Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-throughput multi-site human short fragment tandem repeat sequence detection kit as well as preparation and application thereof

A technology of tandem repeat sequence and detection kit, which is applied in the fields of criminal investigation, physical evidence identification, and forensic science, can solve the problem of not fully considering the difficulty of removing primer dimers, and achieve the effect of improving the ability of individual identification and reducing the cost of detection.

Active Publication Date: 2017-06-13
IPE BIOTECHNOLOGY CO LTD
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN201210466090.4 Failure to fully consider the problem of difficult removal of primer-dimers caused by the growth of fusion primers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-throughput multi-site human short fragment tandem repeat sequence detection kit as well as preparation and application thereof
  • High-throughput multi-site human short fragment tandem repeat sequence detection kit as well as preparation and application thereof
  • High-throughput multi-site human short fragment tandem repeat sequence detection kit as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0099] Example: Parallel detection of 24 STR loci in 192 samples

[0100] 1. Experimental materials

[0101] Reagents: High-throughput multi-locus human short-fragment tandem repeat (STR) detection kit (high-throughput sequencing method), including: 1) library preparation kit, containing 192 sets of multiplex PCR primer pools labeled with different sample tags , free DNA extraction PCR amplification enzyme, PCR reaction buffer, 9947A control DNA, DNA purification magnetic beads (purchased from Beckman AMpure); 2) sequencing template preparation kit (purchased from Ion Torrent); 3) sequencing kit ( available from Ion Torrent Company).

[0102] Sample: Take whole blood spotted on the filter paper matrix as the sample.

[0103] 2. Experimental steps

[0104] 1. Library preparation

[0105] 1) Sample preparation

[0106] Using a hole puncher with a diameter of 1 mm, punch 190 blood samples into two 96-well PCR plates in a certain order, take 1 blood sample for each sample, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the fields of medical jurisprudence, criminal investigation, evidence identification and the like, and specifically relates to a high-throughput multi-site human short fragment tandem repeat sequence detection kit as well as preparation and application thereof. The detection kit comprises a multiplex-PCR (polymerase chain reaction) primer pool, with human short fragment tandem repeat sequence specificity, labeled by different sample labels, a PCR amplification enzyme free of DNA (deoxyribonucleic acid) extraction, a PCR reaction buffer fluid and optional reference DNA and DNA purified magnetic beads. The detection kit has the characteristics of high resolution, high accuracy and high throughput.

Description

technical field [0001] The present invention relates to the fields of forensic medicine, criminal investigation, physical evidence identification and the like, and specifically relates to a human short-segment tandem repeat sequence detection kit and its preparation and application. The kit has the characteristics of high resolution, high accuracy and high throughput. Background technique [0002] How to track and identify suspects in forensic medicine, criminal investigation, and physical evidence identification; how to identify relevant personnel or victims in crimes, disasters, etc.; And the goal of continuous exploration. Since genetics became a discipline due to the groundbreaking work of G.J. Mendel more than 100 years ago, every progress in genetics has given the above-mentioned work a more solid scientific foundation. [0003] In 1985, the British geneticist Jeffreys (A. Jeffreys) first proposed the so-called "DNA fingerprint", pointing out that certain regions in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2537/143C12Q2563/159C12Q2535/122C12Q2563/143
Inventor 周骋潘雅姣曲保旺
Owner IPE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com