and the catalyst have simple steps and low cost and are suitable for large-scale application. The double-metal-nitrogen-carbon nano-catalyst provided by the invention has excellent electrochemical performance and has good methanol toxin resistance and stability, so that the double-metal-nitrogen-carbon nano-catalyst has a good application prospect in the field of the catalytic oxygen reduction reaction.

The invention discloses a citrus laccase family gene CsiLAC4 and application thereof and belongs to the technical field of plant genetic engineering. A method for obtaining the citrus laccase family gene CsiLAC4 comprises the following steps: extracting total RNA (Ribonucleic Acid) from citrus leaves; (2) carrying out reverse transcription on the RNA obtained in the step (1) so as to obtain cDNA (Complementary Deoxyribonucleic Acid); (3) by taking the obtained cDNA as a template, carrying out a first round of PCR (Polymerase Chain Reaction) amplification by using a forward primer F1 and a reverse primer R1; (4) by taking an amplification product of the first round as a template, carrying out nest type PCR amplification by using the forward primer F1 and a reverse primer R2, thereby obtaining the citrus laccase family gene CsiLAC4. The citrus laccase family gene CsiLAC4 is adopted to improve the boron toxin resistance of plants, and provides new gene resources for abiotic stress resistant molecule breeding of plants.

In one aspect the present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaing a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml / min / 10 L of initial volume during induction along with a continuous inusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h. In another aspect, the present invention relates to a method of purifying a nonglycosylated immunotoxin comprising a) loading a solution containing the nonglycosylated immunotoxin onto a hydrophobic interaction column; b) obtaining a first non-glycosylated immunotoxin containing eluant from the hydrophobic interaction column; c) loading the non-glycosylated immunotoxin containing eluant from step (b) onto an anion exchange column; d) obtaining a second non-glycosylated immunotoxin containing eluant from the anion exchange column by eluting the non-glycosylated immunotoxin with a sodium borate solution; e) diluting the concentration of sodium borate in the second non-glycosylated immunotoxin containing eluant from step (d) to about 50 mM or less; f) concentrating the diluted non-glycosylated immunotoxin containing eluant from step (e) over an anion exchange column; and g) obtaining a purified non-glycosylated immunotoxin from the anion exchange column.

The present invention relates to the generation of stable haploid cell cultures, uses of said cells in forward and reverse genetics, especially the identification of target genes associated with a modified phenotype and in particular identifying genetic targets associated with toxin resistance, especially ricin toxicity resistance, and therapeutic uses of target compounds.

The invention provides skin-building toilet water rich in sodium copper chlorophyllin and a preparation method thereof. The skin-building toilet water uses sodium copper chlorophyllin as one of effective components and comprises main raw materials such as sodium copper chlorophyllin, wild chrysanthemum flower volatile oil, Artemisia leaf volatile oil, coconut oil acylamide propyl lycine, lemon extract, ethanol and water, and the steps comprise weighing, dissolution, emulsification, total mixing, uniform emulsification, filling, code spraying, packaging, and the like. The skin-building toilet water has simple preparation method and equipment and easy production and popularization. The skin-building toilet water rich in sodium copper chlorophyllin has the effects of mosquito expelling, prickly heat prevention, itching relieving, sterilization, detoxification, air freshening, toxin resistance, ulcer resistance, hemolysis resistance, white ascending, liver protection and yellow elimination, and can promote the concrescence of ulcer surfaces, prevent cold, cephalitis and skin pruritus, maintain the functions of the brain, the heart and the lung and improve the immunity. The skin-building toilet water rich in sodium copper chlorophyllin is especially suitable for infants and children.

The invention discloses a medicine for preventing and treating goldfish swim bladder disorder. The medicine contains the following raw materials: vegetable-and-fruit enzyme powder, herba plantaginis, rhizoma coptidis, radix astragali, cortex phellodendri, herba portulacae, tangerine peels, radix Aucklandiae, citrus aurantium, fructus aurantii, spikenard, sword beans, persimmon calyx, dried tangerine peels, fructus schizandrae, lycium barbarum, radix rehmanniae, dioscorea opposita, propolis, sulfamethoxazole and trimethoprim. The medicine can be used for remarkably improving the immune disease resistance of goldfish to swim bladder disorder and plays a role in treating the goldfish swim bladder disorder; the formula has the effects of aiding digestion, diminishing inflammation, resisting bacteria, benefiting intestines, and the like and has obvious effects of calming and lowering reversed flow of Qi, eliminating stagnation of Qi, treating enteritis, expelling toxins and improving toxin resistance of livers. The medicine for preventing and treating the goldfish swim bladder disorder and fish feed are combined and are made into granules of different grain sizes, and thus, the cure rate of the goldfish swim bladder disorder reaches 90% or more.

The invention discloses a nutrient solution for improving growth environment of aquatic plants. The nutrient solution is prepared from, by weight, 1-2 parts of nickel sulfate, 3-5 parts of ammonium chloride, 110-130 parts of rice bran, 10-14 parts of sodium silicate, 20-25 parts of radix isatidis, 18-22 parts of rhizoma coptidis, 35-40 parts of mung beans, 100-110 parts of raised maggots, 0.6-0.8 part of alpha-amylase, 0.5-0.7 part of trypsin, 4-7 parts of herba schizonepetae juice and an appropriate amount of water. The nutrient solution contains parts of Chinese herbal medicine ingredients, is easily absorbed through complexing with multiple types of amino acids, performs conductive metabolism through a variety of enzymes after entering plant bodies, can not only supplement protein, nitrogen, phosphorus, potassium and various trace elements for plants and also strengthen the toxin resistance of the plants, and the nutrient solution can also degrade harmful substances in soil and air through sedimentation and volatilization in the soil after being used and improve the growth environment of the plants.

The invention discloses a method for managing resistance of ostrinia nubilalis to Bt insecticidal protein. The method is realized by simultaneously expressing at least one Bt insecticidal crystal protein Cry and at least one Bt vegetative insecticidal protein Vip3 in the same plant cell. According to the invention, the coding gene of the Bt insecticidal crystal protein Cry and the coding gene of the Bt vegetative insecticidal protein Vip3 are simultaneously introduced into plant cells so that the obtained transgenic plant can simultaneously express two Bt toxins which have an efficient insecticidal effect on ostrinia nubilalis and do not have cross resistance, and the resistance of the ostrinia nubilalis to the Bt toxins can be effectively managed; the generation of Bt toxin resistance of corn borers can be greatly relieved, the service life of transgenic plants is greatly prolonged, important germplasm resource reserve is provided for achieving the national biological breeding strategy, and the method is creative improvement of an existing pest resistance control technology.

The invention aims to provide a pure traditional Chinese medicine additive for preventing reproductive comprehensive symptoms of sows. The pure traditional Chinese medicine additive for preventing reproductive comprehensive symptoms of sows is prepared from the following traditional Chinese medicines: angelica sinensis, teasel roots, bighead atractylodes rhizomes, caulis spatholobi, malt, poria cocos, red dates, Indian kalimeris herb, Chinese yams, rheum officinale and dandelions. The pure traditional Chinese medicine additive has effects of enriching qi and blood of sows, promoting development of reproductive accessories such as uterus and ovary, maintaining normal physiological functions, and protecting and nourishing fetus, improves disease resistance and toxin resistance of organisms,relieves toxicity of toxins to the reproductive accessories, well relieves a series of reproductive comprehensive disorders of sows such as non-oestrus, fake oestrus, infertility, fetus insufficiency,stillbirth, mummy fetus, long birth process, little piglets and weak piglets caused by deficiency of qi and blood, toxin accumulation, virus recessive infection, low reproductive organ function and the like in clinical use, can effectively improve a mating rate, a conception rate and a litter size of sows, and prevents the occurrence of reproductive disorder comprehensive syndromes of sows.

The invention discloses a bacterial blight-resistant japonica rice breeding method. The steps of the breeding method are as follows: (1) selecting a japonica rice variety / line to be improved and an indica rice resistance source resistant to bacterial blight; Improve japonica rice varieties / lines for crude toxin tolerance concentration identification; (3) cross breeding to obtain three-cross F1 hybrids; (4) anther culture for three-cross F1 hybrids; (5) field planting of flowers and seedlings , a number of japonica rice cultivar families with improved resistance to bacterial blight were harvested from a single plant, and different flower culture families were further planted in the field. Using the japonica rice variety / line parent to be improved as a control, the agronomically good shape and bacterial blight resistance were screened. A new strain of japonica rice with improved sex. The breeding method of the present invention can specifically and efficiently introduce the resistance to bacterial blight of indica rice into japonica rice, and the breeding for resistance to bacterial blight can be completed in about 3 years, which significantly shortens the conventional breeding cycle for resistance to bacterial blight, and has greater breeding efficiency. Value.

The disclosure relates to Bt toxin resistance management. One embodiment relates to the isolation and characterization of polynucleotides and polypeptides corresponding to novel Bt toxin receptors. The polynucleotides and polypeptides are useful in identifying or designing novel Bt toxin receptor ligands including novel insecticidal toxins.

The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.

The invention discloses a rice leaf roller cadherin Cry toxin binding region coding gene with a nucleotide sequence as shown in SEQ ID NO.14 and a coding protein with an amino acid sequence as shown in SEQ ID NO.15 for the first time. A toxin binding region CmCad-CR6-MPED of rice leaf roller cadherin has a binding effect with Cry1Ac toxin, can be applied to prediction of rice leaf roller Cry toxin resistance. If the nucleotide sequence of the cadherin Cry toxin binding region gene of rice leaf roller to be detected is different from SEQ ID NO.14, a rice leaf roller receptor is subjected to gene mutation, the binding capacity is reduced, and the insect has certain resistance to Cry toxin. The coding protein can also be used for screening Bt protein aiming at the rice leaf folder; and if the Bt protein to be screened and a code have high affinity, the Bt protein to be screened is inevitably inserted into the midgut BBMV of the rice leaf folder, epithelial cell membrane is broken, and the Bt toxin insecticidal protein with high toxicity to the rice leaf folder can be rapidly screened or modified.

The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.

The invention discloses a molecular detection method for resistance genes of spodoptera frugiperda to bacillus thuringiensis Bt Cry1F toxin. The method comprises the following steps: carrying out PCR amplification on a genome DNA template of the spodoptera frugiperda by utilizing a specific forward primer F as shown in SEQ ID NO.1 and a specific reverse primer R as shown in SEQ ID NO.2, and directly sequencing a PCR purified product; according to a sequencing chromatogram, identifying whether 12-bp base insertion occurs on a No.15 exon of the individual adenosine triphosphate (ATP) binding cassette transporter C2 gene of the spodoptera frugiperda; and distinguishing the sensitive homozygote, the resistant heterozygote and the resistant homozygote of the resistant gene at one time. The method has the advantages of rapidness, simplicity, convenience and high accuracy, and is specially used for molecular detection and early warning of the resistance of the spodoptera frugiperda to the Bt Cry1F toxin.

The invention provides a method for enhancing the toxin resistance of a rice bakanae disease sterilization agent. The method is characterized by sequentially comprising the following steps of 1, primary seed selection; 2, sprouting experiments; 3, seed insolation in sunny days: performing seed insolation for 1 to 2 days in sunny days; 4, seed selection by brine; 5, seed rinsing; 6, clean water gemmation promotion: soaking the selected seeds by clean water with the quantity capable of completely soaking the seeds, wherein the soaking time is 8 to 24 hours; 7, seed soaking by medicine: soaking the seeds subjected to gemmation promotion for sterilization for 24 to 48 hours by the sterilization agent; 8 fishing and drying: fishing and draining the sterilized seeds. A two-stage seed soaking method is used; the clean water gemmation promotion is used for realizing the resistance on the side effects of the sterilization agent; the promotion effect is achieved on the seed gemmation capabilityand seedling quality improvement; the economic properties and the practical yield are obviously improved through being compared with that of seed soaking by a single sterilization agent.

The invention discloses antrodia camphorate cigarettes. The antrodia camphorate cigarettes are prepared from tobacco shreds and an antrodia camphorate extracting solution, 3-4 parts by weight of the antrodia camphorate extracting solution is added into every 100 parts by weight of the tobacco shreds, and the antrodia camphorate extracting solution is added after the temperature of the tobacco shreds is increased before the tobacco shreds are dried. The invention further discloses a method for preparing the antrodia camphorate cigarettes. The antrodia camphorate cigarettes have the advantages of being high in biological activity and capable of enhancing the toxin resistance of a human body.

A method for monitoring resistance to a Bacillus thuringiensis pesticidal crystal protein is provided, which involves the measurement of protease activity or protease expression in the gut of adult insects.

The invention discloses a fireproof coating additive which is prepared from the following components in parts by weight: 125 parts of tourmaline superfine powder, 58 parts of propylene glycol monomethyl ether acetate, 29 parts of silicon dioxide, 169 parts of isopropyl alcohol, 79 parts of leveling agent, 98 parts of condensate oil, 66 parts of dimethyl carbonate, 34 parts of polysiloxane, 48 parts of oxidized carbon black and 132 parts of amide-polyamide mixture. Thus, the coating additive is reasonable in formula and can serve as an additive of a coating to improve the fireproof property, toxin resistance, insect prevention, insecticidal property and thermal insulation property of the coating; moreover, the coating additive is conductive to environmental protection.

The invention relates to a method for suppressing the Bt (Bacillus thuringiensis) CRY toxin resistance of insects by using toxins not needing cadherin receptors. The invention relates to a method for obtaining DNA constmcciones coding a Cry toxin (also known as Cry toxin, Bt toxin or delta-endotoxin) which has three structural domains lacking alpha-1 helixes. The DNA constmcciones is modified to code protein which can kill insects with resistance to a corresponding unmodified Cry toxin. The invention relates to DNA constmcciones of a modified 3-structural domain Cry toxin gene, modified 3-structural domain Cry protein, methods, a molecular carrier containing the DNA constmcciones, a host cell containing the DNA constmcciones and recombination methods for generating modified 3-structural domain Cry toxins. Besides, the invention relates to a preparation containing the modified 3-structural domain Cry toxins. The insect resistance to unmodified Cry toxins is caused by the reduction of the combination of the toxins and receptors in the intestines of the insects. More concretely, the invention relates to expression of modified 3-structural domain Cry toxins, methods for expressing the modified 3-structural domain Cry toxins, transgenic microbes and plants containing the DNA constmcciones for expressing the modified 3-structural domain Cry toxins, and a method for suppressing the unmodified CRY toxin resistance of insects.

The invention discloses a drug for preventing epidemic toxin infection and a production method thereof. The drug comprises the following raw materials: 5-7 parts of maca, 5-7 parts of agastache rugosa, 5-7 parts of honeysuckles, 5-7 parts of perilla leaves and 5-7 parts of dahurian angelica roots. The method sequentially and separately performs decoction, concentration, alcohol precipitation, filtration, spray-drying and granulation. The drug has good treatment and dehumidification effects. The maca is matched with the agastache rugosa, the honeysuckles, the perilla leaves and the dahurian angelica roots for the first time, so that the drug has dehumidification, cold prevention and epidemic toxin resistance effects. Multiple clinical practices prove that: the drug has good effect; and thecourse of treatment and the dosage of the drug are obviously shortened, and compared with traditional medicine formulas and production methods on the market, the cost of the medicine for preventing and treating epidemic virus infection prepared by the formula and the production method is obviously reduced.