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Dog anti rabies virus antibody colloidal gold immunochromatography assay detection reagent plate and preparation method thereof

A technology for immunochromatographic detection and rabies virus, which is applied in the direction of measuring devices, analysis materials, instruments, etc., can solve the unavoidable problems of radioisotope pollution, etc., and achieve the effects of rapid detection, good detection repeatability, and convenient portability

Active Publication Date: 2007-09-26
WUHAN J H BIO TECH
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Problems solved by technology

However, the existing methods for detecting rabies virus antibodies, including ELISA, fluorescence immunoassay, radioimmunoassay and chemiluminescence, all have corresponding weaknesses.
Although the ELISA method has high sensitivity, it is prone to false positive results
Fluorescence immunoassay, radioimmunoassay and chemiluminescence require certain experimental equipment, and radioimmunoassay cannot avoid the problem of radioisotope contamination

Method used

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  • Dog anti rabies virus antibody colloidal gold immunochromatography assay detection reagent plate and preparation method thereof
  • Dog anti rabies virus antibody colloidal gold immunochromatography assay detection reagent plate and preparation method thereof

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Embodiment Construction

[0017] Canine anti-rabies virus IgG antibody colloidal gold immunochromatography detection reagent plate, the horizontal plane of the reagent plate is in order from bottom to top: sample absorption area 1, gold-labeled antibody area 2, immobilized antibody area 3 and water absorption area 4, sample absorption area 1 Laying is to lay glass fiber membrane on polyethylene board and polyvinyl chloride lining film 5, lay gold standard probe polyester membrane 7 and nitrocellulose membrane 6 successively on glass fiber membrane, and coat on nitrocellulose membrane 6 Detection line 31 and control line 32, gold standard probe polyester film 7 is pasted on the side of detection line 31, absorbent filter paper 8 is pasted on the side of control line 32, wherein detection line 31 is coated with rabies virus glycoprotein, and control line 32 packs The coated goat anti-rabbit IgG purified antibody, the suitable coating amount of rabies virus glycoprotein is 2-3 μg protein, the suitable anti...

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Abstract

This invention relates to one anti-rabies virus antigen gold immune analysis test agent board and its process method, which comprises the following steps: paving glass fiber paper and aqua fortis fiber film on the underlay film of polyethylene board and polychloroethylene underlay film; covering the film onto the test line and comparison line; the test line is added with gold detector ester polymer film; the comparison line side is added with water absorptive pad; the test line is to cover the virus protein. This invention process method is to cover virus protein analysis film by glue gold label rabbit anti-virus IgG multi-clone antigen to process gold detection combination pad.

Description

technical field [0001] The invention relates to a detection reagent plate and a preparation method thereof, in particular to a reagent plate for rapidly detecting dog anti-rabies virus IgG antibody by colloidal gold immunochromatography and a preparation method thereof. Background technique [0002] Dogs are not only used for hunting, detection, guarding, carnivorous and kept as pets, dogs are also one of the important experimental animals widely used in the fields of life science, medicine, pharmacy, chemical industry, agriculture, military, environmental protection, aerospace and bioengineering. With the rapid development of my country's research fields such as life sciences, medicine, and pharmacy, the use of experimental dogs continues to increase, and the quality requirements continue to increase. Production bases for experimental dogs are constantly emerging, but the detection system for experimental dogs has not yet been established. The hazard...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/549
Inventor 钱金宏廖园园
Owner WUHAN J H BIO TECH
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