40 results about "Soft tissue engineering" patented technology

The invention belongs to the field of polymer material and biomedical engineering and in particular relates to a method for preparing temperature-sensitive amphipathic graft copolymer with chitosan as the main chain. The method comprises the following steps: with the protection of inert gases such as nitrogen and argon, hydroxide group or aminogroup in chitosan main chain are adopted to trigger ring opening polymerization of cyclic ester monomer; then the obtained terminal hydroxide group or aminogroup of aliphatic polyester are converted to bromine group; the bromine group is taken as macroinitiator to polymerize atomic transfer radical of N-isopropyl acrylamide, hydrophilic allyl monomer, thus obtaining the needed product. The temperature-sensitive amphipathic graft copolymer with chitosan as the main chain is provided with biological degradability, biocompatibility, biological activity and temperature sensibility, can be automatically assembled into stable nano micelle in water, thus enjoying wide application in fields such as drug controlled release carrier, soft tissue engineering support material, immunoassay, memory element switch, biosensor and the like. The synthetic method of the invention is simple and feasible, the raw materials can all be applied to industrialized production, thus enjoying very good value in popularization and application.

Cellfree adipose tissue extracts, implants including the same and methods for the preparation thereof. The extracts and implants are capable of inducing adipogenesis and angiogenesis and are, thus, useful in applications including soft tissue repair and engineering and angiogenesis induction, e.g., in wound healing, treatment of burn injuries and ischemic conditions.

The invention discloses a wound healing dressing containing carrageenan, and a preparation method and application of the wound healing dressing. The wound healing dressing is a porous composite stent with a three-dimensional porous interlayer structure, or a composite thin film, wherein the porous composite stent is obtained by blending collagen or gelatin with carrageenan in a mass ratio of (5: 95) to (95: 5) and then freeze-drying the mixture, and the composite thin film is obtained by directly drying the mixture after blending. The wound healing dressing disclosed by the invention is capable of guaranteeing nutrition supply required by tissues for normal healing, and can be degraded and disappear after the tissues are healed; simultaneously, the degradation product has the effects of promoting cell proliferation and matrix expression, and truly achieving the effect of wound healing. Besides, the porous composite stent and the composite thin film both are capable of providing a stent for other soft tissue engineering while promoting wound healing. The wound healing dressing disclosed by the invention is wide in use; specifically, the wound healing dressing can be used for healing large skin wounds caused by burn or anabrosis, and also can be used as tissue engineering stent materials such as cartilage, cornea and fat; or, the wound healing dressing can be used for other fields such as clinical medicine.

Disclosed is a method of preparing a hollow microcapsule using freezing of macroporous materials including a crosslinked inorganic particle network capable of elastically recovering from a highly compressed deformation state, and use of the same as a scaffold for soft tissue engineering and as a drug delivery system.

The invention, which belongs to the technical field of material performance testing, discloses a test device and method for a sliding friction force of a biological soft tissue material. According to the method, on the basis of the law of conservation of energy, testing of a sliding friction force between soft tissue materials is carried out; and the method is especially suitable for biological soft tissue materials that can not be tested easily currently and include the muscle, skin, cartilage and blood vessel. The testing technique based on a simple principle is easy to operate; the testing precision is high; and the sliding friction characteristic between the biological materials can be reflected effectively. The test device and method have the potential application value in biomaterial testing and soft tissue engineering fields.

The invention discloses a method for preparing three-dimensional chitosan hydrogel by using a high temperature crosslinking method and the obtained chitosan hydrogel. The method comprises the following steps of: (a) dispersing chitosan powder into a deionized water; (b) adjusting the pH of chitosan dispersion obtained in the step (a) to be subacid; (c) carrying out high temperature crosslinking on the solution obtained in the step (b) at the high temperature of 80-125 DEG C; and (d) after the crosslinking reaction is completed, soaking and cleaning an obtained product by using a posttreatment liquid so as to remove a residual acid liquid. The high temperature crosslinking method is simple in the preparation process; the reaction time is short; raw materials are unnecessary for special treatment; highly-toxic chemical substances such as crosslinking agents are not introduced to a preparation course; no any chemical toxicant remains in a final product; and the three-dimensional chitosan hydrogel is even in pore structure, good in connectivity and easy to form, and is very suitably used as a support material of soft tissue engineering such as skin and cartilage.

The invention discloses preparation and application of a linear biodegradable polyester elastomer with a controllable elasticity and shape memory effect. The linear biodegradable polyester elastomer with the controllable elasticity and shape memory effect is formed by conducting copolymerization on a caprolactone monomer containing a lateral cyclic ether structural unit and caprolactone, wherein the molar ratio of the caprolactone monomer containing the lateral cyclic ether structural unit to the caprolactone is 5:95-25:75, and the structural formula can be found in description. The viscoelasticity of the linear biodegradable polyester elastomer with the controllable elasticity and shape memory effect is remarkably improved, the elongation at break can reach 1600% and above, the elastomer can be dissolved in a conventional organic solvent, a three-dimensional porous support can be constructed conveniently through a construction technology of the porous support such as, electrostatic spinning, three-dimensional printing and phase separation, tissue engineering blood vessel scaffold materials, myocardial patches, nervous tissue engineering scaffold materials and the like are prepared, and the preparation and application of the linear biodegradable polyester elastomer can be widely applied to the fields of soft tissue engineering scaffolds, tissue repair and regenerative medicine.

The invention belongs to the field of polymer material and biomedical engineering and particularly relates to a method for preparing dual-sensitivity amphipathic graft copolymer with chitosan as the main chain. The method comprises the following steps: with the protection of inert gases such as nitrogen and argon, hydroxide group or aminogroup in chitosan main chain are adopted to carry out esterification reaction with bromine-containing compound; the hydroxide group or aminogroup are converted into the bromine group; then the bromine group is taken as macroinitiator to polymerize atomic transfer radical of methacrylic acid-N and N-dimethylamino-ethyl methylacrylate monomer to form polymer featuring dual sensitivity of temperature and pH value, thus finally obtaining the dual-sensitivity graft copolymer with chitosan as the main chain. The dual-sensitivity graft copolymer with chitosan as the main chain is provided with biological degradability, biocompatibility, biological activity and pH value sensibility, can be automatically assembled into stable nano micelle in water, thus enjoying wide application in fields such as drug controlled release carrier, soft tissue engineering support material, immunoassay, memory element switch, biosensor and the like. The synthetic method of the invention is simple and feasible, the raw materials can all be applied to industrialized production, thus enjoying very good value in popularization and application.

The present invention provides one kind of skin wound repairing agar/collagen dressing and its preparation process and application. Agar and collagen or gelatin are mixed, and the mixture is freeze dried to obtain porous compound rack or compound film for promoting the repair of skin wound, and the rack may be also used in other soft tissue engineering. The porous compound rack and compound film of present invention has simple operation, low cost and wide use, and may be used in repairing burnt and ulcer skin as well as for cartilage, cornea, fat and soft tissue engineering.

A porous support for cartrilage tissue engineering is prepared from the high-molecular material of polyethanediol, polyamide, or polyacrylic acid type as mould material, polyhydroxy alkanoate as polymer for the porous support chloroform as solvent and water-soluble crystal as pore-forming agent through smelting the said mould material, pouring it into a container in which there is a soft silica gel male mould, obtaining a high-molecular femal mould, filling the mixture of polymer solution and pore-forming agent particles in the said femal mould, closing compacting, baking to remove solvent, dissolving the mould and pore-forming particles, and drying.

The invention discloses a method for preparing elastic wood based on ultraviolet light initiated graft polymerization. The method comprises the following steps: by using balsa wood as a raw material,carrying out mild delignification treatment by using an acidic sodium chlorite solution to obtain a complete wood fiber skeleton; treating the obtained product with a sodium hydroxide alkaline solution; preparing a mixed solution of an acrylamide monomer and a cross-linking agent, and regulating the mixing ratio of the acrylamide monomer and the cross-linking agent; fully mixing the obtained product with an acrylamide monomer through an impregnation method; and putting the obtained product into a mold, and initiating a grafting cross-linking reaction through ultraviolet light with the wavelength of 320-380 nm to obtain polyacrylamide/wood fiber skeleton composite gel, namely the elastic wood. According to the method, the elastic wood can be prepared under the condition that a chemical initiator is not used, and due to the fact that the elastic wood has high biocompatibility, excellent mechanical performance and excellent light transmission performance, the elastic wood has potential application value in the fields of artificial skin, biosensors, soft tissue engineering materials, wearable flexible electronic base materials, stimulus response soft robots and the like.

The invention discloses a preparation method of a chitosan hydrogel stent for tissue engineering. The preparation method comprises the steps of: preparing a crosslinking agent and preparing gel and specifically comprises the following steps of: 1, preparing the crosslinking agent, placing 1,6-diisocyanatohexane and polyethylene glycol in a container according to a mol ratio of (1-2.5):1, reacting for 1-7 days at a temperature of 20-40 DEG C to obtain the crosslinking agent; 2, preparing a chitosan hydrogel stent for tissue engineering, (A) preparing a chitosan water solution with a concentration of 0.1-10 percent in weight ratio; and (B), adding the crosslinking agent in the chitosan water solution to obtain the chitosan hydrogel stent for tissue engineering, wherein the volume proportion of the crosslinking agent to the chitosan water solution is 1000:(1-50). Compared with the prior art, the preparation method has the advantages of simple and controllable process, mild reaction conditions, no need of catalyst, high speed and better property of chitosan hydrogel, and good biocompatibility because infinitesimal chemical crosslinking agent is introduced during crosslinking, and is a better tissue engineering stent material, and is especially suitable for a soft tissue engineering material.

The present invention has developed methods and compositions utilizing the cell surface CD107a (LAMP-1) as a marker that divides bone-forming and fat-forming progenitor/stem cells within human adipose tissue. The present invention is able to partition stromal progenitors for improved bone and soft tissue engineering and therapies.

The particle (1) is suitable for the manufacture of an implantable soft tissue engineering material and comprises: a three-dimensionally warped and branched sheet (2) where (i) the three-dimensionally warped and branched sheet (2) is made from a biocompatible material having a Young's modulus of 1 kPa to 1 GPa; (ii) the three-dimensionally warped and branched sheet (2) has an irregular shape which is encompassed in a virtual three-dimensional envelope (3) having a volume V_E; (iii) the three-dimensionally warped and branched sheet (2) has a mean sheet thickness T; iv) the three-dimensionally warped and branched sheet (2) has a volume V_S; (v) the particle (1) has a Young's modulus of 100 Pa to 15 kPa; and (vi) the particle (1) further comprises a number of protrusions where the three-dimensionally warped and branched sheet (2) reaches the envelope (3); (vii) the particle (1) has a number of interconnected channel-type conduits (5) defined by the branching of the sheet (2) and/or by voids in the sheet (2); and (viii) where the conduits (5) have (a) a mean diameter D_C; and (b) an anisotropicity index of 1.01 to 5.00.