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Kit for detecting rabies virus antibody

The technology of a rabies virus and kit is applied in the field of kits for detecting rabies virus antibodies, which can solve the problems of unsatisfactory dynamic detection of antibodies, inability to quantify, and low detection sensitivity, so as to improve detection sensitivity, avoid interference from impurities, and reduce false positives. positive effect

Active Publication Date: 2018-11-23
GUANGZHOU YOUDI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing test kits for detecting rabies virus antibodies on the market include colloidal gold method and ELISA method. The existing detection kits have some shortcomings: 1) the traditional colloidal gold method cannot be quantified, and the detection sensitivity is low; 2) ELISA method Quantitative detection can be achieved, but the sensitivity still cannot meet the dynamic detection of antibody production after vaccination

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  • Kit for detecting rabies virus antibody
  • Kit for detecting rabies virus antibody
  • Kit for detecting rabies virus antibody

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Embodiment Construction

[0023] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific experiments and accompanying drawings.

[0024] Preparation of rabies virus antigen

[0025] S21) fusion protein construction

[0026] The 1st to 440th amino acid of the glycoprotein outer region of rabies virus is selected as the nitrogen terminal, the 300th to 450th amino acid of the nucleoprotein of rabies virus is selected as the carbon terminal, and the two peptide chains are connected by a flexible linking peptide GGSGG , with 6 His at the C-terminus of the fusion protein; the structural formula of the rabies virus antigen is: RgP(1~440aa)-GGSGG-NP(300~450aa)-6×His, the DNA sequence of the rabies virus antigen is shown in Seq No.1 The amino acid sequence of the rabies virus antigen is shown in Seq No.2; wherein, G represents glycine, S represents serine, and His represents histidin...

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Abstract

The invention discloses a kit for detecting a rabies virus antibody, which belongs to the technical field of biological diagnosis. The kit comprises a magnetic bead coupled with a rabies virus antigen, a second antibody combined with the rabies virus antibody and provided with a marker, a rabies virus antibody standard product, a rabies virus antibody quality control product, a sample dilute solution, and a washing solution, wherein the DNA sequence of the rabies virus antigen is as shown by Seq No. 1, and an amino acid sequence of the rabies virus antigen is as shown by Seq No. 2. By adoptingthe kit, a to-be-detected substance can be separated by adopting a tubular magnetic ball, then the detection is performed by adopting a chemical luminescent method, the interference of impurities canbe avoided, the possibility of false positive can be reduced, and the detection sensitivity can be improved.

Description

technical field [0001] The invention belongs to the technical field of biological diagnosis, in particular to a kit for detecting rabies virus antibody. Background technique [0002] Rabies is a zoonotic disease caused by a virus found in the saliva of infected animals, which is transmitted to pets and humans through bites or open wounds. Rabies virus (RV) belongs to Rhabdoviridae (Rhabdoviridae) lyssavirus genus (Lyssavirus). The causative agent of rabies. [0003] Rabies virus infects the central nervous system, causing brain lesions and eventual death in infected individuals. There may be a gap of two weeks or even months between exposure to the virus and the appearance of clinical symptoms, and rabies virus can be detected in the saliva of animals a few days before the appearance of obvious clinical symptoms. However, infected animals eventually die as the disease progresses; infected bats, raccoons, foxes, skunks, dogs, and cats pose a great danger to humans. Worldw...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/54326G01N33/56983G01N2333/145
Inventor 陈翠翠李来庆梁焕坤宁波郭晓晓刘细潘钟树海刘鹏娥
Owner GUANGZHOU YOUDI BIOTECH CO LTD
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