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Test paper for rapidly detecting pseudorabies antibody and method for making same

A technology of porcine pseudorabies and porcine pseudorabies virus, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long time required for detection, high cost of detection, complicated operation process, etc., and achieve low production cost , Antigen components are stable and uniform, with good reproducibility

Inactive Publication Date: 2008-07-09
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

However, the antigen preparation process of this method must go through cumbersome modes such as virus cell culture and virus purification. The specificity is not high, the stability is poor, and the cost is high; the detection requires special equipment such as a microplate reader. Personnel need to undergo professional training; the operation process is relatively complicated; the time required for detection is relatively long; the cost of detection is high

Method used

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  • Test paper for rapidly detecting pseudorabies antibody and method for making same

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Embodiment 1

[0030] Embodiment 1 The preparation of the test strip for detecting antibodies of the present invention

[0031] 1 Genetic engineering expression porcine pseudorabies gE protein preparation

[0032] (1) Source of material

[0033] The PRV Min A strain was provided by the China Veterinary Drug Control Institute.

[0034] The recipient strain BL21 was preserved by the Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences; the pMD18-T vector, various restriction enzymes and modification enzymes, pGEX-6P-1, Trizol kit, etc. were purchased from Dalian Bao Biological Company; GST label Purification kits were purchased from Mersham Biosciences; IPTG and ampicillin were purchased from Shanghai Sangong.

[0035] (2) Extraction of viral DNA and RT-PCR amplification of genes

[0036] The DNA was extracted according to the instructions of the Trizol kit; a pair of specific primers targeting the PRV gE gene was designed according to the porcine pseudorabie...

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Abstract

The invention discloses a test strip for rapidly detecting porcine pseudorabies virus antibody and a preparation method thereof. The inventive test strip consists of a sample pad (4), a glass fiber member (3), a cellulose nitrate membrane (2), a water absorbent pad (1) and a support (5); wherein the cellulose nitrate membrane contains a detection line (6) coated by porcine pseudorabies virus gE protein and a reference line (7) coated with rabbit anti porcine pseudorabies virus antibody; and the glass fiber member is combined with a porcine pseudorabies virus gE protein marked by colloidal gold. The test strip can be prepared in batches and has the advantages of simple process and low production cost. The test strip can rapidly detect porcine pseudorabies virus antibody possibly present in a detection sample, and has the advantages of high sensitivity, good reproducibility, convenient and rapid operation, accurate, visual and easily-determined result. The test strip is suitable for large-batch onsite detection for base layer and sudden events and for epidemic inquisition and performs assistant effect on porcine pseudorabies virus infection diagnosis.

Description

technical field [0001] The invention relates to a test strip for detecting animal diseases, in particular to a test strip for rapidly detecting antibodies to porcine pseudorabies virus. The invention also relates to a preparation method and an application method of the test strip, belonging to the field of animal disease inspection and quarantine . Background technique [0002] Porcine pseudorabies (Pseudorabies, PR) is a highly contagious and fatal infectious disease caused by porcine herpesvirus type I. Pigs are the main storage host and source of infection of the disease. Healthy pigs can be infected with the disease by direct contact with sick pigs and infected pigs. Adult pigs are often recessively infected; pregnant sows have abortions, stillbirths, weak fetuses, and mummified fetuses; newborn piglets have fever and neurological symptoms, and even die from exhaustion, with a mortality rate of up to 100%. In recent years, the disease has spread continuously, causing h...

Claims

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Application Information

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IPC IPC(8): G01N33/571G01N33/558G01N33/52G01N33/532
Inventor 崔尚金
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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