Rabies virus antibody colloidal gold detection test strip for human and livestock joint detection and preparation method thereof

A technology of rabies virus and colloidal gold, which is applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as unstable quality control line outlets and difficulties in setting quality control lines, so as to achieve large market development potential and reduce testing costs , the effect of reducing requirements and dosage

Inactive Publication Date: 2014-01-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reason why antigen-labeled gold particles are rarely used in most literatures and patents may be that this method has difficulties in setting quality control lines
Many literatures use rabies virus polyclonal antibody as the quality control line. During the experiment, it was found that if the polyantibody corresponding to the antigen is used to coat the quality control line, it will compete with the detection line and may cause the quality control line to be unstable, as shown in the table 2 shown

Method used

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  • Rabies virus antibody colloidal gold detection test strip for human and livestock joint detection and preparation method thereof
  • Rabies virus antibody colloidal gold detection test strip for human and livestock joint detection and preparation method thereof
  • Rabies virus antibody colloidal gold detection test strip for human and livestock joint detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: The structure and principle of the rabies virus antibody detection test strip

[0018] Such as figure 1 As shown, the rabies virus antibody detection test strip provided by the present invention uses a PVC bottom plate as a carrier, and from left to right is a sample loading area, a gold label binding area, a detection area, and a water absorption area. The beginning of the water absorption area covers the end of the detection area; the beginning of the detection area is covered by the end of the gold label binding area; the beginning of the gold label binding area is covered by the end of the sample application area. The detection area is provided with a quality control line and a detection line, and the gold label binding area is coated with a gold label conjugate that can specifically bind to the quality control line and the detection line under specific circumstances.

[0019] The covering material of the absorbent area is absorbent paper, the coverin...

Embodiment 2

[0022] Embodiment 2: Preparation of rabies virus antibody detection test strip

[0023] 1. Firing of colloidal gold

[0024] Colloidal gold solution was prepared by trisodium citrate reduction method. The specific operation steps are: add ultrapure water into the siliconized blue cap bottle, then add chloroauric acid solution, heat to boiling, after boiling, quickly add an appropriate amount of freshly prepared trisodium citrate, and mix well, the color of the solution After turning into wine red, continue to boil for 15-20 minutes, cool to room temperature and store at 2-8°C for later use.

[0025] 2. Preparation of colloidal gold-labeled antigen

[0026] 1) Preparation of colloidal gold-labeled rabies virus antigen: adjust the pH of the gold solution to 7.0-7.5 with potassium carbonate, add the purified whole rabies virus protein, mix gently immediately, let it stand for 5-10 minutes, and then add polyvinyl alcohol To a final concentration of 0.1%-1%, after removing unsta...

Embodiment 3

[0035] Embodiment 3: the usage method of rabies virus antibody detection test strip

[0036] Take the test card out of the aluminum foil bag, place it horizontally on the laboratory bench and mark it. Take 3 μl of serum sample in 1ml of sample diluent, mix thoroughly, then take 70 μl of the diluted sample and add it to the sample hole on the test card, and interpret the test result within 15-30 minutes, and the result will be invalid after 30 minutes.

[0037] Judgment of test results:

[0038] Negative: only the position of the quality control line (C) has a red precipitation band;

[0039] Positive: There are red precipitation bands at the position of quality control line (C) and test line (T), that is, two red bands appear;

[0040] Invalid: There is no red band at the quality control line (C).

[0041] The same amount of rabies virus protein was taken for colloidal gold-labeled and coated detection lines, and the two detection modes were compared to compare the sensitiv...

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Abstract

The invention discloses a rabies virus antibody colloidal gold detection test strip for human and livestock joint detection and a preparation method of the rabies virus antibody colloidal gold detection test strip. The rabies virus antibody colloidal gold detection test strip comprises a colloidal gold labeled binding area and a detection area, wherein a detection line and a quality control line are arranged on the detection area, rabies virus antigens labeled by colloidal gold and albumen protein gold particles labeled by colloidal gold are wrapped by the colloidal gold labeled binding area, staphylococcus albumins are wrapped by the detection line, and ovalbumin antibodies are wrapped by the quality control line. According to the rabies virus antibody colloidal gold detection test strip, the detection cost can be reduced, the detection sensitivity can be improved, false positive can be avoided, and the human and livestock joint detection of rabies virus antibodies is achieved. Furthermore, the instability problem appearing when monoclonal antibodies or polyclonal antibodies are used as the quality control line can be effectively solved through the quality control line, and the rabies virus antibody colloidal gold detection test strip has a great significance in large-batch sample detection, field application and immunized human self-detection and has very large market development potentials.

Description

technical field [0001] The invention relates to a colloidal gold detection test strip, in particular to a colloidal gold immunochromatographic detection test strip for rabies virus antibody used for zoonotic inspection, and also relates to a preparation method of the test strip. Background technique [0002] Rabies is a zoonotic infectious disease caused by rabies virus (RV). Once humans and animals get sick, the fatality rate is nearly 100%. The main route of infection of rabies is that people are bitten by sick or poisonous animals. Among the three infectious diseases with the largest number of deaths in my country, the death toll of rabies is second only to AIDS, and it ranks second after tuberculosis. It can be seen that the prevention and control of rabies is very important. . Rabies virus vaccine can effectively prevent rabies virus infection and is an important means of preventing and treating rabies. And judging whether the protective effect has been produced after ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/558G01N33/56983
Inventor 孟小筌傅振芳赵凌周军
Owner HUAZHONG AGRI UNIV
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