Preparation method of chitosan hydrogel stent for tissue engineering

Abstract

The invention discloses a preparation method of a chitosan hydrogel stent for tissue engineering. The preparation method comprises the steps of: preparing a crosslinking agent and preparing gel and specifically comprises the following steps of: 1, preparing the crosslinking agent, placing 1,6-diisocyanatohexane and polyethylene glycol in a container according to a mol ratio of (1-2.5):1, reacting for 1-7 days at a temperature of 20-40 DEG C to obtain the crosslinking agent; 2, preparing a chitosan hydrogel stent for tissue engineering, (A) preparing a chitosan water solution with a concentration of 0.1-10 percent in weight ratio; and (B), adding the crosslinking agent in the chitosan water solution to obtain the chitosan hydrogel stent for tissue engineering, wherein the volume proportion of the crosslinking agent to the chitosan water solution is 1000:(1-50). Compared with the prior art, the preparation method has the advantages of simple and controllable process, mild reaction conditions, no need of catalyst, high speed and better property of chitosan hydrogel, and good biocompatibility because infinitesimal chemical crosslinking agent is introduced during crosslinking, and is a better tissue engineering stent material, and is especially suitable for a soft tissue engineering material.

Description

technical field [0001] The invention relates to the field of biomedical engineering materials, in particular to a novel method for preparing a chitosan hydrogel scaffold for tissue engineering. Background technique [0002] In recent years, biological tissue engineering has made great achievements in many fields such as cartilage, bone, skin, blood vessels, etc., and related products have entered the stage of clinical research and will soon be used in clinical practice. As the two basic elements of tissue engineering, seed cells and scaffold materials that provide seed cells with adhesion and proliferation carriers are the focus and focus of research. An ideal biological scaffold material should have the following basic characteristics: 1. Good biocompatibility; 2. Easy to process and sterilize; 3. The mechanical properties of the scaffold should meet the requirements of the newly constructed tissue. As far as the source of tissue engineering scaffold materials is concerned...

AI Technical Summary

Problems solved by technology

The chitosan hydrogel formed by chemical gel has stable properties and good mechanical properties, but the disadvantage is that the amount of chemical cross-linking agent used in the cross-linking process is relatively large, which has certain cytotoxicity; Cross-linking agent, its cytotoxicity is small, but its disadvantage is that its properties are not stable enough, and its shape is easy to change with the change of external environment

[0005] Chinese patent CN1460692A discloses an injectable hydrogel scaffold for cartilage tissue engineering. The main component of the gel is polyethylene glycol dimethacrylate. Although Chinese patents CN1278537A and CN1693345A all involve isocyanate crosslinking agent, but it focuses more on the preparation of the crosslinking agent itself. Chinese patent CN1869094A discloses the preparation of chitosan-modified waterborne polyurethane elastomer. The above-mentioned multiple patents either have unreasonable selection of raw materials, unsatisfactory performance, or industrial Complex, poor controllability of the preparation process, therefore, to construct a new chitosan hydrogel preparation method that can combine the advantages of chemical cross-linking and physical cross-linking chitosan hydrogel, so as to better meet the needs of tissue engineering scaffolds. request is very necessary