47 results about "Rabies virus Antibody" patented technology

The invention refers to a kind of detecting reagent box and the manufacturing method, concretely refers to the reagent which is indirect enzyme immune sorption experiment for detecting rabies virus IgG and the manufacturing method. The reagent box compositions are: beforehand enclosed rabies virus antigen enzyme label board, sample diluting solution, HRP-rabies resisting IgG enzyme compound, condensed washer solvent, substrate and stopping liquid. The specificity of the reagent can reach 100%; the sensitivity is 1:640; the accuracy (the variation coefficient) is 6.98%. The reagent uses indirect ELISA to detect the rabies virus IgG antibody.

The invention discloses a kit for detecting a rabies virus antibody, which belongs to the technical field of biological diagnosis. The kit comprises a magnetic bead coupled with a rabies virus antigen, a second antibody combined with the rabies virus antibody and provided with a marker, a rabies virus antibody standard product, a rabies virus antibody quality control product, a sample dilute solution, and a washing solution, wherein the DNA sequence of the rabies virus antigen is as shown by Seq No. 1, and an amino acid sequence of the rabies virus antigen is as shown by Seq No. 2. By adoptingthe kit, a to-be-detected substance can be separated by adopting a tubular magnetic ball, then the detection is performed by adopting a chemical luminescent method, the interference of impurities canbe avoided, the possibility of false positive can be reduced, and the detection sensitivity can be improved.

The invention provides a method for detecting canine rabies virus antibody (IgG) and a detection kit. The kit is composed of a coated plate and a reagent reaction system, and comprises a rabies virus antigen-coated reaction plate, a standard substance, positive contrast serum, a washing lotion (20X), a sample weak solution, an enzyme-marked combination substance, a developer A, a developer B and a stopping solution. The method is characterized in that the method uses an ELISA method to determine that the content of the canine rabies virus antibody (IgG) reaches a absorbance corresponding to a protective level by detecting the standard substance of the kit, and by compared the absorbance of the sample to be detected with the absorbance of the standard substance, the content of the canine rabies virus antibody (IgG) contained in the sample to be detected is judged whether to reach an immunization protective level. The method and the kit can simultaneously detect a lot of samples, and a detection result is remarkably with a result by a neutralization experiment, has high accuracy degree, is suitable for monitoring an immunization inoculation effect and determining an individual immunization state, and can be used for investigating animal eqpidemic diseases.

The invention discloses a test strip for rapidly detecting porcine pseudorabies virus antibody and a preparation method thereof. The inventive test strip consists of a sample pad (4), a glass fiber member (3), a cellulose nitrate membrane (2), a water absorbent pad (1) and a support (5); wherein the cellulose nitrate membrane contains a detection line (6) coated by porcine pseudorabies virus gE protein and a reference line (7) coated with rabbit anti porcine pseudorabies virus antibody; and the glass fiber member is combined with a porcine pseudorabies virus gE protein marked by colloidal gold. The test strip can be prepared in batches and has the advantages of simple process and low production cost. The test strip can rapidly detect porcine pseudorabies virus antibody possibly present in a detection sample, and has the advantages of high sensitivity, good reproducibility, convenient and rapid operation, accurate, visual and easily-determined result. The test strip is suitable for large-batch onsite detection for base layer and sudden events and for epidemic inquisition and performs assistant effect on porcine pseudorabies virus infection diagnosis.

The invention provides a rabies virus antibody (IgG) enzyme-linked immunoassay kit and a detection method thereof. The detection kit is composed of a purified rabies virus antigen coated microporous plate, enzyme labeled SPA and other reagents. The detection method adopts an indirect method principle to detect the rabies virus IgG antibody in human or animal serum or blood plasma, and is suitable for rabies vaccine immunized serology effect evaluation and epidemiology investigation. An enzyme labeled antibody applied in the invention is a Staphylococal protein A (SPA), and the SPA can be combined with an Fc fragment in IgG molecules in human or mammal serum, so the kit provided by the invention has all the characteristics of an ELISA kit, can be used for human rabies virus antibody detection, and can also be used for detecting the immune effect of various species of animals.

This invention relates to one anti-rabies virus antigen gold immune analysis test agent board and its process method, which comprises the following steps: paving glass fiber paper and aqua fortis fiber film on the underlay film of polyethylene board and polychloroethylene underlay film; covering the film onto the test line and comparison line; the test line is added with gold detector ester polymer film; the comparison line side is added with water absorptive pad; the test line is to cover the virus protein. This invention process method is to cover virus protein analysis film by glue gold label rabbit anti-virus IgG multi-clone antigen to process gold detection combination pad.

The invention relates to an anti-rabies virus IgG antibody colloidal gold immunochromatographic assay reagent plate and a preparation method. Glass fiber paper and cellulose nitrate films are laid on a polyethylene plate and a polyvinyl chloride substrate film; the cellulose nitrate film is coated with an assay line and a contrast line; and a gold-labeled probe polyester film is adhered on the assay line side; water-absorbing filter paper is adhered on the contrast line side, wherein the assay line is coated with rabies virus purified antigen, and the contrast line is coated with anti-SPA purified antigen. The reagent plate of the invention has the advantages of rapid detection, high detection accuracy, high specificity, convenient carrying, simple and convenient operation and can be usedfor detecting rabies virus antibodies of various animals and human. The assay reagent plate can be stored at the normal temperature for 1 year without special equipment or apparatus and has high assay repeatability.

The present invention provides pharmaceutical antibody formulations, in particular liquid pharmaceutical formulations comprising anti-rabies virus antibodies. The formulations can be used in the post exposure prophylaxis of rabies.

The invention relates to a production method of recombination human antibody, in particular to a production method of recombination human anti rabies virus antibody, which is characterized in that the recombination antibody is neutralizing antibody specially combined with rabies virus, which is determined by the specific gene sequence in hypervariable region (CDRs) of antibody heavy chain and light chain variable region and can be expressed in procaryotic cell and eukaryotic cell. The CDR area, part or all gene of the antibody can generate the antibody in any expression system of procaryotic cell and eukaryotic cell, to prevent and treat rabies clinically.

The invention discloses a piece of test paper for colloidal gold immunochromatograohic assay of an IgG (immunoglobulin G) antibody of dog anti-rabies virus and a preparation method of the test paper. The test paper comprises a sample absorbing region, a gold-labeled probe region, a solid-phase antibody region, a water absorbing region and a supporting plate, wherein the sample absorbing region, the gold-labeled probe region, the solid-phase antibody region and the water absorbing region are laid on the supporting plate and are partially overlapped in sequence; the sample absorbing region is coated with rabies virus antigen; the gold-labeled probe region is coated with a gold-labeled probe 1 and a gold-labeled probe 2 which are a monoclonal antibody of the colloidal gold-labeled rabies virus and rabbit IgG respectively; the solid-phase antibody region has a control line C1 (coated with a goat anti-rabbit IgG antibody), a testing line T (coated with a goat anti-dog IgG antibody), a control line C2 (coated with a goat anti-rabbit IgG antibody) and a control line C3 (coated with a goat anti-mouse IgG antibody). The test paper disclosed by the invention has the advantages that the test is fast, the accuracy rate is high, the specificity is strong, the test paper is simple and convenient to carry and operate, and the level of the rabies virus antibody is judged according to the color depths of the control lines.

The invention discloses a stable rabies virus humanized antibody combined reagent. The stable rabies virus humanized antibody combined reagent is composed of recombined humanized rabies virus antibodies NC08 and NM57, a buffering agent, a surfactant, an isoosmotic adjusting agent and an antioxidant, and the pH value is 5.5 to 6.5. The stable rabies virus humanized antibody combined reagent is capable of maintaining the stability of the two antibodies for a long term, is high in solubility, is convenient for utilization, and can be used for post-exposure prophylaxis of rabies.

The invention relates to a colloidal gold testing paper card used for detecting rabies virus antibodies of dogs and cats. By simultaneously coating a rabies virus-associated antigen strip and a staphylococcal protein A (SPA) antibody strip on a nitrocellulose membrane, and labeling the SPA by adopting colloidal gold, a universal testing paper card is obtained. The rabies virus antibodies in serums of the dogs and the cats are detected by using an immunochromatography principle so as to fulfill an aim of detecting the rabies virus antibodies of the dogs and the cats. The testing paper card overcomes the shortages of complicated and cumbersome operation, long testing time, requirement on instruments and incapability of detecting the antibodies in different animals by using the same testing paper card in the prior detecting art.

The invention relates to an ELISA (Enzyme-Linked Immunosorbent Assay) kit for detecting human anti-rabies virus antibody and a detecting method thereof. The kit comprises a capture antibody plate and a detection antibody plate. The method adopts the steps of capturing and eluting target antibody to remove the interference of intrinsic protein in a detection sample so as to reduce the probability of nonspecific binding during ELISA detection. The kit can be used for qualitatively or quantitatively detecting the human anti-rabies virus antibody in a human blood specimen, the detecting method is high in recovery rate, precision and linearity when the concentration of a sample is 12.7ng / ml -225ng / ml, and the kit can be used for accurately detecting samples with high specificity and high sensitivity.

The invention discloses a recombinant pseudorabies virus for expressing S1 proteins of porcine epidemic diarrhea viruses. The virus is inserted into S1 genes of optimized porcine epidemic diarrhea viruses, and the nucleotide sequence of the recombinant pseudorabies virus is shown in SEQ ID NO.1 of a sequence table. The invention further discloses a construction method of the recombinant pseudorabies virus and an application thereof in preparing animal vaccines. The recombinant pseudorabies virus is good in safety, and after immunization, the recombinant pseudorabies virus not only can generate antibodies for the mutant pseudorabies virus, but also can generate S1 protein antibodies for the porcine epidemic diarrhea viruses, so that the market prospect is wide.

The invention discloses a card for rapidly and quantitatively detecting a pseudorabies virus antibody, and a use method thereof. The card comprises a detection card shell and a test strip assembled inthe detection card shell; the test strip comprises a plastic bottom plate with pressure-sensitive adhesive; a sample pad, a marker pad, a nitrocellulose film and water-absorbing paper are adhered tothe bottom plate sequentially; the marker pad consists of a carrier base layer and a marker; the marker is one layer of film formed by spray-coating the carrier base layer with a lanthanide fluorescence detection microsphere and a lanthanide fluorescence quality control microsphere; the nitrocellulose film is coated with a pseudorabies virus recombinant antigen serving as a detection line and coated with a rabbit anti-chicken IgY antibody serving as a quality control line; and the marker is a fluorescence detection microsphere marked with pseudorabies virus structure protein gB and gI (or gE)recombinant antigens and a fluorescence quality control microsphere marked with a chicken IgY antibody. The card can detect the pseudorabies antibody on site rapidly and quantitatively, and has higherpractical value and popularization value.

The invention discloses a rabies virus rapid antibody quantitative detection card and an application method thereof. The rabies virus rapid antibody quantitative detection card comprises a detection card housing and a test strip assembled in the detection card housing, wherein the test strip comprises a plastic baseplate with a pressure-sensitive adhesive; a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper are sequentially bonded on the baseplate; the marker pad consists of a carrier base layer and a marker; the marker is a membrane formed by spraying lanthanide fluorescence detection microspheres and lanthanide fluorescence quality control microspheres onto the carrier base layer; the nitrocellulose membrane is coated with a rabies virus recombinant antigen as a detection line and coated with a rabbit anti-chicken IgY antigen as a quality control line; and the marker is fluorescence detection microspheres marked with a rabies virus structural protein G recombinant antigen and fluorescence quality control microspheres marked with a chicken IgY antibody. The rabies virus rapid antibody quantitative detection card can realize field rapid quantitative detectionof a rabies virus antibody and has a high practical value and popularization value.

The present invention provides pharmaceutical antibody formulations, in particular liquid pharmaceutical formulations comprising anti-rabies virus antibodies. The formulations can be used in the post exposure prophylaxis of rabies.

The invention belongs to the technical field of genetic engineering, and discloses a genetically engineered antibody of anti-rabies virus as well as a preparation method and an application of the genetically engineered antibody. The genetically engineered antibody provided by the invention is formed by assembling a heavy-chain variable area of a recombinant anti-rabies virus antibody and a light-chain variable area of the recombinant anti-rabies virus antibody; a leucine zipper is fused in the heavy-chain variable area of the recombinant anti-rabies virus antibody; and a leucine zipper is fused in the light-chain variable area of the recombinant anti-rabies virus antibody. The genetically engineered antibody is high in rabies virus neutralizing capability, is simple in structure and easy to built; high-efficient expression in a prokaryotic system can be realized, and the industrial mass production can be relatively easy to realize.

The invention discloses a method for rapidly and quantitatively detecting a rabies virus antibody by using a rabies virus G protein, a coding gene of the G protein and test paper, and belongs to the technical field of biology. According to the invention, the nucleotide sequence of optimized rabies virus G protein is shown as SEQ NO.1, G protein is obtained through expression and purification, and G protein colloidal gold test paper for detecting rabies virus antibodies is prepared from the G protein. The test paper sequentially comprises a sample pad, a gold pad, a nitrocellulose membrane and a water absorption pad from one end to the other end, which are mutually overlapped and attached to the upper surface of a PVC bottom plate. The invention has the advantages that the rabies virus antibody in dog serum can be detected, the rabies virus antibody in cat serum can also be detected, and the separated serum does not need to be diluted; through quantitative detection, the concentration of the rabies virus antibody can be accurately obtained, and the evaluation on the immune effect of the vaccine is more objective and accurate.

The invention discloses recombinant rabies viruses BNSP-CDV-F and BNSP-CDV-H in which canine distemper virus main immune genes are embedded. The recombinant viruses take a rabies virus strain SAD-B19as a framework, CDV-F and CDV-H genes shown in SEQ ID NO.1 and SEQ ID NO.2 are separately inserted between an N gene and P gene of a recombinant plasmid SAD-19 full-length cDNA, and finally recombinant rabies virus strains BNSP-CDV-F and BNSP-CDV-H are saved through a reverse genetic operation technology. The recombinant viruses BNSP-CDV-F and BNSP-CDV-H can express fusion protein and hemagglutinin protein of canine distemper viruses; after a vaccine prepared by mixing the recombinant viruses is used for immunizing animals, a great number of anti-rabies virus antibodies and main immune gene antibodies resistant to the canine distemper viruses can be induced and generated. The cost of the vaccine can also be reduced, and the recombinant viruses BNSP-CDV-F and BNSP-CDV-H have a good application and popularization prospect.

The invention discloses recombinant equine serum albumin and a preparation method thereof. The nucleotide sequence (shown as SEQ ID NO : 2) of coding equine serum albumin optimized by codon is constructed into an eukaryotic cell inducible expression vector to transfer into eukaryotic cells to perform inducible expression after culturing, and the recombinant equine serum albumin can be obtained through purification. An application of the recombinant equine serum albumin in the preparation of rabies vaccines for immunizing horses is also disclosed. A high anti-rabies antibody is obtained after arabies antigen using the recombinant equine serum albumin as a stabilizing agent immunizes the horses, and antibodies against human serum albumin are not detected. The obtained recombinant equine serum albumin can be used as the stabilizing agent of the rabies vaccines for immunizing the horses by replacing the human serum albumin, so that the recombinant equine serum albumin can be used for producing high-quality equine anti-rabies virus serum.

The invention discloses a rabies virus antibody quantitative detection kit as well as a preparation method and a detection method thereof, belongs to the technical field of antibody detection kits, and solves the problems that the antibody detection accuracy is not high enough and potential safety hazards exist in the prior art. The kit comprises an immunochromatographic detection test strip and asample diluent, the immunochromatographic detection test strip sequentially comprises a sample pad coated with a rabies virus antigen, a marker pad coated with a monoclonal antibody of the rabies virus antigen, a nitrocellulose membrane coated with a detection line and a quality control line and a water absorption pad from one end to the other end, and the sample pad, the marker pad, the nitrocellulose membrane and the water absorption pad are mutually stacked and attached to the upper surface of a back plate. According to the invention, the rabies virus antibody in blood is detected by adopting a competition method, and real antibody numerical values in a blood sample can be directly displayed according to a standard curve in combination with strip reading equipment, so that the detection accuracy and sensitivity are improved.

The invention discloses a rabies virus antibody colloidal gold detection test strip for human and livestock joint detection and a preparation method of the rabies virus antibody colloidal gold detection test strip. The rabies virus antibody colloidal gold detection test strip comprises a colloidal gold labeled binding area and a detection area, wherein a detection line and a quality control line are arranged on the detection area, rabies virus antigens labeled by colloidal gold and albumen protein gold particles labeled by colloidal gold are wrapped by the colloidal gold labeled binding area, staphylococcus albumins are wrapped by the detection line, and ovalbumin antibodies are wrapped by the quality control line. According to the rabies virus antibody colloidal gold detection test strip, the detection cost can be reduced, the detection sensitivity can be improved, false positive can be avoided, and the human and livestock joint detection of rabies virus antibodies is achieved. Furthermore, the instability problem appearing when monoclonal antibodies or polyclonal antibodies are used as the quality control line can be effectively solved through the quality control line, and the rabies virus antibody colloidal gold detection test strip has a great significance in large-batch sample detection, field application and immunized human self-detection and has very large market development potentials.

The invention provides a recombinant rabies virus of a chimeric canine parvovirus VP2 gene, and belongs to the technical field of immunology. The recombinant rabies virus takes a rabies vaccine candidate strain SAD-B19 as a skeleton, and the VP2 gene of a CPV-2a strain currently popular in China is inserted into an SAD-B19 genome; the recombinant rabies virus strain of the chimeric VP2 protein is obtained through rescue, after immunization, high-level anti-rabies virus antibodies and anti-canine parvovirus VP2 antibodies can be induced to be generated, and the vaccine is low in cost, safe and effective.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a preparation method of antigen protein for detecting a rabies virus antibody and a kit. The method comprises the steps: employing a recombinant baculovirus containing a rabies virus G protein expression cassette for infecting insect cells to obtain infected insect cells; culturing and propagating the infected insect cells; extracting antigen protein of the rabies virus antibody from insect cells; and preparing the kit through the antigen protein. The problem that rabies virus whole-virus particles are used as coating antigens in a traditional method due to a fact that the antibody detection result contains a neutralizing antibody and a non-neutralizing antibody is solved. The method can be usedfor large-scale production and detection of other infectious disease positive serum, has no cross reaction, has the advantages of strong specificity, high sensitivity, good repeatability and wide linear range, avoids biological safety risks, and has very strong creativity.

The invention discloses rabies virus antibody test paper and a preparation method thereof and a detection method thereof, which belong to the technical field of antibody test paper. The problems of false positive risk and safety hazard in the prior art are solved. The test paper comprises, from one end to the other end, a sample pad which coats a rabies virus antigen, a gold standard pad which coats a monoclonal antibody against the rabies virus antigen, a nitrocellulose membrane which coats a detection line and a quality control line, and an absorbent pad, wherein the sample pad, the gold standard pad, the nitrocellulose membrane and the absorbent pad overlap each other and are attached to the upper surface of a backboard. The rabies virus antigen coated by the sample pad is rabies virus-like particles. According to the invention, the used labeled antigen is the rabies virus-like particles expressed by insect cells, which avoids false positives; the monoclonal antibody against the rabies virus G protein competes with a neutralizing antibody in the blood to improve the detection accuracy and sensitivity; the virus-like particles are free of nucleic acid components; and biosafety risks produced by the use of whole viruses as marker antigens are eliminated.

The invention discloses a rabies virus antibody detection kit, which relates to the field of antibody detection kits, comprising a kit, the inside of which is provided with a No. Inner tank mechanism; the No. 1 inner tank mechanism includes a No. 1 inner tank, and a side inner wall of the No. 1 inner tank is provided with a No. 1 tooth wall, and a small gear, and one end of the pinion is equipped with a bull gear, and the No. 2 liner mechanism includes a No. 2 liner, and a side outer wall of the No. 2 liner is provided with a No. 2 tooth wall. The present invention effectively solves the problem that the reagent bottle inside the kit will be displaced and causes the risk of the reagent being broken by setting the No. 2 inner tank mechanism; by setting the No. The thickness of the fingers of the medical staff makes it difficult to take the reagent bottle due to technical problems.

The invention discloses a triple gold label detection test strip for swine fever virus, porcine reproductive and respiratory syndrome virus and pseudorabies virus antibody, which comprises a sample pad, a gold standard pad, a nitrocellulose membrane and absorbent paper. Gold-labeled CSFV reactogenic polypeptides, gold-labeled PRRSV reactogenic polypeptides, and gold-labeled pig PRV reactogenic polypeptides are sprayed on the gold-labeled pad; three detection lines and one quality control line are provided on the nitrocellulose membrane, so CSFV reactogenic polypeptides, PRRSV reactogenic polypeptides, and PRV reactogenic polypeptides are immobilized on the three detection lines respectively, and CSFV antibodies, PRRSV antibodies and PRV antibodies are immobilized on the quality inspection lines. The test strip of the present invention has the advantages of multiple, specific, sensitive, easy to use, fast, etc. It can provide efficient and practical antibody detection technology and products for the grassroots of pig breeding in my country, and provide effective technical support for the supervision of epidemic prevention of pig epidemics.