34 results about "Toxin resistance" patented technology

The present invention provides improved formulations of botulinum toxin that increase delivery of the botulinum toxin to neural and associated tissues and exhibit a higher specific neurotoxicity and higher potency (in LD50 Units) than available formulations of botulinum toxins. These improved formulations enable physicians to treat a wide variety of pathological conditions with a lower toxin load that reduces the risk of inducing an immune response against the toxin and its associated proteins that may ultimately lead to the development of toxin resistance. These benefits are particularly important in the treatment of conditions that require high-dose or chronic administration of botulinum toxin. Additionally, the decreased in LD50 Unit doses of inventive formulations allows for controlled administration limits diffusion. The present invention also provides methods of treating neuromuscular diseases and pain, using low-dose botulinum toxin.

The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.

The invention discloses a citrus laccase family gene CsiLAC4 and application thereof and belongs to the technical field of plant genetic engineering. A method for obtaining the citrus laccase family gene CsiLAC4 comprises the following steps: extracting total RNA (Ribonucleic Acid) from citrus leaves; (2) carrying out reverse transcription on the RNA obtained in the step (1) so as to obtain cDNA (Complementary Deoxyribonucleic Acid); (3) by taking the obtained cDNA as a template, carrying out a first round of PCR (Polymerase Chain Reaction) amplification by using a forward primer F1 and a reverse primer R1; (4) by taking an amplification product of the first round as a template, carrying out nest type PCR amplification by using the forward primer F1 and a reverse primer R2, thereby obtaining the citrus laccase family gene CsiLAC4. The citrus laccase family gene CsiLAC4 is adopted to improve the boron toxin resistance of plants, and provides new gene resources for abiotic stress resistant molecule breeding of plants.

In one aspect the present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaing a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml / min / 10 L of initial volume during induction along with a continuous inusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h. In another aspect, the present invention relates to a method of purifying a nonglycosylated immunotoxin comprising a) loading a solution containing the nonglycosylated immunotoxin onto a hydrophobic interaction column; b) obtaining a first non-glycosylated immunotoxin containing eluant from the hydrophobic interaction column; c) loading the non-glycosylated immunotoxin containing eluant from step (b) onto an anion exchange column; d) obtaining a second non-glycosylated immunotoxin containing eluant from the anion exchange column by eluting the non-glycosylated immunotoxin with a sodium borate solution; e) diluting the concentration of sodium borate in the second non-glycosylated immunotoxin containing eluant from step (d) to about 50 mM or less; f) concentrating the diluted non-glycosylated immunotoxin containing eluant from step (e) over an anion exchange column; and g) obtaining a purified non-glycosylated immunotoxin from the anion exchange column.

The present invention relates to the generation of stable haploid cell cultures, uses of said cells in forward and reverse genetics, especially the identification of target genes associated with a modified phenotype and in particular identifying genetic targets associated with toxin resistance, especially ricin toxicity resistance, and therapeutic uses of target compounds.

The present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaining a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml / min / IO L of initial volume during induction along with a continuous infusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h.

The invention discloses a medicine for preventing and treating goldfish swim bladder disorder. The medicine contains the following raw materials: vegetable-and-fruit enzyme powder, herba plantaginis, rhizoma coptidis, radix astragali, cortex phellodendri, herba portulacae, tangerine peels, radix Aucklandiae, citrus aurantium, fructus aurantii, spikenard, sword beans, persimmon calyx, dried tangerine peels, fructus schizandrae, lycium barbarum, radix rehmanniae, dioscorea opposita, propolis, sulfamethoxazole and trimethoprim. The medicine can be used for remarkably improving the immune disease resistance of goldfish to swim bladder disorder and plays a role in treating the goldfish swim bladder disorder; the formula has the effects of aiding digestion, diminishing inflammation, resisting bacteria, benefiting intestines, and the like and has obvious effects of calming and lowering reversed flow of Qi, eliminating stagnation of Qi, treating enteritis, expelling toxins and improving toxin resistance of livers. The medicine for preventing and treating the goldfish swim bladder disorder and fish feed are combined and are made into granules of different grain sizes, and thus, the cure rate of the goldfish swim bladder disorder reaches 90% or more.

The invention discloses a nutrient solution for improving growth environment of aquatic plants. The nutrient solution is prepared from, by weight, 1-2 parts of nickel sulfate, 3-5 parts of ammonium chloride, 110-130 parts of rice bran, 10-14 parts of sodium silicate, 20-25 parts of radix isatidis, 18-22 parts of rhizoma coptidis, 35-40 parts of mung beans, 100-110 parts of raised maggots, 0.6-0.8 part of alpha-amylase, 0.5-0.7 part of trypsin, 4-7 parts of herba schizonepetae juice and an appropriate amount of water. The nutrient solution contains parts of Chinese herbal medicine ingredients, is easily absorbed through complexing with multiple types of amino acids, performs conductive metabolism through a variety of enzymes after entering plant bodies, can not only supplement protein, nitrogen, phosphorus, potassium and various trace elements for plants and also strengthen the toxin resistance of the plants, and the nutrient solution can also degrade harmful substances in soil and air through sedimentation and volatilization in the soil after being used and improve the growth environment of the plants.

The invention aims to provide a pure traditional Chinese medicine additive for preventing reproductive comprehensive symptoms of sows. The pure traditional Chinese medicine additive for preventing reproductive comprehensive symptoms of sows is prepared from the following traditional Chinese medicines: angelica sinensis, teasel roots, bighead atractylodes rhizomes, caulis spatholobi, malt, poria cocos, red dates, Indian kalimeris herb, Chinese yams, rheum officinale and dandelions. The pure traditional Chinese medicine additive has effects of enriching qi and blood of sows, promoting development of reproductive accessories such as uterus and ovary, maintaining normal physiological functions, and protecting and nourishing fetus, improves disease resistance and toxin resistance of organisms,relieves toxicity of toxins to the reproductive accessories, well relieves a series of reproductive comprehensive disorders of sows such as non-oestrus, fake oestrus, infertility, fetus insufficiency,stillbirth, mummy fetus, long birth process, little piglets and weak piglets caused by deficiency of qi and blood, toxin accumulation, virus recessive infection, low reproductive organ function and the like in clinical use, can effectively improve a mating rate, a conception rate and a litter size of sows, and prevents the occurrence of reproductive disorder comprehensive syndromes of sows.

The disclosure relates to Bt toxin resistance management. One embodiment relates to the isolation and characterization of polynucleotides and polypeptides corresponding to novel Bt toxin receptors. The polynucleotides and polypeptides are useful in identifying or designing novel Bt toxin receptor ligands including novel insecticidal toxins.

The disclosure relates to Bt toxin resistance management. One embodiment relates to the isolation and characterization of polynucleotides and polypeptides corresponding to novel Bt toxin receptors. The polynucleotides and polypeptides are useful in identifying or designing novel Bt toxin receptor ligands including novel insecticidal toxins.

The invention relates to the field of fuel cells, in particular to a novel DMFC (direct methanol fuel cell). The battery comprises a cell casing, a membrane electrode and an anode tube from outside to inside sequentially, wherein an electrolyte chamber is formed in the anode tube, an air chamber is formed between the casing and the membrane electrode, and a cathode output end and an anode output end are arranged at the cathode end and the anode end of the cell and connected with the cell casing through weld points respectively; the membrane electrode covers the surface of the anode tube; a feeding hole is formed in the electrolyte chamber of the casing, an air circulation hole is formed in the air chamber of the casing, a water discharge hole is formed in the bottom of the air chamber of the casing, and a CO2 discharge hole is formed in the bottom of an anode diffusion layer of the casing. The manufacturing cost of the cell is reduced, the structure is simplified, the catalytic performance of the catalyst for methanol is high, toxin resistance is high, and the cell performance is improved.

The invention discloses a bacterial blight-resistant japonica rice breeding method. The steps of the breeding method are as follows: (1) selecting a japonica rice variety / line to be improved and an indica rice resistance source resistant to bacterial blight; Improve japonica rice varieties / lines for crude toxin tolerance concentration identification; (3) cross breeding to obtain three-cross F1 hybrids; (4) anther culture for three-cross F1 hybrids; (5) field planting of flowers and seedlings , a number of japonica rice cultivar families with improved resistance to bacterial blight were harvested from a single plant, and different flower culture families were further planted in the field. Using the japonica rice variety / line parent to be improved as a control, the agronomically good shape and bacterial blight resistance were screened. A new strain of japonica rice with improved sex. The breeding method of the present invention can specifically and efficiently introduce the resistance to bacterial blight of indica rice into japonica rice, and the breeding for resistance to bacterial blight can be completed in about 3 years, which significantly shortens the conventional breeding cycle for resistance to bacterial blight, and has greater breeding efficiency. Value.

The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.

This invention relates generally to useful compositions and methods related to plant site-directed recombination. In particular, the invention relates to novel nucleic acid sequences unique to a portion of the sorghum NBS-LRR region, as well as vectors, seeds, plant parts and plants comprising these sequences. Methods to investigate recombination co-factors, and methods to investigate potential herbicides are within the scope. This invention also relates to fungal pathogens of sorghum, particularly Periconia circinata.

The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.