139 results about "Tandem Repeat Sequence" patented technology

The present invention provides devices and methods for detecting or characterizing a nucleic acid analyte without requiring electrophoresis or the direct sequencing of analyte samples or analyte fragments. The device includes a panel or array of double stranded oligonucleotide probes immobilized on a solid support. each probe comprising a nucleotide sequence having a hypervariable number of tandem repeat sequences. Desirably, the specificity of the probes is varied with the location on the panel or array. One strand of each probe is preferably anchored at one terminus to a solid support and the opposite terminus of a second strand is not so anchored. The probes and/or the analyte are labeled by one or more reporter moieties, designed, for example, to allow for visual or instrument based detection of hybridization events.

An anticancer vaccine of recombinant human MOC1-MBP fusion protein is disclosed, in which MBP is used as its adjuvant. The MBP gene and MUC1 gene are fused together. The MBP substituted for other fusion protein to induce CTL reaction. The pMAL-P2 is the carrier for effectively expressing maltose fusion protein. The serial repetitive sequence of MUC1 is inserted to downstream of malE gene.

Novel MUC-1 epitopes outside the VNTR region are identified. In addition, the first agonist epitope of MUC-1 is described. The employment of agonist epitopes in peptide, protein and vector-based vaccine may well aid in the development of effective vaccines for a range of human cancers.

The invention provides a detection device and a detection system. The detection device comprises an acquisition unit, an analysis unit, a check unit and an output unit, wherein the acquisition unit is used for acquiring DNA sample data which is generated by sampling through a DNA sequencer; the analysis unit is used for performing short tandem repeat sequence analysis on the DNA sample data and generating analysis result data; the check unit is used for checking the DNA sample data, verifying whether the DNA sample data has errors, and generating check result data; and the output unit is used for outputting the analysis result data and corresponding check result data. The detection device replaces the manual check process so as to efficiently and accurately finish the identification check work for the identification result and omit the process for analyzing and outputting the short tandem repeat sequence for the DNA sample data with poor check result, and reduces the cost.

Methods are described for construction of long synthetic arrays of DNA repeats, such as alphoid repeats or other repeat sequences. The methods include concatamerization of DNA into short repeats (for instance using rolling circle amplification or directional in vitro ligation), followed by assembling the short repeats into long arrays by homologous recombination during transformation into microbe cells. These methods can be described generally as Recombinational Amplification of Repeats (RAR). The long arrays are engineered centromere-like regions that allow one to construct mammalian artificial chromosomes with a predefined centromeric region structure. Artificial chromosomes, including human artificial chromosomes with a regulated centromere, and methods of their use are also provided

The invention supplies a fluorescence labeling verification system by compounding and expanding 14 short series repeating sequence locus to take personal identification and paternity test. The specific compounding method controls the expanding product in the range of 400 basic groups, and only labeling three fluorescence of the compounding expanding of the 14 locus would be realized. The invention takes a deep research to the gene characteristic of the 14 locus and the allele in five minorities in China, and supplies the compounding expanding verification system. The agent box suited for Chinese throng is researched.

The invention discloses a new kind of ten STR gene loci for Y chromosome and the typing method, which is characterized in that polyacrylamide gel is utilized for electrophoresis typing; the ten STR gene loci for Y chromosome, which can be applied in forensic medicine field, are screened out with silver staining coloration method; furthermore, the allelic ladder of each locus is prepared; a PCR primer and the expansion condition of the Y chromosome STR locus are optimized; wherein the optimization to the PCR primer and the expansion condition and the preparation of allelic ladder can be standardized and simplified, which is suitable for popularization of base unit. The ten STR gene loci for Y chromosome can be applied for individual identification, paternity testing and gene diagnosis in forensic medicine, anthropology, genetics, disease and other field. The new kind of ten STR gene loci for Y chromosome and the typing method has the advantages of wide application prospect, which is particularly suitable for paternity testing in the condition of patrilateral loss such as death or missing in forensic medicine practice and individual identification of mixed stain in rape and gang-rape cases, in particular to the identification to suspect having azoospermia or oligospermia disease.

The invention provides a method for efficiently expressing a multi-copy human epidermal growth factor. The method comprises the steps that restriction enzyme cutting sites are introduced to the upstream and downstream of a gene fragment, a tandem repeat sequence of a mature peptide gene of the human epidermal growth factor is obtained after enzyme cutting and connection are conducted, and the problems that the human epidermal growth factor is low in molecular weight and insufficient in expression quantity are solved; meanwhile, an kex2 enzyme cutting site is introduced to the upstream of the gene fragment of the human epidermal growth factor, part of nucleotide bases are modified at downstream, a carboxyl terminal ELR of an original amino acid sequence is changed into a saccharomycete kex2 enzyme cutting site, it is guaranteed that recombinant expressed target protein is cut into monomers through kex2 enzyme inherent to saccharomycetes, the expression quantity is obviously increased, a natural N-terminal is achieved, the difference that only one amino acid residue exists at the C-terminal is achieved, redundant amino acids do not exist, and the activity is not changed; due to the fact that the human epidermal growth factor is naturally synthesized in pichia pastoris, original native state spatial conformation of the protein can be maintained, and therefore efficient large-scale preparation of the human epidermal growth factor is achieved.

The invention relates to the fields of medical jurisprudence, criminal investigation, evidence identification and the like, and specifically relates to a high-throughput multi-site human short fragment tandem repeat sequence detection kit as well as preparation and application thereof. The detection kit comprises a multiplex-PCR (polymerase chain reaction) primer pool, with human short fragment tandem repeat sequence specificity, labeled by different sample labels, a PCR amplification enzyme free of DNA (deoxyribonucleic acid) extraction, a PCR reaction buffer fluid and optional reference DNA and DNA purified magnetic beads. The detection kit has the characteristics of high resolution, high accuracy and high throughput.

The invention relates to a method for fast detecting common chromosome trisome by quantitative fluorescence PCR, which comprises the following steps: sample collection and processing, DNA extraction, primer design and synthesis, PCR amplified reaction of a first system and a second system, PCR product detection, PCR amplified reaction of a third system and PCR product detection. The invention also provides an accurate and reliable reagent kit for fast detecting the common chromosome trisome by the quantitative fluorescence PCR. The invention is integrated with the sensitivity and the accuracy of the quantitative fluorescence PCR and the extensive and uniform distribution, the polymorphism and the high heterozygosity of a tandem repeat sequence and can be used for detecting the number of common chromosomal disorders of prenatal samples, such as peripheral blood, amniotic fluids, cord blood, tomenta, and the like.

The present invention pertains to the field of immunology and genetic engineering. In particular, the present invention relates to the construction, preparation and use of a recombinant vaccine against foot-and-mouth disease virus. The vaccine comprises a tandem repeat of an antigenic epitope of FMDV VP1 protein, the constant region of the immunoglobulin heavy chain or a functional fragment thereof, and the FMDV 3D protein or an immunogenic fragment thereof. The vaccine can induce protective immune response against FMDV in an animal.